Invitrogen

PSMB5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

6 Published Figures

14 References

View all (11) PSMB5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PSMB5 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

PSMB5 Antibody (PA1-977) in ICC/IF

Immunofluorescence analysis of PSMB5 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSMB5 Polyclonal Antibody (Product # PA1-977) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More

Product Image Gallery

PSMB5 Antibody — Figure 5 ( a ) Immunoblots of proteasome catalytic subunits in H727 cells transfected with siRNA targeting beta5, beta2 or beta5i. ( b ) The catalytic activity of beta5 subunit was decreased in H727 cells transfected with siRNA targeting beta5 compared with H727 cells transfected with scrambled siRNA. ( c ) Effects of siRNA knockdown of beta5, beta5i, or beta2 on Cfz sensitivity (IC 50 values) in H23 and H727 cells. Data are shown as mean +- SD derived from a single non-linear regression based on n = 3 replicates per compound per concentration. ( d ) Heat map showing proteasome catalytic subunit activity profiles of 6 PI-naive patient MM samples purchased from Conversant Biologics and AllCells (left). The numbers represent hydrolysis rates of respective substrates (RFU/min, mean values derived from three technical replicates) and were converted to color format and clustered by using the program ""R"" ( http://www.R-project.org ) (left panel). Carfilzomib (Cfz) cell viability of the same 6 patient MM cells was measured via CellTiter-Glo Luminescent Cell Viability Assay (right panel). {KD}

Product Details

PA1-977

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

View 10 publications 10 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200

View 3 publications 3 publications

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C W(243) I R V S S D N V A D L H D K Y S(259)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2172052

Product Specific Information

PA1-977 detects proteasome 20S X from bovine, mouse and human samples.

PA1-977 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~23 kDa protein representing proteasome 20S X.

The PA1-977 immunizing peptide corresponds to amino acid residues 243-259 from mouse proteasome 20S X. This sequence is 94% conserved in human and rat. PA1-977 immunizing peptide (Cat. # PEP-172) is available for use in neutralization and control procedures.

Target Information

Cyclic nucleotide phosphodiesterases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Non-functional ubiquitin C-terminal hydrolase L1 drives podocyte injury through impairing proteasomes in autoimmune glomerulonephritis.

Nature communications

Reichelt J,Sachs W,Frömbling S,Fehlert J,Studencka-Turski M,Betz A,Loreth D,Blume L,Witt S,Pohl S,Brand J,Czesla M,Knop J,Florea BI,Zielinski S,Sachs M,Hoxha E,Hermans-Borgmeyer I,Zahner G,Wiech T,Krüger E,Meyer-Schwesinger C

PA1-977 was used in Western Blotting to investigate the mechanistic significance of the ubiquitin proteasome system (UPS) in membranous nephropathy (MN) and to uncover that the deubiquitinase Ubiquitin C-terminal hydrolase L1 (UCH-L1) is induced by oxidative stress in podocytes and directly involved in proteasome substrate accumulation, leading to podocyte injury and proteinuria.

Thu Apr 13 00:00:00 EDT 2023

A protein-protein interaction map reveals that the Coxiella burnetii effector CirB inhibits host proteasome activity.

PLoS pathogens

Fu M,Liu Y,Wang G,Wang P,Zhang J,Chen C,Zhao M,Zhang S,Jiao J,Ouyang X,Yu Y,Wen B,He C,Wang J,Zhou D,Xiong X

PA1-977 was used in Immunocytochemistry-immunoflourescence to demonstrate that CirB functions as an inhibitor of host proteasome activity, revealing that proteasome activity is important for intracellular survival of C. burnetii.

Fri Jul 01 00:00:00 EDT 2022

A protein-protein interaction map reveals that the Coxiella burnetii effector CirB inhibits host proteasome activity.

PLoS pathogens

Fu M,Liu Y,Wang G,Wang P,Zhang J,Chen C,Zhao M,Zhang S,Jiao J,Ouyang X,Yu Y,Wen B,He C,Wang J,Zhou D,Xiong X

Fri Jul 01 00:00:00 EDT 2022

Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.

Immune network

Kim S,Park SH,Choi WH,Lee MJ

PA1-977 was used in Western Blotting to show that the most accurate assessment of immunoproteasome activity could be achieved by combining β5i-targeting substrate Ac-ANW-AMC and immunoproteasome inhibitor ONX-0914.

Wed Jun 01 00:00:00 EDT 2022

Oxidative-Stress-Associated Proteostasis Disturbances and Increased DNA Damage in the Hippocampal Granule Cells of the Ts65Dn Model of Down Syndrome.

Antioxidants (Basel, Switzerland)

Puente-Bedia A,Berciano MT,Martínez-Cué C,Lafarga M,Rueda N

PA1-977 was used in Immunohistochemistry (PFA fixed) to indicate that TS GCs show important OS-associated alterations in the systems essential for neuronal homeostasis: DNA damage response and proteostasis, particularly of the proteasome and lysosomal system.

Fri Dec 09 00:00:00 EST 2022

Novel missense mutations in PTCHD1 alter its plasma membrane subcellular localization and cause intellectual disability and autism spectrum disorder.

