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PROK1 Antibody (500-P188GBT-25UG) in ELISA
Sandwich ELISA: To detect hEG-VEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of PROK1 Polyclonal Antibody, Biotin (Product # 500-P188GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech PROK1 Polyclonal Antibody (Product # 500-P188G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEG-VEGF.
Product Details
500-P188GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human EG-VEGF
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929730
Product Specific Information
AA Sequence of recombinant protein: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human EG-VEGF. Anti-Human EG-VEGF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hEG-VEGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EG-VEGF (500-P188G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hEG-VEGF.
Western Blot: To detect Human EG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human EG-VEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P188GBT-1MG will be provided as 2 x 500 µg
Target Information
Endocrine gland-derived vascular endothelial growth factor induces proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. Its expression is induced by hypoxia and is restricted to the steroidogenic glands. Its expression is often complementary to the expression of VEGF, suggesting that these molecules function in a coordinated manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: black mamba toxin-related protein; EG-VEGF; Endocrine-gland-derived vascular endothelial growth factor; Mambakine; Prokineticin-1
Gene Aliases: EGVEGF; PK1; PRK1; PROK1; UNQ600/PRO1186
UniProt ID: (Human) P58294
Entrez Gene ID: (Human) 84432
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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