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PTHLH Antibody (500-P276BT-25UG) in ELISA
Sandwich ELISA: To detect hPTHrP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of PTHLH Polyclonal Antibody, Biotin (Product # 500-P276BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech PTHLH Polyclonal Antibody (Product # 500-P276-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPTHrP.
Product Details
500-P276BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 9.8 kDa Recombinant Human PTHrP
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930204
Product Specific Information
AA Sequence of recombinant protein: AVSEHQLLHD KGKSIQDLRR RFFLHHLIAE IHTAEIRATS EVSPNSKPSP NTKNHPVRFG SDDEGRYLTQ ETNKVETYKE QPLKTP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PTHrP. Anti-Human PTHrP-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hPTHrP by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PTHrP (500-P276) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPTHrP.
Western Blot: To detect hPTHrP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPTHrP is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P276BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, in-frame and downstream of the initiator AUG codon, to give rise to nuclear forms of this hormone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: osteostatin; parathyroid hormone related peptide; parathyroid hormone-like hormone preproprotein; Parathyroid hormone-like protein; parathyroid hormone-like related protein; Parathyroid hormone-related protein; PLP; PTH-related protein; PTH-rP
Gene Aliases: BDE2; HHM; PLP; PTHLH; PTHR; PTHRP
UniProt ID: (Human) P12272
Entrez Gene ID: (Human) 5744
skeletal system development
osteoblast development
adenylate cyclase-activating G-protein coupled receptor signaling pathway
cell-cell signaling
female pregnancy
positive regulation of cell proliferation
negative regulation of cell proliferation
epidermis development
regulation of gene expression
positive regulation of cAMP biosynthetic process
negative regulation of chondrocyte differentiation
cAMP metabolic process
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