Invitrogen

PRMT4 Monoclonal Antibody (3H2)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (24) PRMT4 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-15796

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-

ChIP assay (ChIP)

4 µL/10^6
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3H2

Immunogen

Purified recombinant fragment of human CARM1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. not determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11154323

Product Specific Information

MA5-15796 targets CARM1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, Mouse and Rat samples.

The MA5-15796 immunogen is purified recombinant fragment of human CARM1 expressed in E. Coli.

MA5-15796 detects CARM1 which has a predicted molecular weight of approximately 65kDa.

Target Information

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Coactivator-associated arginine methyltransferase 1; coactivator-associated arginine methyltransferase 1 variant 5; Histone-arginine methyltransferase CARM1; protein arginine methyltransferase; Protein arginine N-methyltransferase 4

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Gene Aliases: CARM1; PRMT4

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UniProt ID: (Human) Q86X55, (Mouse) Q9WVG6, (Rat) Q4AE70

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Entrez Gene ID: (Human) 10498, (Mouse) 59035, (Rat) 363026

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Function(s)
transcription coactivator activity protein binding beta-catenin binding protein methyltransferase activity histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity ligand-dependent nuclear receptor transcription coactivator activity protein-arginine omega-N asymmetric methyltransferase activity histone methyltransferase activity (H3-R17 specific) histone methyltransferase activity protein homodimerization activity transcription regulatory region DNA binding lysine-acetylated histone binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme
Process(es)
regulation of growth plate cartilage chondrocyte proliferation transcription, DNA-templated regulation of transcription, DNA-templated DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest aging positive regulation of cell proliferation viral process histone methylation peptidyl-arginine methylation, to asymmetrical-dimethyl arginine intracellular estrogen receptor signaling pathway negative regulation of protein binding regulation of intracellular estrogen receptor signaling pathway histone H3-R2 methylation histone H3-R17 methylation cellular lipid metabolic process positive regulation of fat cell differentiation response to cAMP endochondral bone morphogenesis regulation of mRNA binding negative regulation of dendrite development protein methylation pathogenesis chromatin modification intracellular steroid hormone receptor signaling pathway methylation histone arginine methylation peptidyl-arginine N-methylation mRNA processing RNA splicing
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