Proteintech

PROX1 Polyclonal Antibody

PROX1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

PROX1 Antibody (11067-2-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using PROX1 antibody 11067-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Product Image Gallery

Product Details

11067-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 16 publications 16 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

Assay-dependent

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PROX1 Fusion Protein Ag1543 (546-736 aa encoded by BC024201)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This is a rabbit polyclonal antibody raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but modified PROX1 is about 90 kDa.

Immunogen sequence: TAEGLSLSL IKSECGDLQD MSEISPYSGS AMQEGLSPNH LKKAKLMFFY TRYPSSNMLK TYFSDVKFNR CITSQLIKWF SNFREFYYIQ MEKYARQAIN DGVTSTEELS ITRDCELYRA LNMHYNKAND FEVPERFLEV AQITLREFFN AIIAGKDVDP SWKKAIYKVI CKLDSEVPEI FKSPNCLQEL LH (546-736 aa encoded by BC024201)

Target Information

The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extracellular HSP90 promotes differentiation of lens epithelial cells to fiber cells by activating LRP1-YAP-PROX1 axis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li J,Yu J,Huang W,Sang F,Li J,Ren Y,Huang H,Wang M,Li K,Zhang J,Li H,Cui X,Zhang J,Hu M,Yuan F,Guo W,Zhang F,Mu H,Hu Y

Wed Feb 01 00:00:00 EST 2023

Isolation and short-term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study.

Microcirculation (New York, N.Y. : 1994)

Jablon KL,Akerstrom VL,Li M,Braun SE,Norton CE,Castorena-Gonzalez JA

Sat Apr 01 00:00:00 EDT 2023

Isolation and short-term culturing of primary lymphatic endothelial cells from collecting lymphatics: A techniques study.

Microcirculation (New York, N.Y. : 1994)

Jablon KL,Akerstrom VL,Li M,Braun SE,Norton CE,Castorena-Gonzalez JA

Sat Apr 01 00:00:00 EDT 2023

Triptolide inhibits the progression of Glioblastoma U251 cells via targeting PROX1.

Frontiers in oncology

Yuan C,Liao Y,Liao S,Huang M,Li D,Wu W,Quan Y,Li L,Yu X,Si W

Tue Mar 28 00:00:00 EDT 2023

KSHV infection of endothelial precursor cells with lymphatic characteristics as a novel model for translational Kaposi's sarcoma studies.

PLoS pathogens

Tuohinto K,DiMaio TA,Kiss EA,Laakkonen P,Saharinen P,Karnezis T,Lagunoff M,Ojala PM

Sun Jan 01 00:00:00 EST 2023

PROX1 induction by autolysosomal activity stabilizes persister-like state of colon cancer via feedback repression of the NOX1-mTORC1 pathway.

Cell reports

Ohata H,Shiokawa D,Sakai H,Kanda Y,Okimoto Y,Kaneko S,Hamamoto R,Nakagama H,Okamoto K

Tue Jun 27 00:00:00 EDT 2023

Pharmacological inhibition of FOXO1 promotes lymphatic valve growth in a congenital lymphedema mouse model.

Frontiers in cell and developmental biology

Ogunsina O,Banerjee R,Knauer LA,Yang Y

Tue Feb 07 00:00:00 EST 2023

Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis.

Nature communications

Meçe O,Houbaert D,Sassano ML,Durré T,Maes H,Schaaf M,More S,Ganne M,García-Caballero M,Borri M,Verhoeven J,Agrawal M,Jacobs K,Bergers G,Blacher S,Ghesquière B,Dewerchin M,Swinnen JV,Vinckier S,Soengas MS,Carmeliet P,Noël A,Agostinis P

Thu May 19 00:00:00 EDT 2022

Loss of BAF (mSWI/SNF) chromatin-remodeling ATPase Brg1 causes multiple malformations of cortical development in mice.

Human molecular genetics

Jin Y,Gao X,Lu M,Chen G,Yang X,Ren N,Song Y,Hou C,Li J,Liu Q,Gao J

Mon Oct 10 00:00:00 EDT 2022

Effect of dietary L-theanine supplementation on skeletal muscle fiber type transformation in weaning piglets.

