Proteintech

PYGL Polyclonal Antibody

PYGL Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 30

PYGL Antibody (15851-1-AP) in ICC/IF

Immunofluorescent analysis of HeLa cells using 15851-1-AP (PYGL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PYGL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PYGL Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15851-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rodent

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PYGL Fusion Protein Ag8664 (499-846 aa encoded by BC009895)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GLAELIAEK IGEDYVKDLS QLTKLHSFLG DDVFLRELAK VKQENKLKFS QFLETEYKVK INPSSMFDVQ VKRIHEYKRQ LLNCLHVITM YNRIKKDPKK LFVPRTVIIG GKAAPGYHMA KMIIKLITSV ADVVNNDPMV GSKLKVIFLE NYRVSLAEKV IPATDLSEQI STAGTEASGT GNMKFLNGAL TIGTMDGANV EMAEEAGEEN LFIFGMRIDD VAALDKKGYE AKEYYEALPE LKLVIDQIDN GFFSPKQPDL FKDIINMLFY HDRFKVFADY EAYVKCQDKV SQLYMNPKAW NTMVLKNIAA SGKFSSDRTI KEYAQNIWNV EPSDLKISLS NESNKVNGN (499-846 aa encoded by BC009895)

Target Information

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of hepatic phosphoenolpyruvate carboxykinase 1 dysregulates metabolic responses to acute exercise but enhances adaptations to exercise training in mice.

American journal of physiology. Endocrinology and metabolism

Rome FI,Shobert GL,Voigt WC,Stagg DB,Puchalska P,Burgess SC,Crawford PA,Hughey CC

Sun Jan 01 00:00:00 EST 2023

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer.

International journal of biological sciences

Ji Q,Li H,Cai Z,Yuan X,Pu X,Huang Y,Fu S,Chu L,Jiang C,Xue J,Zhang X,Li R

Wed Apr 19 00:00:00 EDT 2023

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer.

International journal of biological sciences

Ji Q,Li H,Cai Z,Yuan X,Pu X,Huang Y,Fu S,Chu L,Jiang C,Xue J,Zhang X,Li R

Wed Apr 19 00:00:00 EDT 2023

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer.

International journal of biological sciences

Ji Q,Li H,Cai Z,Yuan X,Pu X,Huang Y,Fu S,Chu L,Jiang C,Xue J,Zhang X,Li R

Wed Apr 19 00:00:00 EDT 2023

Glycogen Storage Disease Phenotypes Accompanying the Perturbation of the Methionine Cycle in NDRG3-Deficient Mouse Livers.

Cells

Sohn HA,Lee DC,Park A,Kang M,Yoon BH,Lee CH,Kim YH,Oh KJ,Kim CY,Park SH,Koo H,Kim HC,Yoon WK,Lim DS,Kim D,Park KC,Yeom YI

Wed May 04 00:00:00 EDT 2022

Targeting SLC7A11 improves efferocytosis by dendritic cells and wound healing in diabetes.

Nature

Maschalidi S,Mehrotra P,Keçeli BN,De Cleene HKL,Lecomte K,Van der Cruyssen R,Janssen P,Pinney J,van Loo G,Elewaut D,Massie A,Hoste E,Ravichandran KS

Wed Jun 01 00:00:00 EDT 2022

Candida tropicalis induces NLRP3 inflammasome activation via glycogen metabolism-dependent glycolysis and JAK-STAT1 signaling pathway in myeloid-derived suppressor cells to promote colorectal carcinogenesis.

International immunopharmacology

Zhang Z,Chen Y,Yin Y,Chen Y,Chen Q,Bing Z,Zheng Y,Hou Y,Shen S,Chen Y,Wang T

Thu Dec 01 00:00:00 EST 2022

MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

iScience

Ding DX,Wang Y,Yan W,Fu WN

Fri Mar 18 00:00:00 EDT 2022

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.

