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PRMT1 Polyclonal Antibody

View all (16) PRMT1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (9)

PRMT1 Antibody in Immunocytochemistry (ICC/IF)

PRMT1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

PRMT1 Antibody (11279-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 11279-1-AP (PRMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PRMT1 Antibody in Immunocytochemistry (ICC/IF)

PRMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRMT1 Antibody in Western Blot (WB)

PRMT1 Antibody in Western Blot (WB)

PRMT1 Antibody in Western Blot (WB)

PRMT1 Antibody in Western Blot (WB)

PRMT1 Antibody in Western Blot (WB)

PRMT1 Antibody in Western Blot (WB)

PRMT1 Antibody in Flow Cytometry (Flow)

Product Image Gallery

PRMT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed A549 cells using 11279-1-AP (PRMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PRMT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma using 11279-1-AP (PRMT1 antibody) at dilution of 1:100 (under 10x lens).

PRMT1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 11279-1-AP) PRMT1 antibody at dilution of 1:6,000 incubated at room temperature for 1.5 hours.

PRMT1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 11279-1-AP) PRMT1 antibody at dilution of 1:4,000 incubated at room temperature for 1.5 hours.

PRMT1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PRMT1 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PRMT1 Antibody in Western Blot (WB) — A549 cells were subjected to SDS PAGE followed by western blot with 11279-1-AP ( PRMT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PRMT1 Antibody in Western Blot (WB) — WB result of PRMT1 antibody (Product # 11279-1-AP) 1:4,000, incubated at room temperature for 1.5 hours with sh-Control and sh-PRMT1 transfected A549 cells.

PRMT1 Antibody in Flow Cytometry (Flow) — 1X10^6 Raji cells were stained with 0.2ug PRMT1 antibody (11279-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Product Details

11279-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PRMT1 Fusion Protein Ag1790 (1-343 aa encoded by BC019268)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEVSCGQAE SSEKPNAEDM TSKDYYFDSY AHFGIHEEML KDEVRTLTYR NSMFHNRHLF KDKVVLDVGS GTGILCMFAA KAGARKVIGI ECSSISDYAV KIVKANKLDH VVTIIKGKVE EVELPVEKVD IIISEWMGYC LFYESMLNTV LYARDKWLAP DGLIFPDRAT LYVTAIEDRQ YKDYKIHWWE NVYGFDMSCI KDVAIKEPLV DVVDPKQLVT NACLIKEVDI YTVKVEDLTF TSPFCLQVKR NDYVHALVAY FNIEFTRCHK RTGFSTSPES PYTHWKQTVF YMEDYLTVKT GEEIFGTIGM RPNAKNNRDL DFTIDLDFKG QLCELSCSTD YRMR (1-343 aa encoded by BC019268)

Target Information

PRMT1 is a member of the protein arginine N-methyltransferase (PRMT) family. Arginine methylation is an irreversible post translational modification which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Increased expression of PRMT1 may play a role in various types of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners.

Cell metabolism

Li Q,Zhang L,Yang Q,Li M,Pan X,Xu J,Zhong C,Yao F,Zhang R,Zhou S,Dai X,Shi X,Dai Y,Xu J,Cheng X,Xiao W,She Z,Wang K,Qian X,Pu L,Zhang P,Wang X

Tue Jun 06 00:00:00 EDT 2023

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners.

Cell metabolism

Li Q,Zhang L,Yang Q,Li M,Pan X,Xu J,Zhong C,Yao F,Zhang R,Zhou S,Dai X,Shi X,Dai Y,Xu J,Cheng X,Xiao W,She Z,Wang K,Qian X,Pu L,Zhang P,Wang X

Tue Jun 06 00:00:00 EDT 2023

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners.

Cell metabolism

Li Q,Zhang L,Yang Q,Li M,Pan X,Xu J,Zhong C,Yao F,Zhang R,Zhou S,Dai X,Shi X,Dai Y,Xu J,Cheng X,Xiao W,She Z,Wang K,Qian X,Pu L,Zhang P,Wang X

Tue Jun 06 00:00:00 EDT 2023

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners.

Cell metabolism

Li Q,Zhang L,Yang Q,Li M,Pan X,Xu J,Zhong C,Yao F,Zhang R,Zhou S,Dai X,Shi X,Dai Y,Xu J,Cheng X,Xiao W,She Z,Wang K,Qian X,Pu L,Zhang P,Wang X

Tue Jun 06 00:00:00 EDT 2023

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners.

Cell metabolism

Li Q,Zhang L,Yang Q,Li M,Pan X,Xu J,Zhong C,Yao F,Zhang R,Zhou S,Dai X,Shi X,Dai Y,Xu J,Cheng X,Xiao W,She Z,Wang K,Qian X,Pu L,Zhang P,Wang X

Tue Jun 06 00:00:00 EDT 2023

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners.

Cell metabolism

Li Q,Zhang L,Yang Q,Li M,Pan X,Xu J,Zhong C,Yao F,Zhang R,Zhou S,Dai X,Shi X,Dai Y,Xu J,Cheng X,Xiao W,She Z,Wang K,Qian X,Pu L,Zhang P,Wang X

Tue Jun 06 00:00:00 EDT 2023

The SMARCA4^R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer.

