Invitrogen

PTEN Monoclonal Antibody (2F4C9)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures
5 References
View all (24) PTEN antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

32-5800

Applications
Tested Dilution
Publications

Western Blot (WB)

1-3 µg/mL
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

0.5 µg/mL
View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2F4C9

Immunogen

Recombinant protein derived from the PTEN protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533087

Product Specific Information

32-5800 reacts specifically with the human PTEN protein. Reactivity was confirmed with cell lines which contain endogenous PTEN (NIH3T3, K652, and DU145 prostate cancer line) as well as transfected cell lines (Rat1 and 293T cells). This antibody detects the ~55 kDa PTEN protein and was successfully used in western blotting analysis of PTEN in breast cancer cell lines. No cross-reactivity to any other proteins has been detected.

Target Information

PTEN was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The PTEN gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. PTEN negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. PTEN plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Also, PTEN may be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form of PTEN induces less tumor suppressive ability.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Inositol polyphosphate 3-phosphatase; mitochondrial phosphatase and tensin protein alpha; mitochondrial PTENalpha; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; phosphatase and tensin-like protein; phosphatidylinositol 3; Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; phosphatidylinositol-3; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; pten tumor suppressor

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Gene Aliases: 10q23del; 2310035O07Rik; A130070J02Rik; AI463227; B430203M17Rik; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN; PTEN1; TEP1

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UniProt ID: (Human) P60484, (Mouse) Q542G1

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Entrez Gene ID: (Human) 5728, (Mouse) 19211

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Function(s)
magnesium ion binding phosphatidylinositol-3-phosphatase activity phosphoprotein phosphatase activity protein serine/threonine phosphatase activity protein tyrosine phosphatase activity platelet-derived growth factor receptor binding protein binding protein tyrosine/serine/threonine phosphatase activity lipid binding anaphase-promoting complex binding phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity enzyme binding protein kinase binding PDZ domain binding identical protein binding inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity hydrolase activity phosphatase activity protein phosphatase
Process(es)
regulation of cyclin-dependent protein serine/threonine kinase activity angiogenesis negative regulation of protein phosphorylation regulation of B cell apoptotic process protein dephosphorylation phosphatidylinositol biosynthetic process apoptotic process neuron-neuron synaptic transmission synapse assembly central nervous system development heart development aging response to nutrient learning or memory memory locomotory behavior cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation response to glucose response to zinc ion regulation of neuron projection development negative regulation of phosphatidylinositol 3-kinase signaling cell migration dentate gyrus development central nervous system neuron axonogenesis negative regulation of cell migration adult behavior negative regulation of myelination regulation of protein stability negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle regulation of synaptic transmission, GABAergic central nervous system myelin maintenance response to estradiol regulation of cellular component size regulation of myeloid cell apoptotic process response to ATP multicellular organismal response to stress social behavior peptidyl-tyrosine dephosphorylation response to drug maternal behavior negative regulation of apoptotic process protein kinase B signaling endothelial cell migration inositol phosphate metabolic process response to ethanol locomotor rhythm negative regulation of cell size negative regulation of organ growth response to arsenic-containing substance inositol phosphate dephosphorylation phosphatidylinositol dephosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of axon regeneration cardiac muscle tissue development forebrain morphogenesis brain morphogenesis negative regulation of epithelial cell proliferation negative regulation of phagocytosis negative regulation of axonogenesis protein stabilization T cell receptor signaling pathway positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of focal adhesion assembly negative regulation of protein kinase B signaling rhythmic synaptic transmission canonical Wnt signaling pathway synapse maturation prepulse inhibition male mating behavior long-term synaptic potentiation long term synaptic depression prostate gland growth dendritic spine morphogenesis negative regulation of dendritic spine morphogenesis negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia negative regulation of ribosome biogenesis negative regulation of cell aging negative regulation of excitatory postsynaptic potential presynaptic membrane assembly postsynaptic density assembly positive regulation of TRAIL-activated apoptotic signaling pathway positive regulation of ubiquitin protein ligase activity positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of G1/S transition of mitotic cell cycle positive regulation of excitatory postsynaptic potential negative regulation of synaptic vesicle clustering lipid metabolic process nervous system development dephosphorylation neuron projection development cellular response to decreased oxygen levels positive regulation of apoptotic process regulation of cell cycle positive regulation of apoptotic signaling pathway
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