Journal of immunology (Baltimore, Md. : 1950)

Fan TW,Daneshmandi S,Cassel TA,Uddin MB,Sledziona J,Thompson PT,Lin P,Higashi RM,Lane AN

Tue Nov 01 00:00:00 EDT 2022

Metabolic alterations and mitochondrial dysfunction underlie hepatocellular carcinoma cell death induced by a glycogen metabolic inhibitor.

Biochemical pharmacology

Barot S,Stephenson OJ,Priya Vemana H,Yadav A,Bhutkar S,Trombetta LD,Dukhande VV

Thu Sep 01 00:00:00 EDT 2022

Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat.

Metabolism: clinical and experimental

Liu Z,Zhang L,Qian C,Zhou Y,Yu Q,Yuan J,Lv Y,Zhang L,Chang X,Li Y,Liu Y

Tue Nov 01 00:00:00 EDT 2022

Liver fibrosis alters the molecular structures of hepatic glycogen.

Carbohydrate polymers

Wan Y,Hu Z,Liu Q,Wang L,Sullivan MA,Gilbert RG

Tue Feb 15 00:00:00 EST 2022

Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction.

Molecular metabolism

Rome FI,Hughey CC

Fri Apr 01 00:00:00 EDT 2022

Human hair follicles operate an internal Cori cycle and modulate their growth via glycogen phosphorylase.

Scientific reports

Figlak K,Williams G,Bertolini M,Paus R,Philpott MP

Thu Oct 21 00:00:00 EDT 2021

Human hair follicles operate an internal Cori cycle and modulate their growth via glycogen phosphorylase.

Scientific reports

Figlak K,Williams G,Bertolini M,Paus R,Philpott MP

Thu Oct 21 00:00:00 EDT 2021

Brain-Type Glycogen Phosphorylase Is Crucial for Astrocytic Glycogen Accumulation in Chronic Social Defeat Stress-Induced Depression in Mice.

Frontiers in molecular neuroscience

Zhu Y,Fan Z,Zhao Q,Li J,Cai G,Wang R,Liang Y,Lu N,Kang J,Luo D,Tao H,Li Y,Huang J,Wu S

Fri Feb 11 00:00:00 EST 2022

Fgf15 Neurons of the Dorsomedial Hypothalamus Control Glucagon Secretion and Hepatic Gluconeogenesis.

Diabetes

Picard A,Metref S,Tarussio D,Dolci W,Berney X,Croizier S,Labouebe G,Thorens B

Thu Jul 01 00:00:00 EDT 2021

Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders.

EMBO molecular medicine

Soria LR,Gurung S,De Sabbata G,Perocheau DP,De Angelis A,Bruno G,Polishchuk E,Paris D,Cuomo P,Motta A,Orford M,Khalil Y,Eaton S,Mills PB,Waddington SN,Settembre C,Muro AF,Baruteau J,Brunetti-Pierri N

Fri Feb 05 00:00:00 EST 2021

Chronic liver disease and impaired hepatic glycogen metabolism in argininosuccinate lyase deficiency.

JCI insight

Burrage LC,Madan S,Li X,Ali S,Mohammad M,Stroup BM,Jiang MM,Cela R,Bertin T,Jin Z,Dai J,Guffey D,Finegold M,Nagamani S,Minard CG,Marini J,Masand P,Schady D,Shneider BL,Leung DH,Bali D,Lee B

Thu Feb 27 00:00:00 EST 2020

The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma.

Clinical proteomics

Lu Y,Luo G,Zhu S,Wang X,Chen Y,Dong Z,Wang S,Ma J,Deng H,Wu D,Dong J

Wed Apr 13 00:00:00 EDT 2022

The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma.

