Abnova

PRKDC Monoclonal Antibody (2A8)

View all (53) DNA-PK antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

H00005591-M03

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL
-

Immunocytochemistry (ICC/IF)

10 µg/mL
-

ELISA (ELISA)

1 ng/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2A8

Immunogen

PRKDC (NP_008835, 4019 a.a. approximately 4128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: KMLKKGGSWI QEINVAEKNW YPRQKICYAK RKLAGANPAV ITCDELLLGH EKAPAFRDYV AVARGSKDHN IRAQEPESGL SEETQVKCLM DQATDPNILG RTWEGREPWM

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

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Gene Aliases: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7

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UniProt ID: (Human) P78527

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Entrez Gene ID: (Human) 5591

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Function(s)
double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation
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