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PTEN Antibody (PA5-15540) in ICC/IF
Immunocytochemistry analysis of PTEN in MCF-7 cells. Samples were incubated in PTEN polyclonal antibody (Product # PA5-15540) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Product Details
PA5-15540
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 371-403 amino acids from the C-terminal region of human PTEN
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2174181
Target Information
PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; pten tumor suppressor
Gene Aliases: 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN; PTEN1; TEP1
UniProt ID: (Human) P60484
Entrez Gene ID: (Human) 5728
magnesium ion binding
phosphatidylinositol-3-phosphatase activity
phosphoprotein phosphatase activity
protein serine/threonine phosphatase activity
protein tyrosine phosphatase activity
platelet-derived growth factor receptor binding
protein binding
protein tyrosine/serine/threonine phosphatase activity
lipid binding
anaphase-promoting complex binding
phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity
enzyme binding
protein kinase binding
PDZ domain binding
identical protein binding
inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity
phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity
protein phosphatase
regulation of cyclin-dependent protein serine/threonine kinase activity
angiogenesis
negative regulation of protein phosphorylation
regulation of B cell apoptotic process
protein dephosphorylation
phosphatidylinositol biosynthetic process
apoptotic process
neuron-neuron synaptic transmission
synapse assembly
central nervous system development
heart development
aging
response to nutrient
learning or memory
memory
locomotory behavior
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
response to glucose
response to zinc ion
regulation of neuron projection development
negative regulation of phosphatidylinositol 3-kinase signaling
cell migration
dentate gyrus development
central nervous system neuron axonogenesis
negative regulation of cell migration
adult behavior
negative regulation of myelination
regulation of protein stability
negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle
regulation of synaptic transmission, GABAergic
central nervous system myelin maintenance
response to estradiol
regulation of cellular component size
regulation of myeloid cell apoptotic process
response to ATP
multicellular organismal response to stress
social behavior
peptidyl-tyrosine dephosphorylation
response to drug
maternal behavior
negative regulation of apoptotic process
protein kinase B signaling
endothelial cell migration
inositol phosphate metabolic process
response to ethanol
locomotor rhythm
negative regulation of cell size
negative regulation of organ growth
response to arsenic-containing substance
inositol phosphate dephosphorylation
phosphatidylinositol dephosphorylation
platelet-derived growth factor receptor signaling pathway
phosphatidylinositol-mediated signaling
regulation of axon regeneration
cardiac muscle tissue development
forebrain morphogenesis
brain morphogenesis
negative regulation of epithelial cell proliferation
negative regulation of phagocytosis
negative regulation of axonogenesis
protein stabilization
T cell receptor signaling pathway
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of focal adhesion assembly
negative regulation of protein kinase B signaling
rhythmic synaptic transmission
canonical Wnt signaling pathway
synapse maturation
prepulse inhibition
male mating behavior
long-term synaptic potentiation
long term synaptic depression
prostate gland growth
dendritic spine morphogenesis
negative regulation of dendritic spine morphogenesis
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to hypoxia
negative regulation of ribosome biogenesis
negative regulation of cell aging
negative regulation of excitatory postsynaptic potential
presynaptic membrane assembly
postsynaptic density assembly
positive regulation of TRAIL-activated apoptotic signaling pathway
positive regulation of ubiquitin protein ligase activity
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of excitatory postsynaptic potential
negative regulation of synaptic vesicle clustering
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