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PRDM16 Antibody (MA5-37723) in WB
Western Blot analysis of PRDM16 using the PRDM16 Monoclonal Antibody (Product # MA5-37723) at a 1:4000 dilution. Lane 1: Jurkat whole cell lysate, Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size : 140 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
MA5-37723
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1740CT793.52.44
Immunogen
recombinant protein between 779-996 amino acids from PRDM16
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897647
Target Information
PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Histone-lysine N-methyltransferase PRDM16; MDS1/EVI1-like gene 1; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; Transcription factor MEL1
Gene Aliases: CMD1LL; KIAA1675; LVNC8; MEL1; PFM13; PRDM16
UniProt ID: (Human) Q9HAZ2
Entrez Gene ID: (Human) 63976
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
neurogenesis
negative regulation of transforming growth factor beta receptor signaling pathway
histone lysine methylation
somatic stem cell population maintenance
regulation of cellular respiration
tongue development
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
white fat cell differentiation
brown fat cell differentiation
palate development
positive regulation of brown fat cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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