Clinical proteomics

Lu Y,Luo G,Zhu S,Wang X,Chen Y,Dong Z,Wang S,Ma J,Deng H,Wu D,Dong J

Wed Apr 13 00:00:00 EDT 2022

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

The Journal of investigative dermatology

Gu Z,Zhang H,Guo X,Cao Y

Sat Aug 01 00:00:00 EDT 2020

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

The Journal of investigative dermatology

Gu Z,Zhang H,Guo X,Cao Y

Sat Aug 01 00:00:00 EDT 2020

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Cell metabolism

Curtis M,Kenny HA,Ashcroft B,Mukherjee A,Johnson A,Zhang Y,Helou Y,Batlle R,Liu X,Gutierrez N,Gao X,Yamada SD,Lastra R,Montag A,Ahsan N,Locasale JW,Salomon AR,Nebreda AR,Lengyel E

Tue Jan 08 00:00:00 EST 2019

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Cell metabolism

Curtis M,Kenny HA,Ashcroft B,Mukherjee A,Johnson A,Zhang Y,Helou Y,Batlle R,Liu X,Gutierrez N,Gao X,Yamada SD,Lastra R,Montag A,Ahsan N,Locasale JW,Salomon AR,Nebreda AR,Lengyel E

Tue Jan 08 00:00:00 EST 2019

Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.

Journal of cellular physiology

Wang Z,Jiang SF,Cao J,Liu K,Xu SH,Arfat Y,Guo QL,Chang H,Goswami N,Hinghofer-Szalkay H,Gao YF

Thu Aug 01 00:00:00 EDT 2019

Novel findings on ultrastructural protection of skeletal muscle fibers during hibernation of Daurian ground squirrels: Mitochondria, nuclei, cytoskeleton, glycogen.

Journal of cellular physiology

Wang Z,Jiang SF,Cao J,Liu K,Xu SH,Arfat Y,Guo QL,Chang H,Goswami N,Hinghofer-Szalkay H,Gao YF

Thu Aug 01 00:00:00 EDT 2019

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

The Journal of clinical investigation

Lanaspa MA,Andres-Hernando A,Orlicky DJ,Cicerchi C,Jang C,Li N,Milagres T,Kuwabara M,Wempe MF,Rabinowitz JD,Johnson RJ,Tolan DR

Fri Jun 01 00:00:00 EDT 2018

Ketohexokinase C blockade ameliorates fructose-induced metabolic dysfunction in fructose-sensitive mice.

The Journal of clinical investigation

Lanaspa MA,Andres-Hernando A,Orlicky DJ,Cicerchi C,Jang C,Li N,Milagres T,Kuwabara M,Wempe MF,Rabinowitz JD,Johnson RJ,Tolan DR

Fri Jun 01 00:00:00 EDT 2018

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

Journal of translational medicine

Han Q,Ma Y,Wang H,Dai Y,Chen C,Liu Y,Jing L,Sun X

Fri Jul 20 00:00:00 EDT 2018

Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis.

Journal of translational medicine

Han Q,Ma Y,Wang H,Dai Y,Chen C,Liu Y,Jing L,Sun X

Fri Jul 20 00:00:00 EDT 2018

Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase.

Nature medicine

Hunter RW,Hughey CC,Lantier L,Sundelin EI,Peggie M,Zeqiraj E,Sicheri F,Jessen N,Wasserman DH,Sakamoto K

Sat Sep 01 00:00:00 EDT 2018

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Cell metabolism

Thwe PM,Pelgrom LR,Cooper R,Beauchamp S,Reisz JA,D'Alessandro A,Everts B,Amiel E

Tue Sep 05 00:00:00 EDT 2017

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Cell metabolism

Thwe PM,Pelgrom LR,Cooper R,Beauchamp S,Reisz JA,D'Alessandro A,Everts B,Amiel E

Tue Sep 05 00:00:00 EDT 2017

Cell-Intrinsic Glycogen Metabolism Supports Early Glycolytic Reprogramming Required for Dendritic Cell Immune Responses.

Cell metabolism

Thwe PM,Pelgrom LR,Cooper R,Beauchamp S,Reisz JA,D'Alessandro A,Everts B,Amiel E

Tue Sep 05 00:00:00 EDT 2017

1,2-Dichloroethane impairs glucose and lipid homeostasis in the livers of NIH Swiss mice.

