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PYGL Antibody (A304-361A) in WB
Detection of human and mouse PYGL by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PYGL antibody A304-361A (lot A304-361A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A304-361A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 797 to 847 of Human Phosphorylase, Glycogen, Liver.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glycogen liver; Glycogen phosphorylase, liver form; GPLL
Gene Aliases: GSD6; PYGL
UniProt ID: (Human) P06737, (Mouse) Q9ET01
Entrez Gene ID: (Human) 5836, (Mouse) 110095
purine nucleobase binding
protein binding
ATP binding
glucose binding
drug binding
glycogen phosphorylase activity
AMP binding
vitamin binding
pyridoxal phosphate binding
bile acid binding
protein homodimerization activity
nucleotide binding
catalytic activity
phosphorylase activity
transferase activity
transferase activity, transferring glycosyl groups
carbohydrate binding
glycosyltransferase
transferase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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