Invitrogen

PSEN2 Monoclonal Antibody (APS 26)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet
Protocols
Questions & Answers

Product Details

MA1-754

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:20
-

Immunocytochemistry (ICC/IF)

Assay-dependent
-

ELISA (ELISA)

4.5 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
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Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

APS 26

Immunogen

Synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325093

Product Specific Information

MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 1.

MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures. By Western blot, this antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells. In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling.

The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.

Target Information

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AD3LP; AD5; Alzheimer disease 4; E5-1; Presenilin-2; PS-2; PSEN 2; STM-2

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Gene Aliases: AD3L; AD4; Ad4h; ALG-3; Alg3; CMD1V; PS-2; PS2; PSEN2; PSNL2; STM2

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UniProt ID: (Human) P49810, (Rat) O88777, (Mouse) Q61144

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Entrez Gene ID: (Human) 5664, (Rat) 81751, (Mouse) 19165

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Function(s)
endopeptidase activity aspartic-type endopeptidase activity protein binding molecular_function peptidase activity hydrolase activity aspartic endopeptidase activity, intramembrane cleaving aspartic protease
Process(es)
response to hypoxia membrane protein ectodomain proteolysis calcium ion transport Notch signaling pathway Notch receptor processing protein processing membrane protein intracellular domain proteolysis intracellular signal transduction amyloid precursor protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of catalytic activity ephrin receptor signaling pathway beta-amyloid metabolic process negative regulation of transcription from RNA polymerase II promoter cell fate specification somitogenesis positive regulation of receptor recycling negative regulation of protein phosphorylation hair follicle development hematopoietic progenitor cell differentiation T cell activation involved in immune response myeloid leukocyte differentiation proteolysis negative regulation of epidermal growth factor-activated receptor activity regulation of epidermal growth factor-activated receptor activity learning or memory memory protein transport dorsal/ventral neural tube patterning embryonic limb morphogenesis forebrain development negative regulation of protein complex assembly negative regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process endoplasmic reticulum calcium ion homeostasis locomotion regulation of protein binding skin morphogenesis cellular protein metabolic process regulation of synaptic plasticity lung alveolus development thymus development brain morphogenesis positive regulation of coagulation T cell receptor signaling pathway negative regulation of ubiquitin-protein transferase activity protein maturation cardiac muscle contraction negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic signaling pathway embryo development anagen
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