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Product Details
81778-1-RR
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2H12
Immunogen
Recombinant protein
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Product Specific Information
Immunogen sequence: TAEGLSLSLI KSECGDLQDM SEISPYSGSA MQEGLSPNHL KKAKLMFFYT RYPSSNMLKT YFSDVKFNRC ITSQLIKWFS NFREFYYIQM EKYARQAIND GVTSTEELSI TRDCELYRAL NMHYNKANDF EVPERFLEVA QITLREFFNA IIAGKDVDPS WKKAIYKVIC KLDSEVPEIF KSPNCLQELL H
Target Information
The expression pattern of the Prox1 homeo box gene suggests that it has a role in a variety of embryonic tissues, including lens. Analysis of mRNA reveals that Prox mRNA is present in many different human tissues and that lens demonstrated the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. The Prox1 gene is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been document to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Homeobox prospero-like protein PROX1; Prospero homeobox protein 1; prospero-related homeobox 1; PROX-1
Gene Aliases: PROX1
UniProt ID: (Human) Q92786
Entrez Gene ID: (Human) 5629
core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription corepressor activity
protein binding
ligand-dependent nuclear receptor binding
transcription regulatory region DNA binding
DBD domain binding
LBD domain binding
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
cell fate determination
kidney development
liver development
positive regulation of endothelial cell proliferation
lymphangiogenesis
lens development in camera-type eye
hepatocyte cell migration
transcription, DNA-templated
brain development
circadian rhythm
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of gene expression
positive regulation of endothelial cell migration
dorsal spinal cord development
dentate gyrus development
cerebellar granule cell differentiation
neural tube development
neuron differentiation
skeletal muscle thin filament assembly
lung development
olfactory placode formation
pancreas development
response to nutrient levels
regulation of circadian rhythm
otic placode formation
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of viral genome replication
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
optic placode formation involved in camera-type eye formation
venous blood vessel morphogenesis
ventricular cardiac myofibril assembly
cardiac muscle cell differentiation
atrial cardiac muscle tissue morphogenesis
ventricular cardiac muscle tissue morphogenesis
retina morphogenesis in camera-type eye
embryonic retina morphogenesis in camera-type eye
endocardium formation
positive regulation of sarcomere organization
ventricular septum morphogenesis
aorta smooth muscle tissue morphogenesis
positive regulation of heart growth
lymphatic endothelial cell differentiation
regulation of transcription involved in lymphatic endothelial cell fate commitment
branching involved in pancreas morphogenesis
lens fiber cell morphogenesis
hepatocyte differentiation
negative regulation of bile acid biosynthetic process
hepatocyte proliferation
acinar cell differentiation
neuronal stem cell population maintenance
positive regulation of cell cycle checkpoint
positive regulation of neural precursor cell proliferation
positive regulation of forebrain neuron differentiation
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