Animal biotechnology

Chen X,Chen L,Qin Y,Mao Z,Jia G,Zhao H,Liu G,Huang Z

Thu Dec 01 00:00:00 EST 2022

Mechanisms of Neuropathic Pain and Pain-Relieving Effects of Exercise Therapy in a Rat Neuropathic Pain Model.

Journal of pain research

Sumizono M,Yoshizato Y,Yamamoto R,Imai T,Tani A,Nakanishi K,Nakakogawa T,Matsuoka T,Matsuzaki R,Tanaka T,Sakakima H

Fri Jul 22 00:00:00 EDT 2022

Mechanisms of Neuropathic Pain and Pain-Relieving Effects of Exercise Therapy in a Rat Neuropathic Pain Model.

Journal of pain research

Sumizono M,Yoshizato Y,Yamamoto R,Imai T,Tani A,Nakanishi K,Nakakogawa T,Matsuoka T,Matsuzaki R,Tanaka T,Sakakima H

Fri Jul 22 00:00:00 EDT 2022

In Vitro Induction of Human Dental Pulp Stem Cells to Lymphatic Endothelial Cells.

Cellular reprogramming

Qi S,Ye L,Hu L,Pan J

Mon Aug 01 00:00:00 EDT 2022

Tumor microenvironment remodeling modulates macrophage phenotype in breast cancer lymphangiogenesis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Du Y,Cao M,Liu Y,He Y,Yang C,Zhang G,Fan Y,Gao F

Fri Apr 01 00:00:00 EDT 2022

Ubiquitin C-terminal hydrolase L1 promotes lymph node metastasis in small cell neuroendocrine carcinomas of the cervix.

The Journal of international medical research

Zhang Y,Ding J,Zhang X,Hua K

Fri Apr 01 00:00:00 EDT 2022

Ubiquitin C-terminal hydrolase L1 promotes lymph node metastasis in small cell neuroendocrine carcinomas of the cervix.

The Journal of international medical research

Zhang Y,Ding J,Zhang X,Hua K

Fri Apr 01 00:00:00 EDT 2022

Disrupted in Schizophrenia 1 Regulates Ectopic Neurogenesis in the Mouse Hilus After Pilocarpine-induced Status Epilepticus.

Neuroscience

Chen L,Zhu L,Xu J,Xu P,Han Y,Chang L,Wu Q

Fri Jul 01 00:00:00 EDT 2022

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Nature communications

Wang Y,Luo M,Wang F,Tong Y,Li L,Shu Y,Qiao K,Zhang L,Yan G,Liu J,Ji H,Xie Y,Zhang Y,Gao WQ,Liu Y

Thu Nov 24 00:00:00 EST 2022

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Nature communications

Wang Y,Luo M,Wang F,Tong Y,Li L,Shu Y,Qiao K,Zhang L,Yan G,Liu J,Ji H,Xie Y,Zhang Y,Gao WQ,Liu Y

Thu Nov 24 00:00:00 EST 2022

Neurotensin is an anti-thermogenic peptide produced by lymphatic endothelial cells.

Cell metabolism

Li J,Li E,Czepielewski RS,Chi J,Guo X,Han YH,Wang D,Wang L,Hu B,Dawes B,Jacobs C,Tenen D,Lin SJ,Lee B,Morris D,Tobias A,Randolph GJ,Cohen P,Tsai L,Rosen ED

Tue Jul 06 00:00:00 EDT 2021

Neurotensin is an anti-thermogenic peptide produced by lymphatic endothelial cells.

Cell metabolism

Li J,Li E,Czepielewski RS,Chi J,Guo X,Han YH,Wang D,Wang L,Hu B,Dawes B,Jacobs C,Tenen D,Lin SJ,Lee B,Morris D,Tobias A,Randolph GJ,Cohen P,Tsai L,Rosen ED

Tue Jul 06 00:00:00 EDT 2021

MiR-155-5p suppresses SOX1 to promote proliferation of cholangiocarcinoma via RAF/MEK/ERK pathway.