Human mutation

Halewa J,Marouillat S,Dixneuf M,Thépault RA,Ung DC,Chatron N,Gérard B,Ghoumid J,Lesca G,Till M,Smol T,Couque N,Ruaud L,Chune V,Grotto S,Verloes A,Vuillaume ML,Toutain A,Raynaud M,Laumonnier F

PA1-977 was used in Immunocytochemistry to find that six variants impaired the expression level of the PTCHD1 protein, and were retained in the endoplasmic reticulum suggesting abnormal protein folding.

Thu Jul 01 00:00:00 EDT 2021

Skeletal muscle denervation investigations: selecting an experimental control wisely.

American journal of physiology. Cell physiology

Liu H,Thompson LV

PA1-977 was used in Western Blotting to investigate whether skeletal denervation unilaterally alters the proteolytic system in the contralateral-innervated muscles.

Fri Mar 01 00:00:00 EST 2019

H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth.

Scientific reports

Lee MJ,Miller Z,Park JE,Bhattarai D,Lee W,Kim KB

PA1-977 was used in Western Blotting to assess the use of H727 human bronchial carcinoid cells as a model for de novo carfilzomib resistance.

Mon Mar 11 00:00:00 EDT 2019

Novel Potent Capsid Assembly Modulators Regulate Multiple Steps of the Hepatitis B Virus Life Cycle.

Antimicrobial agents and chemotherapy

Lahlali T,Berke JM,Vergauwen K,Foca A,Vandyck K,Pauwels F,Zoulim F,Durantel D

PA1-977 was used in Immunocytochemistry-immunoflourescence to demonstrate that some capsid assembly modulators could interfere with multiple functions of Hepatitis B virus core protein in the viral life cycle.

Mon Oct 01 00:00:00 EDT 2018

Induction of the Immunoproteasome Subunit Lmp7 Links Proteostasis and Immunity in α-Synuclein Aggregation Disorders.

EBioMedicine

Ugras S,Daniels MJ,Fazelinia H,Gould NS,Yocum AK,Luk KC,Luna E,Ding H,McKennan C,Seeholzer S,Martinez D,Evans P,Brown D,Duda JE,Ischiropoulos H

PA1-977 was used in Western Blotting to examine the role of the Lmp7 subunit of the immunoproteasome in the degradation of α-synuclein aggregates.

Tue May 01 00:00:00 EDT 2018

Denervation-Induced Activation of the Ubiquitin-Proteasome System Reduces Skeletal Muscle Quantity Not Quality.

PloS one

Baumann CW,Liu HM,Thompson LV

PA1-977 was used in western blot investigate the role of the ubiquitin-proteasome system in skeletal muscle

Fri Jul 28 00:00:00 EDT 2017

A selective inhibitor of the immunoproteasome subunit LMP2 induces apoptosis in PC-3 cells and suppresses tumour growth in nude mice.

British journal of cancer

Wehenkel M,Ban JO,Ho YK,Carmony KC,Hong JT,Kim KB

PA1-977 was used in western blot to study the role of immunoproteasome subunit LMP2 in apoptosis of PC-3 cells and tumor growth in nude mice

Tue Jun 26 00:00:00 EDT 2012

Persistent hijacking of brain proteasomes in HIV-associated dementia.

The American journal of pathology

Nguyen TP,Soukup VM,Gelman BB

PA1-977 was used in western blot to study the effect of HIV infection on brain proteasomes and its role in dementia

Mon Feb 01 00:00:00 EST 2010

Reduced ubiquitin C-terminal hydrolase-1 expression levels in dementia with Lewy bodies.

Neurobiology of disease

Barrachina M,Castaño E,Dalfó E,Maes T,Buesa C,Ferrer I

PA1-977 was used in western blot to investigate the changes of UCHL-1 in samples from dementia patients with Lewy bodies

Mon May 01 00:00:00 EDT 2006

Resveratrol promotes clearance of Alzheimer's disease amyloid-beta peptides.

The Journal of biological chemistry

Marambaud P,Zhao H,Davies P

PA1-977 was used in western blot to demonstrate a proteasome-dependent anti-amyloidogenic activity of resveratrol.

Fri Nov 11 00:00:00 EST 2005

Bioinformatics

Protein Aliases: beta-5; Macropain epsilon chain; MGC118075; Multicatalytic endopeptidase complex epsilon chain; proteasome (prosome, macropain) subunit, beta type, 5; proteasome beta 5 subunit; proteasome beta type subunit 5; proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; proteasome subunit beta 5; Proteasome subunit beta type-5; Proteasome subunit beta-5; Proteasome subunit MB1; Proteasome subunit X; proteasome subunit, beta type, 5; PSX large multifunctional protease X; testicular tissue protein Li 153

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Gene Aliases: LMPX; MB1; PSMB5; X

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UniProt ID: (Human) P28074, (Bovine) Q32KL2, (Mouse) O55234

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Entrez Gene ID: (Human) 5693, (Bovine) 534640, (Mouse) 19173

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Function(s)

threonine-type endopeptidase activity protein binding peptidase activity endopeptidase activity hydrolase activity protease

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent proteolysis regulation of cellular amino acid metabolic process response to oxidative stress viral process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway proteasomal ubiquitin-independent protein catabolic process proteolysis involved in cellular protein catabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMB5 Polyclonal Antibody

PSMB5 Antibody (PA1-977) in ICC/IF

Product Details

PA1-977

Western Blot (WB)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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