Bioss

Pan Cytokeratin/p-CK Polyclonal Antibody

View all (39) Cytokeratin Pan Type I antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)

Pan Cytokeratin/p-CK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

Pan Cytokeratin/p-CK Antibody (BS-10403R) in IHC (P)

Paraformaldehyde-fixed, paraffin embedded mouse lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Pan cytokeratin Polyclonal Antibody, Unconjugated (bs-10403R) at 1:400 overnight at 4°C, followed by a conjugated secon... View More

Product Image Gallery

Product Details

BS-10403R

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100-1:500

ELISA (ELISA)

1:500-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide derived from human cytokeratin 16.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Cytokeratin pan is part of a subfamily of intermediate filament proteins that are characterized by remarkable biochemical diversity, and represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight between 40 kDa- 68 kDa, and an isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. The composition of cytokeratin pairs vary with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: 40-kDa keratin intermediate filament; 56 kDa cytokeratin; Acidic Cytokeratin; Cell proliferation-inducing gene 46 protein; CK-10; CK-14; CK-15; CK-16; CK-18; CK-19; CK-9; cytokeratin 10; cytokeratin 14; cytokeratin 15; cytokeratin 16; cytokeratin 18; cytokeratin 19; cytokeratin 9; Cytokeratin endo B; Cytokeratin-10; Cytokeratin-14; Cytokeratin-15; Cytokeratin-16; Cytokeratin-18; Cytokeratin-19; Cytokeratin-9; Endo B; epidermal keratin 10; epidermolysis bullosa simplex, Dowling-Meara, Koebner; focal non-epidermolytic palmoplantar keratoderma; HMWCK; intermediate filament protein; K10; K14; K15; K16; K18; K19; K9; keratin 10, type I; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; keratin 15, type I; keratin 16, type I; keratin 18, type I; keratin 19, type I; keratin 9 (epidermolytic palmoplantar keratoderma); keratin 9, type I; keratin complex 1, acidic, gene 10; keratin complex 1, acidic, gene 14; keratin complex 1, acidic, gene 15; keratin complex 1, acidic, gene 16; keratin complex 1, acidic, gene 18; keratin complex 1, acidic, gene 19; keratin complex 1, acidic, gene 9; Keratin D; keratin intermediate filament 16a; keratin intermediate filament 16b; Keratin, type I cytoskeletal 10; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 15; Keratin, type I cytoskeletal 16; Keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 59 kDa; Keratin, type I cytoskeletal 9; keratin, type I, 40-kd; Keratin-10; Keratin-14; Keratin-15; keratin-15, basic; keratin-15, beta; Keratin-16; Keratin-18; Keratin-19; Keratin-9; krt10 {ECO:0000250|UniProtKB:P13645}; krt15 {ECO:0000250|UniProtKB:P19012}; krt18 {ECO:0000250|UniProtKB:P05783}; krt9; pan keratin; pankeratin; Spermatid perinuclear ring manchette protein K9; suprabasal cytokeratin 10; type I cytoskeletal 15; type I cytoskeletal 9; type I keratin KA10; Type I keratin Ka14; Type I keratin Ka15; type I keratin KA16; Type I keratin Ka19

View more View less

Gene Aliases: AI324768; AI528832; AI626930; AI663979; BCIE; BIE; CK-18; CK-9; CK10; CK14; CK15; CK16; CK18; CK19; CYK18; D130054E02Rik; EBS3; EBS4; EHK; EndoC; EPPK; FNEPPK; K10; K14; K15; K16; K18; K19; K1C1; K1CO; K1CP; K1CS; K9; Ka10; Ka14; Ka15; Ka16; Ka19; Kerd; KPP; Krt-1.10; Krt-1.14; Krt-1.19; Krt1-10; Krt1-14; Krt1-15; Krt1-16; Krt1-18; Krt1-19; Krt1-9; KRT10; KRT14; KRT15; KRT16; KRT16A; KRT18; KRT19; KRT9; KRTB; NEPPK; NFJ; PC1; PIG46