Cancer cell international

Wang D,Xiong F,Wu G,Liu W,Wang B,Chen Y

Tue Dec 07 00:00:00 EST 2021

ASCL1, NKX2-1, and PROX1 co-regulate subtype-specific genes in small-cell lung cancer.

iScience

Pozo K,Kollipara RK,Kelenis DP,Rodarte KE,Ullrich MS,Zhang X,Minna JD,Johnson JE

Fri Sep 24 00:00:00 EDT 2021

ASCL1, NKX2-1, and PROX1 co-regulate subtype-specific genes in small-cell lung cancer.

iScience

Pozo K,Kollipara RK,Kelenis DP,Rodarte KE,Ullrich MS,Zhang X,Minna JD,Johnson JE

Fri Sep 24 00:00:00 EDT 2021

Heteronemin Suppresses Lymphangiogenesis through ARF-1 and MMP-9/VE-Cadherin/Vimentin.

Biomedicines

Chen HL,Su YC,Chen HC,Su JH,Wu CY,Wang SW,Lin IP,Chen CY,Lee CH

Sun Aug 29 00:00:00 EDT 2021

Generation and characterization of cardiac valve endothelial-like cells from human pluripotent stem cells.

Communications biology

Cheng L,Xie M,Qiao W,Song Y,Zhang Y,Geng Y,Xu W,Wang L,Wang Z,Huang K,Dong N,Sun Y

Mon Sep 06 00:00:00 EDT 2021

Defect of LSS Disrupts Lens Development in Cataractogenesis.

Frontiers in cell and developmental biology

Zhao M,Mei T,Shang B,Zou B,Lian Q,Xu W,Wu K,Lai Y,Liu C,Wei L,Zhu J,Zhang K,Liu Y,Zhao L

Tue Dec 21 00:00:00 EST 2021

Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts.

eLife

Ma R,Martínez-Ramírez AS,Borders TL,Gao F,Sosa-Pineda B

Tue Mar 10 00:00:00 EDT 2020

The cataract-linked RNA-binding protein Celf1 post-transcriptionally controls the spatiotemporal expression of the key homeodomain transcription factors Pax6 and Prox1 in lens development.

Human genetics

Aryal S,Viet J,Weatherbee BAT,Siddam AD,Hernandez FG,Gautier-Courteille C,Paillard L,Lachke SA

Tue Dec 01 00:00:00 EST 2020

Dentate Gyrus Proliferative Responses After Traumatic Brain Injury and Binge Alcohol in Adult Rats.

Neuroscience insights

Ton ST,Adamczyk NS,Gerling JP,Vaagenes IC,Wu JY,Hsu K,O'Brien TE,Tsai SY,Kartje GL

Mon Apr 18 00:00:00 EDT 2022

Oncogenic Herpesvirus Engages Endothelial Transcription Factors SOX18 and PROX1 to Increase Viral Genome Copies and Virus Production.

Cancer research

Gramolelli S,Elbasani E,Tuohinto K,Nurminen V,Günther T,Kallinen RE,Kaijalainen SP,Diaz R,Grundhoff A,Haglund C,Ziegelbauer JM,Pellinen T,Bower M,Francois M,Ojala PM

Sat Aug 01 00:00:00 EDT 2020

Identification of ILK as a critical regulator of VEGFR3 signalling and lymphatic vascular growth.

The EMBO journal

Urner S,Planas-Paz L,Hilger LS,Henning C,Branopolski A,Kelly-Goss M,Stanczuk L,Pitter B,Montanez E,Peirce SM,Mäkinen T,Lammert E

Tue Jan 15 00:00:00 EST 2019

Identification of ILK as a critical regulator of VEGFR3 signalling and lymphatic vascular growth.

The EMBO journal

Urner S,Planas-Paz L,Hilger LS,Henning C,Branopolski A,Kelly-Goss M,Stanczuk L,Pitter B,Montanez E,Peirce SM,Mäkinen T,Lammert E

Tue Jan 15 00:00:00 EST 2019

Native Coronary Collateral Microcirculation Reserve in Rat Hearts.

Journal of the American Heart Association

Liu X,Dong H,Huang B,Miao H,Xu Z,Yuan Y,Qiu F,Chen J,Zhang H,Liu Z,Quan X,Zhu L,Zhang Z

Tue Mar 05 00:00:00 EST 2019

Meningeal lymphatic vessels at the skull base drain cerebrospinal fluid.

Nature

Ahn JH,Cho H,Kim JH,Kim SH,Ham JS,Park I,Suh SH,Hong SP,Song JH,Hong YK,Jeong Y,Park SH,Koh GY

Thu Aug 01 00:00:00 EDT 2019

Paraxial Mesoderm Is the Major Source of Lymphatic Endothelium.

