Invitrogen

Pan Cytokeratin Monoclonal Antibody (AE1/AE3), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

57 References

View all (71) Cytokeratin Pan Type I/II antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Pan Cytokeratin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 25

Pan Cytokeratin Antibody (53-9003-82) in ICC/IF

Immunocytochemistry of fixed and permeabilized MCF-7 cells using 1 µg/mL of Mouse IgG1 K Isotype Control Alexa Fluor® 488 (left) or 1 µg/mL of Anti-Pan Cytokeratin (AE1/AE3) Alexa Fluor® 488 (right). Nuclei are stained with DAPI.

Pan Cytokeratin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pan Cytokeratin Antibody in Western Blot (WB)

Pan Cytokeratin Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

Pan Cytokeratin Antibody in Immunohistochemistry (IHC)

Pan Cytokeratin Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

53-9003-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 19 publications 19 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 19 publications 19 publications

Flow Cytometry (Flow)

View 8 publications 8 publications

in situ PLA (PLA)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rabbit, Rhesus monkey

Published species

Human, Mouse, Pig, Rabbit

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AE1/AE3

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834350

Product Specific Information

Description: The monoclonal antibodies AE1 and AE3 recognize many of the acidic and basic cytokeratin family members. Cytokeratins are intermediate filament proteins comprising one component of the cytoskeleton. There are two large families of cytokeratins, acidic and basic, but all contain the same basic domains (i.e. an alpha-helical core with an N- and C-terminal domain). The proteins are expressed in epithelial cells, but are developmentally regulated. Many tumors also express these proteins and their expression can help identify the origin of a neoplasm.

The AE3 monoclonal antibody recognizes the 65 to 67 triplet, 64, 59, 58, 56, 54 and 52kD proteins also known as cytokeratin 1, 2, 3, 4, 5, 6, 7, and 8 while the AE1 antibody recognizes 56.5, 54', 50, 50', 48, and 40 kDa proteins (also known as CK10, 14, 15, 16 and 19). These antibodies can be used on a wide array of tissue samples from mouse, human, rat, primates (cynomolgus and rhesus), dog, cat, rabbit, and chicken.

Applications Reported: This AE1/AE3 antibody has been reported for use in immunohistochemical staining, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This AE1/AE3 antibody has been tested by immunofluorescent staining of formaldehyde-fixed and permeabilized or methanol-fixed MCF7 cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Perineural invasion predicts poor survival and cervical lymph node metastasis in oral squamous cell carcinoma.

Medicina oral, patologia oral y cirugia bucal

Martínez-Flores R,Gómez-Soto B,Lozano-Burgos C,Niklander SE,Lopes MA,González-Arriagada WA

53-9003-82 was used in Immunohistochemistry to suggest further investigations to test the combined PNI-TB scoring system in risk stratification models for OSCC.

Fri Sep 01 00:00:00 EDT 2023

Single cell transcriptomic analyses implicate an immunosuppressive tumor microenvironment in pancreatic cancer liver metastasis.

Nature communications

Zhang S,Fang W,Zhou S,Zhu D,Chen R,Gao X,Li Z,Fu Y,Zhang Y,Yang F,Zhao J,Wu H,Wang P,Shen Y,Shen S,Xu G,Wang L,Yan C,Zou X,Chen D,Lv Y

53-9003-82 was used in Immunohistochemistry-immunofluorescence to present the single-cell transcriptomic landscape of synchronously resected pancreatic ductal adenocarcinoma primary tumors and matched liver metastases.

Wed Aug 23 00:00:00 EDT 2023

Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits.

Communications biology

Rodriguez E,Boelaars K,Brown K,Madunić K,van Ee T,Dijk F,Verheij J,Li RJE,Schetters STT,Meijer LL,Le Large TYS,Driehuis E,Clevers H,Bruijns SCM,O'Toole T,van Vliet SJ,Bijlsma MF,Wuhrer M,Kazemier G,Giovannetti E,Garcia-Vallejo JJ,van Kooyk Y

53-9003-82 was used in Flow cytometry/Cell sorting to show mechanisms by which the glyco-code contributes to the tolerogenic microenvironment in PDAC.

Tue Jan 11 00:00:00 EST 2022

Spatial meta-transcriptomics reveal associations of intratumor bacteria burden with lung cancer cells showing a distinct oncogenic signature.

Journal for immunotherapy of cancer

Wong-Rolle A,Dong Q,Zhu Y,Divakar P,Hor JL,Kedei N,Wong M,Tillo D,Conner EA,Rajan A,Schrump DS,Jin C,Germain RN,Zhao C

53-9003-82 was used in Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to develop a novel spatial meta-transcriptomic method simultaneously detecting the expression level of 1,811 host genes and 3 microbe targets (bacteria, fungi, and cytomegalovirus).

