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Product Details
PA1-4277
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: E(101) Q H L E S P V P S A P G A L A G(117)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2064549
Product Specific Information
PA1-4277 detects alpha- and beta-PUMA from human samples.
PA1-4277 has been successfully used in Western blot procedures. By Western blot this antibody recognizes both forms of the PUMA protein found at ~20 kDa and ~36 kDa in transfected U20S cells.
PA1-4277 immunizing peptide corresponds to amino acid residues E(101) Q H L E S P V P S A P G A L A G(117) of the human PUMA-a form of the PUMA protein.
Target Information
PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Bcl-2-binding component 3, isoforms 1/2; Bcl-2-binding component 3, isoforms 3/4; FLJ42994; JFY-1; p53 up-regulated modulator of apoptosis
Gene Aliases: BBC3; JFY-1; JFY1; PUMA
UniProt ID: (Human) Q96PG8
Entrez Gene ID: (Human) 27113
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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