Developmental cell

Stone OA,Stainier DYR

Mon Jul 22 00:00:00 EDT 2019

Suppression of PROX1-mediated TERT expression in hepatitis B viral hepatocellular carcinoma.

International journal of cancer

Kim YJ,Yoo JE,Jeon Y,Chong JU,Choi GH,Song DG,Jung SH,Oh BK,Park YN

Sat Dec 15 00:00:00 EST 2018

Suppression of PROX1-mediated TERT expression in hepatitis B viral hepatocellular carcinoma.

International journal of cancer

Kim YJ,Yoo JE,Jeon Y,Chong JU,Choi GH,Song DG,Jung SH,Oh BK,Park YN

Sat Dec 15 00:00:00 EST 2018

Suppression of PROX1-mediated TERT expression in hepatitis B viral hepatocellular carcinoma.

International journal of cancer

Kim YJ,Yoo JE,Jeon Y,Chong JU,Choi GH,Song DG,Jung SH,Oh BK,Park YN

Sat Dec 15 00:00:00 EST 2018

Suppression of PROX1-mediated TERT expression in hepatitis B viral hepatocellular carcinoma.

International journal of cancer

Kim YJ,Yoo JE,Jeon Y,Chong JU,Choi GH,Song DG,Jung SH,Oh BK,Park YN

Sat Dec 15 00:00:00 EST 2018

Transplantation of artificial human lymphatic vascular tissues fabricated using a cell-accumulation technique and their engraftment in mouse tissue with vascular remodelling.

Journal of tissue engineering and regenerative medicine

Asano Y,Shimoda H,Matsusaki M,Akashi M

Thu Mar 01 00:00:00 EST 2018

Transplantation of artificial human lymphatic vascular tissues fabricated using a cell-accumulation technique and their engraftment in mouse tissue with vascular remodelling.

Journal of tissue engineering and regenerative medicine

Asano Y,Shimoda H,Matsusaki M,Akashi M

Thu Mar 01 00:00:00 EST 2018

Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells.

PloS one

Falero-Perez J,Song YS,Zhao Y,Teixeira L,Sorenson CM,Sheibani N

Wed Apr 03 00:00:00 EDT 2019

Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells.

PloS one

Falero-Perez J,Song YS,Zhao Y,Teixeira L,Sorenson CM,Sheibani N

Wed Apr 03 00:00:00 EDT 2019

The role of fatty acid β-oxidation in lymphangiogenesis.

Nature

Wong BW,Wang X,Zecchin A,Thienpont B,Cornelissen I,Kalucka J,García-Caballero M,Missiaen R,Huang H,Brüning U,Blacher S,Vinckier S,Goveia J,Knobloch M,Zhao H,Dierkes C,Shi C,Hägerling R,Moral-Dardé V,Wyns S,Lippens M,Jessberger S,Fendt SM,Luttun A,Noel A,Kiefer F,Ghesquière B,Moons L,Schoonjans L,Dewerchin M,Eelen G,Lambrechts D,Carmeliet P

Thu Feb 02 00:00:00 EST 2017

The role of fatty acid β-oxidation in lymphangiogenesis.

Nature

Thu Feb 02 00:00:00 EST 2017

Liver-specific Prox1 inactivation causes hepatic injury and glucose intolerance in mice.

FEBS letters

Goto T,Elbahrawy A,Furuyama K,Horiguchi M,Hosokawa S,Aoyama Y,Tsuboi K,Sakikubo M,Hirata K,Masui T,Kubo H,Sakai Y,Uemoto S,Kawaguchi Y

Wed Feb 01 00:00:00 EST 2017

Liver-specific Prox1 inactivation causes hepatic injury and glucose intolerance in mice.

FEBS letters

Goto T,Elbahrawy A,Furuyama K,Horiguchi M,Hosokawa S,Aoyama Y,Tsuboi K,Sakikubo M,Hirata K,Masui T,Kubo H,Sakai Y,Uemoto S,Kawaguchi Y

Wed Feb 01 00:00:00 EST 2017

Liver-specific Prox1 inactivation causes hepatic injury and glucose intolerance in mice.

