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PSA Antibody (A01664-1) in ELISA
Antibody pairs analysis of PSA monoclonal antibodies by Sandwich ELISA: General conditions: 1. Microplate was coated with a capture antibody against PSA, followed by 3 washing cycles. 2. Incubation with free PSA or total PSA followed by 3 washing cycles. 3. Incubation with peroxidase conjugated detection antibody against PSA, followed by 3 washing cycles. 4. Colorimetric determination of bound peroxidase activity.
Product Details
A01664-1
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
8A9B8
Immunogen
human total PSA purified from semenal plasma.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Prostate-specific antigen (PSA) is also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen. It is a serine protease enzyme produced by the cells of the prostate gland. Most of PSA in the blood which is bound to serum proteins is known as total PSA, while a small amount which is not protein bound to is called free PSA. PSA liquifies the semen in the seminal coagulum and allows sperm to swim freely. PSA is often elevated in the presence of prostate cancer and in other prostate disorders. A blood test to measure PSA is considered to be the most effective test currently available for the early detection of prostate cancer. Furthermore, rising levels of PSA over time are associated with both localized and metastatic prostate cancer (CaP).GenScript Human PSA Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with human total PSA purified from semenal plasma.
Target Information
PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and is abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. PSA is used to confirm prostatic acinar cell origin in primary and metastatic carcinoma and to rule out non-prostatic carcinoma mimics.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen; Gamma-seminoprotein; Kallikrein 3; Kallikrein-3; Kallikrein-3 (KLK3); kallikrein-related peptidase 3; KLK-3; P-30 antigen; prostate specific antigen; prostate-specific (APS); Prostate-specific antigen; PSA; Semenogelase; Seminin
Gene Aliases: APS; hK3; KLK2A1; KLK3; PSA
UniProt ID: (Human) P07288
Entrez Gene ID: (Human) 354
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