Fri Jul 01 00:00:00 EDT 2022

miR-126-3p-loaded small extracellular vesicles secreted by urine-derived stem cells released from a phototriggered imine crosslink hydrogel could enhance vaginal epithelization after vaginoplasty.

Stem cell research & therapy

Xu Y,Qiu Y,Lin Q,Huang C,Li J,Chen L,Xue Z,Wu Q,Wang Y

53-9003-82 was used in Immunohistochemistry to indicate that piGEL-sEVs could be a novel promising approach for enhancing the epithelization of the neovagina after vaginoplasty and provided useful data for understanding the underlying mechanism of the effect of hUSC-sEVs on epithelization.

Sat Jul 23 00:00:00 EDT 2022

Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps.

Cell

Chen B,Scurrah CR,McKinley ET,Simmons AJ,Ramirez-Solano MA,Zhu X,Markham NO,Heiser CN,Vega PN,Rolong A,Kim H,Sheng Q,Drewes JL,Zhou Y,Southard-Smith AN,Xu Y,Ro J,Jones AL,Revetta F,Berry LD,Niitsu H,Islam M,Pelka K,Hofree M,Chen JH,Sarkizova S,Ng K,Giannakis M,Boland GM,Aguirre AJ,Anderson AC,Rozenblatt-Rosen O,Regev A,Hacohen N,Kawasaki K,Sato T,Goettel JA,Grady WM,Zheng W,Washington MK,Cai Q,Sears CL,Goldenring JR,Franklin JL,Su T,Huh WJ,Vandekar S,Roland JT,Liu Q,Coffey RJ,Shrubsole MJ,Lau KS

53-9003-82 was used in Flow cytometry/Cell sorting to present a single-cell transcriptomic and imaging atlas of the two most common human colorectal polyps, conventional adenomas and serrated polyps, and their resulting CRC counterparts.

Wed Dec 22 00:00:00 EST 2021

TALPID3/KIAA0586 Regulates Multiple Aspects of Neuromuscular Patterning During Gastrointestinal Development in Animal Models and Human.

Frontiers in molecular neuroscience

Delalande JM,Nagy N,McCann CJ,Natarajan D,Cooper JE,Carreno G,Dora D,Campbell A,Laurent N,Kemos P,Thomas S,Alby C,Attié-Bitach T,Lyonnet S,Logan MP,Goldstein AM,Davey MG,Hofstra RMW,Thapar N,Burns AJ

Published figure using Pan Cytokeratin monoclonal antibody (Product # 53-9003-82) in Immunohistochemistry (Frozen)

Sat Jul 16 00:00:00 EDT 2022

TALPID3/KIAA0586 Regulates Multiple Aspects of Neuromuscular Patterning During Gastrointestinal Development in Animal Models and Human.

Frontiers in molecular neuroscience

Published figure using Pan Cytokeratin monoclonal antibody (Product # 53-9003-82) in Immunohistochemistry (Frozen)

Sat Jul 16 00:00:00 EDT 2022

Prognostic impact of CD73 expression and its relationship to PD-L1 in patients with radically treated pancreatic cancer.

Virchows Archiv : an international journal of pathology

Tahkola K,Ahtiainen M,Kellokumpu I,Mecklin JP,Laukkarinen J,Laakkonen J,Kenessey I,Jalkanen S,Salmi M,Böhm J

53-9003 was used in Immunohistochemistry to investigate the prognostic significance of CD73 in PDAC.

Mon Feb 01 00:00:00 EST 2021

Prognostic impact of CD73 expression and its relationship to PD-L1 in patients with radically treated pancreatic cancer.

Virchows Archiv : an international journal of pathology

Tahkola K,Ahtiainen M,Kellokumpu I,Mecklin JP,Laukkarinen J,Laakkonen J,Kenessey I,Jalkanen S,Salmi M,Böhm J

53-9003 was used in Immunohistochemistry to investigate the prognostic significance of CD73 in PDAC.

Mon Feb 01 00:00:00 EST 2021

Clinical Significance of TWIST-Positive Circulating Tumor Cells in Patients with Esophageal Squamous Cell Carcinoma.

Gut and liver

Lee HJ,Kim GH,Park SJ,Kwon CH,Lee MW,Lee BE,Baek DH,I H

Published figure using Pan Cytokeratin monoclonal antibody (Product # 53-9003-82) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

Clinical Significance of TWIST-Positive Circulating Tumor Cells in Patients with Esophageal Squamous Cell Carcinoma.

Gut and liver

Lee HJ,Kim GH,Park SJ,Kwon CH,Lee MW,Lee BE,Baek DH,I H

Published figure using Pan Cytokeratin monoclonal antibody (Product # 53-9003-82) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

Functional cooperation between co-amplified genes promotes aggressive phenotypes of HER2-positive breast cancer.

Cell reports