FEBS letters

Goto T,Elbahrawy A,Furuyama K,Horiguchi M,Hosokawa S,Aoyama Y,Tsuboi K,Sakikubo M,Hirata K,Masui T,Kubo H,Sakai Y,Uemoto S,Kawaguchi Y

Wed Feb 01 00:00:00 EST 2017

Liver-specific Prox1 inactivation causes hepatic injury and glucose intolerance in mice.

FEBS letters

Goto T,Elbahrawy A,Furuyama K,Horiguchi M,Hosokawa S,Aoyama Y,Tsuboi K,Sakikubo M,Hirata K,Masui T,Kubo H,Sakai Y,Uemoto S,Kawaguchi Y

Wed Feb 01 00:00:00 EST 2017

Down-regulation of polycystin in lymphatic malformations: possible role in the proliferation of lymphatic endothelial cells.

Human pathology

Ren JG,Xia HF,Yang JG,Zhu JY,Zhang W,Chen G,Zhao JH,Sun YF,Zhao YF

Sat Jul 01 00:00:00 EDT 2017

Down-regulation of polycystin in lymphatic malformations: possible role in the proliferation of lymphatic endothelial cells.

Human pathology

Ren JG,Xia HF,Yang JG,Zhu JY,Zhang W,Chen G,Zhao JH,Sun YF,Zhao YF

Sat Jul 01 00:00:00 EDT 2017

Diet-induced obesity accelerates blood lactate accumulation of rats in response to incremental exercise to maximum.

American journal of physiology. Regulatory, integrative and comparative physiology

Chen CJ,Liao YH,Lin SY,Yu JX,Li ZJ,Lin YC,Chang GJ,Lin CH,Wong AM

Wed Nov 01 00:00:00 EDT 2017

Diet-induced obesity accelerates blood lactate accumulation of rats in response to incremental exercise to maximum.

American journal of physiology. Regulatory, integrative and comparative physiology

Chen CJ,Liao YH,Lin SY,Yu JX,Li ZJ,Lin YC,Chang GJ,Lin CH,Wong AM

Wed Nov 01 00:00:00 EDT 2017

Hepatology (Baltimore, Md.)

Liu Y,Zhang Y,Wang S,Dong QZ,Shen Z,Wang W,Tao S,Gu C,Liu J,Xie Y,Qin LX

Fri Dec 01 00:00:00 EST 2017

Hepatology (Baltimore, Md.)

Liu Y,Zhang Y,Wang S,Dong QZ,Shen Z,Wang W,Tao S,Gu C,Liu J,Xie Y,Qin LX

Fri Dec 01 00:00:00 EST 2017

Hepatology (Baltimore, Md.)

Liu Y,Zhang Y,Wang S,Dong QZ,Shen Z,Wang W,Tao S,Gu C,Liu J,Xie Y,Qin LX

Fri Dec 01 00:00:00 EST 2017

Cyclin Y inhibits plasticity-induced AMPA receptor exocytosis and LTP.

Scientific reports

Cho E,Kim DH,Hur YN,Whitcomb DJ,Regan P,Hong JH,Kim H,Ho Suh Y,Cho K,Park M

Wed Jul 29 00:00:00 EDT 2015

Mesenchymal status of lymphatic endothelial cell: enlightening treatment of lymphatic malformation.

International journal of clinical and experimental medicine

Cai X,Zhang W,Chen G,Li RF,Sun YF,Zhao YF

Mon Nov 09 00:00:00 EST 2015

Mesenchymal status of lymphatic endothelial cell: enlightening treatment of lymphatic malformation.

International journal of clinical and experimental medicine

Cai X,Zhang W,Chen G,Li RF,Sun YF,Zhao YF

Mon Nov 09 00:00:00 EST 2015

Mesenchymal status of lymphatic endothelial cell: enlightening treatment of lymphatic malformation.

International journal of clinical and experimental medicine

Cai X,Zhang W,Chen G,Li RF,Sun YF,Zhao YF

Mon Nov 09 00:00:00 EST 2015

Mesenchymal status of lymphatic endothelial cell: enlightening treatment of lymphatic malformation.

International journal of clinical and experimental medicine

Cai X,Zhang W,Chen G,Li RF,Sun YF,Zhao YF

Mon Nov 09 00:00:00 EST 2015

The Prox1-Vegfr3 feedback loop maintains the identity and the number of lymphatic endothelial cell progenitors.

Genes & development