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PRDM14 Antibody (MA5-24292) in ICC/IF
Immunocytochemistry analysis of PRDM14 in fixed BG01V human embryonic stem cells grown on irradiated MEF cells. Samples were incubated in PRDM14 monoclonal antibody (Product # MA5-24292) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.
Product Details
MA5-24292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
647002
Immunogen
E. coli-derived recombinant human PRDM14 Ala2-Asp201
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2605535
Product Specific Information
In direct ELISAs, no cross-reactivity with recombinant mouse PRDM14, recombinant human (rh) ZNF24, rhZNF143, rhZNF206 or rhZNF281 is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
PRDM14 encodes a member of the PRDI-BF1 and RIZ homology domain containing (PRDM) family of transcriptional regulators. The encoded protein may possess histone methyltransferase activity and plays a critical role in cell pluripotency by suppressing the expression of differentiation marker genes. Expression of this gene may play a role in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing 14; PR domain-containing protein 14
Gene Aliases: PFM11; PRDM14
UniProt ID: (Human) Q9GZV8
Entrez Gene ID: (Human) 63978
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
cell fate specification
inner cell mass cell fate commitment
transcription, DNA-templated
germ cell development
embryo implantation
fertilization
germ-line stem cell population maintenance
histone H3-R26 methylation
somatic stem cell population maintenance
negative regulation of fibroblast growth factor receptor signaling pathway
regulation of DNA methylation
homeostasis of number of cells within a tissue
inactivation of paternal X chromosome
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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