Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
PRDM14 Antibody (PA5-47820) in ICC/IF
Immunocytochemistry analysis of PRDM14 in immersion fixed BG01V human embryonic stem cells. Samples were incubated in PRDM14 polyclonal antibody (Product # PA5-47820) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Product Details
PA5-47820
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human PRDM14 Ala2-Asp201
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605537
Product Specific Information
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
PRDM14 encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing 14; PR domain-containing protein 14
Gene Aliases: PFM11; PRDM14
UniProt ID: (Human) Q9GZV8
Entrez Gene ID: (Human) 63978
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
cell fate specification
inner cell mass cell fate commitment
transcription, DNA-templated
germ cell development
embryo implantation
fertilization
germ-line stem cell population maintenance
histone H3-R26 methylation
somatic stem cell population maintenance
negative regulation of fibroblast growth factor receptor signaling pathway
regulation of DNA methylation
homeostasis of number of cells within a tissue
inactivation of paternal X chromosome
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support