NPJ precision oncology

Zeng X,Yao B,Liu J,Gong GW,Liu M,Li J,Pan HF,Li Q,Yang D,Lu P,Wu D,Xu P,Chen B,Chen P,Zhang M,Zen K,Jing J,Huang DCS,Chen D,Jiang ZW,Zhao Q

Wed Mar 15 00:00:00 EDT 2023

The SMARCA4^R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer.

NPJ precision oncology

Zeng X,Yao B,Liu J,Gong GW,Liu M,Li J,Pan HF,Li Q,Yang D,Lu P,Wu D,Xu P,Chen B,Chen P,Zhang M,Zen K,Jing J,Huang DCS,Chen D,Jiang ZW,Zhao Q

Wed Mar 15 00:00:00 EDT 2023

The SMARCA4^R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer.

NPJ precision oncology

Zeng X,Yao B,Liu J,Gong GW,Liu M,Li J,Pan HF,Li Q,Yang D,Lu P,Wu D,Xu P,Chen B,Chen P,Zhang M,Zen K,Jing J,Huang DCS,Chen D,Jiang ZW,Zhao Q

Wed Mar 15 00:00:00 EDT 2023

The SMARCA4^R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer.

NPJ precision oncology

Zeng X,Yao B,Liu J,Gong GW,Liu M,Li J,Pan HF,Li Q,Yang D,Lu P,Wu D,Xu P,Chen B,Chen P,Zhang M,Zen K,Jing J,Huang DCS,Chen D,Jiang ZW,Zhao Q

Wed Mar 15 00:00:00 EDT 2023

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein.

Frontiers in molecular neuroscience

Wu C,Shang HF,Wang YJ,Wang JH,Zuo ZX,Lian YN,Liu L,Zhang C,Li XY

Tue May 09 00:00:00 EDT 2023

Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein.

Frontiers in molecular neuroscience

Wu C,Shang HF,Wang YJ,Wang JH,Zuo ZX,Lian YN,Liu L,Zhang C,Li XY

Tue May 09 00:00:00 EDT 2023

Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5.

Toxicology and applied pharmacology

Tian Y,Yang W,Yang R,Zhang Q,Hao L,Bian E,Yang Y,Huang X,Wu Y,Zhang B

Sat Jan 15 00:00:00 EST 2022

PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.

Oncogene

Yin XK,Wang YL,Wang F,Feng WX,Bai SM,Zhao WW,Feng LL,Wei MB,Qin CL,Wang F,Chen ZL,Yi HJ,Huang Y,Xie PY,Kim T,Wang YN,Hou JW,Li CW,Liu Q,Fan XJ,Hung MC,Wan XB

Mon Feb 01 00:00:00 EST 2021

PRMT1-mediated EZH2 methylation promotes breast cancer cell proliferation and tumorigenesis.

Cell death & disease

Li Z,Wang D,Chen X,Wang W,Wang P,Hou P,Li M,Chu S,Qiao S,Zheng J,Bai J

Sat Nov 13 00:00:00 EST 2021

PRMT1-mediated EZH2 methylation promotes breast cancer cell proliferation and tumorigenesis.

Cell death & disease

Li Z,Wang D,Chen X,Wang W,Wang P,Hou P,Li M,Chu S,Qiao S,Zheng J,Bai J

Sat Nov 13 00:00:00 EST 2021

Bioinformatics

Protein Aliases: arginine N-methyltransferase 1; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2; heterogeneous nuclear ribonucleoproteins methyltransferase-like 2; Histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; Interferon receptor 1-bound protein 4; Protein arginine N-methyltransferase 1

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Gene Aliases: 6720434D09Rik; ANM1; AW214366; HCP1; HMT2; HRMT1L2; IR1B4; Mrmt1; PRMT1

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UniProt ID: (Human) Q99873, (Mouse) Q9JIF0, (Rat) Q63009

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Entrez Gene ID: (Human) 3276, (Mouse) 15469, (Rat) 60421

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protein binding methyltransferase activity N-methyltransferase activity protein methyltransferase activity methyl-CpG binding protein-arginine N-methyltransferase activity enzyme binding protein-arginine omega-N asymmetric methyltransferase activity histone methyltransferase activity identical protein binding histone methyltransferase activity (H4-R3 specific) poly(A) RNA binding mitogen-activated protein kinase p38 binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity histone-arginine N-methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity [cytochrome c]-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity snoRNP binding methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity protein-arginine omega-N monomethyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity protein modifying enzyme

Process(es)

regulation of transcription, DNA-templated protein methylation DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest cell surface receptor signaling pathway histone methylation peptidyl-arginine methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine neuron projection development histone H4-R3 methylation positive regulation of erythrocyte differentiation negative regulation of megakaryocyte differentiation positive regulation of hemoglobin biosynthetic process positive regulation of p38MAPK cascade in utero embryonic development methylation peptidyl-arginine N-methylation peptidyl-arginine omega-N-methylation liver regeneration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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