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PRUNE Antibody (PA5-98564) in ICC/IF
Immunofluorescent analysis of PRUNE in HepG2 cells using a PRUNE polyclonal antibody (Product # PA5-98564) at a dilution of 1:135. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-98564
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Exopolyphosphatase PRUNE1 protein (1-168AA)
Conjugate
Form
Liquid
Concentration
3.045 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2813177
Target Information
Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. It plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRES-17; Drosophila-related expressed sequence 17; Exopolyphosphatase PRUNE1; hPrune; HTcD37; Protein prune homolog 1
Gene Aliases: DRES-17; DRES17; H-PRUNE; HTCD37; PRUNE; PRUNE1
UniProt ID: (Human) Q86TP1
Entrez Gene ID: (Human) 58497
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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