Invitrogen

PU.1 Monoclonal Antibody (E.388.3)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

7 References

View all (33) PU.1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PU.1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

PU.1 Antibody (MA5-15064) in ICC/IF

Immunofluorescence analysis of PU.1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PU.1 Rabbit Monoclonal Antibody (E.388.3) (Product # MA5-15064) at 1:150 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 48... View More

PU.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PU.1 Antibody in Western Blot, ChIP Assay (WB, ChIP)

PU.1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

PU.1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of PU.1 was observed in K-562, Daudi, RAW 264.7 in comparison to HeLa and 3T3-L1 using Anti-PU.1 Monoclonal Antibody (E.388.3) (Product # MA5-15064) in Western Blot. {RE}

Product Details

MA5-15064

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:150-1:200

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.388.3

Immunogen

Synthetic peptide corresponding to the sequence of human PU.1 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

8 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986949

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

KDM6A Lysine Demethylase Directs Epigenetic Polarity of MDSCs during Murine Sepsis.

Journal of innate immunity

Bah I,Alkhateeb T,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Published figure using PU.1 monoclonal antibody (Product # MA5-15064) in ChIP assay

Wed Apr 06 00:00:00 EDT 2022

KDM6A Lysine Demethylase Directs Epigenetic Polarity of MDSCs during Murine Sepsis.

Journal of innate immunity

Bah I,Alkhateeb T,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Published figure using PU.1 monoclonal antibody (Product # MA5-15064) in ChIP assay

Wed Apr 06 00:00:00 EDT 2022

Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis.

Frontiers in immunology

Bah I,Youssef D,Yao ZQ,McCall CE,El Gazzar M

MA5-15064 was used in ChIP Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to suggest that epigenetic targeting of MDSCs in human sepsis might resolve post-sepsis immunosuppression and improve sepsis survival.

Mon Mar 28 00:00:00 EDT 2022

Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis.

Frontiers in immunology

Bah I,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Mon Mar 28 00:00:00 EDT 2022

Inhibiting KDM6A Demethylase Represses Long Non-Coding RNA Hotairm1 Transcription in MDSC During Sepsis.

Frontiers in immunology

Bah I,Youssef D,Yao ZQ,McCall CE,El Gazzar M

Mon Mar 28 00:00:00 EDT 2022

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

MA5-15064 was used in ChIP assay to suggest that the inflammatory differences seen in SP-R210L-deficient macrophages are a result of transcriptional differences that are mediated by epigenetic changes brought about by differential expression of the SP-R210 isoforms.

Mon Nov 01 00:00:00 EDT 2021

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

Mon Nov 01 00:00:00 EDT 2021

TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells.

Nature immunology

Gerlach K,Hwang Y,Nikolaev A,Atreya R,Dornhoff H,Steiner S,Lehr HA,Wirtz S,Vieth M,Waisman A,Rosenbauer F,McKenzie AN,Weigmann B,Neurath MF

MA5-15064 was used in immunohistochemistry - frozen section to study the role of TH9 cells in ulcerative colitis

Tue Jul 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: 31 kDa transforming protein; 31 kDa-transforming protein; 31kDa Transforming Protein; hCG_25181; hematopoietic transcription factor PU.1; SFFV proviral integration 1 protein; SPIA; spleen focus forming virus (SFFV) proviral integration oncogene spi1; Transcription factor PU.1; Transcription Factor spi1

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Gene Aliases: Dis-1; Dis1; OF; PU.1; Sfpi-1; SFPI1; SPI-1; SPI-A; SPI1; Tcfpu1; Tfpu.1

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UniProt ID: (Human) P17947, (Mouse) P17433

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Entrez Gene ID: (Human) 6688, (Mouse) 20375

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA binding protein binding NFAT protein binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding sequence-specific DNA binding winged helix/forkhead transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter lymphoid progenitor cell differentiation transcription from RNA polymerase II promoter lymphocyte differentiation erythrocyte differentiation macrophage differentiation granulocyte differentiation somatic stem cell population maintenance myeloid dendritic cell differentiation histone H3 acetylation hypermethylation of CpG island negative regulation of MHC class II biosynthetic process regulation of erythrocyte differentiation negative regulation of gene expression, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter anatomical structure regression cellular response to ethanol negative regulation of histone H4 acetylation apoptotic process involved in patterning of blood vessels positive regulation of pri-miRNA transcription from RNA polymerase II promoter vasculature development myeloid leukocyte differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PU.1 Monoclonal Antibody (E.388.3)

PU.1 Antibody (MA5-15064) in ICC/IF

Product Details

MA5-15064

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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