Toxicology

Wang T,Xu D,Fan Q,Rong W,Zheng J,Gao C,Li G,Zeng N,Guo T,Zeng L,Wang F,Xiao C,Cai L,Tang S,Deng X,Yin X,Huang M,Lu F,Hu Q,Chen W,Huang Z,Wang Q

Sat Apr 01 00:00:00 EDT 2017

1,2-Dichloroethane impairs glucose and lipid homeostasis in the livers of NIH Swiss mice.

Toxicology

Wang T,Xu D,Fan Q,Rong W,Zheng J,Gao C,Li G,Zeng N,Guo T,Zeng L,Wang F,Xiao C,Cai L,Tang S,Deng X,Yin X,Huang M,Lu F,Hu Q,Chen W,Huang Z,Wang Q

Sat Apr 01 00:00:00 EDT 2017

Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.

Bioscience reports

Yang R,Zhang M,Gustafson AR,Wang E,Cole MP,Tooley CE,Cheng A

Fri May 01 00:00:00 EDT 2015

Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.

Bioscience reports

Yang R,Zhang M,Gustafson AR,Wang E,Cole MP,Tooley CE,Cheng A

Fri May 01 00:00:00 EDT 2015

Induction of protective immunity against Chlamydia muridarum intravaginal infection with a chlamydial glycogen phosphorylase.

PloS one

Li Z,Lu C,Peng B,Zeng H,Zhou Z,Wu Y,Zhong G

Mon Aug 20 00:00:00 EDT 2012

Induction of protective immunity against Chlamydia muridarum intravaginal infection with a chlamydial glycogen phosphorylase.

PloS one

Li Z,Lu C,Peng B,Zeng H,Zhou Z,Wu Y,Zhong G

Mon Aug 20 00:00:00 EDT 2012

20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.

Molecular endocrinology (Baltimore, Md.)

Lai G,Wu J,Liu X,Zhao Y

Thu Nov 01 00:00:00 EDT 2012

20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.

Molecular endocrinology (Baltimore, Md.)

Lai G,Wu J,Liu X,Zhao Y

Thu Nov 01 00:00:00 EDT 2012

20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.

Molecular endocrinology (Baltimore, Md.)

Lai G,Wu J,Liu X,Zhao Y

Thu Nov 01 00:00:00 EDT 2012

20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.

Molecular endocrinology (Baltimore, Md.)

Lai G,Wu J,Liu X,Zhao Y

Thu Nov 01 00:00:00 EDT 2012

Liver-specific inducible nitric-oxide synthase expression is sufficient to cause hepatic insulin resistance and mild hyperglycemia in mice.

The Journal of biological chemistry

Shinozaki S,Choi CS,Shimizu N,Yamada M,Kim M,Zhang T,Shiota G,Dong HH,Kim YB,Kaneki M

Fri Oct 07 00:00:00 EDT 2011

Liver-specific inducible nitric-oxide synthase expression is sufficient to cause hepatic insulin resistance and mild hyperglycemia in mice.

The Journal of biological chemistry

Shinozaki S,Choi CS,Shimizu N,Yamada M,Kim M,Zhang T,Shiota G,Dong HH,Kim YB,Kaneki M

Fri Oct 07 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: glycogen liver; Glycogen phosphorylase, liver form; GPLL; liver glycogen phosphorylase

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Gene Aliases: GSD6; Lgp; PYGL

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UniProt ID: (Human) P06737, (Mouse) Q9ET01, (Rat) P09811

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Entrez Gene ID: (Human) 5836, (Mouse) 110095, (Rat) 64035

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Function(s)

purine nucleobase binding protein binding ATP binding glucose binding drug binding glycogen phosphorylase activity AMP binding vitamin binding pyridoxal phosphate binding bile acid binding protein homodimerization activity nucleotide binding catalytic activity phosphorylase activity transferase activity transferase activity, transferring glycosyl groups carbohydrate binding glycosyltransferase transferase metabolite interconversion enzyme

Process(es)

glycogen metabolic process glycogen catabolic process 5-phosphoribose 1-diphosphate biosynthetic process glucose homeostasis necroptotic process carbohydrate metabolic process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PYGL Polyclonal Antibody

PYGL Antibody (15851-1-AP) in ICC/IF

Product Details

15851-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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