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PRMT4 Antibody (PA5-78328) in IHC (P)
PRMT4 Polyclonal Antibody detects CARM1 protein on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. PRMT4 Polyclonal Antibody (Product # PA5-78328) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-78328
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human CARM1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2736457
Product Specific Information
Predicted Reactivity: Mouse (100%), Rat (100%), Zebrafish (94%), Xenopus laevis (91%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coactivator-associated arginine methyltransferase 1; Histone-arginine methyltransferase CARM1; Protein arginine N-methyltransferase 4
Gene Aliases: CARM1; PRMT4
UniProt ID: (Human) Q86X55
Entrez Gene ID: (Human) 10498
transcription coactivator activity
protein binding
beta-catenin binding
protein methyltransferase activity
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
ligand-dependent nuclear receptor transcription coactivator activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
protein homodimerization activity
transcription regulatory region DNA binding
lysine-acetylated histone binding
protein modifying enzyme
regulation of growth plate cartilage chondrocyte proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
aging
positive regulation of cell proliferation
viral process
histone methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
intracellular estrogen receptor signaling pathway
negative regulation of protein binding
regulation of intracellular estrogen receptor signaling pathway
histone H3-R2 methylation
histone H3-R17 methylation
cellular lipid metabolic process
positive regulation of fat cell differentiation
response to cAMP
endochondral bone morphogenesis
regulation of mRNA binding
negative regulation of dendrite development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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