Invitrogen

Pan Ras Monoclonal Antibody (Ras10)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

14 References

View all (15) Pan Ras antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Pan Ras Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

Pan Ras Antibody (MA1-012) in ICC/IF

Immunofluorescent analysis of Ras (green) in U20S cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a mouse monoclonal antibody recognizing Ras (Product # MA1-012), at a dilution of 1:100 for at least 1 hour at room temperature. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-mouse secondary antibody (Product # 35502) at a ... View More

Pan Ras Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pan Ras Antibody in Immunoprecipitation (IP)

Pan Ras Antibody in Western Blot, Immunoprecipitation (WB, IP)

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Product Details

MA1-012

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

Ras10

Immunogen

Purified recombinant Ras protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536664

Product Specific Information

This antibody recognizes normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families.

Target Information

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PD-1 blockade delays tumor growth by inhibiting an intrinsic SHP2/Ras/MAPK signalling in thyroid cancer cells.

Journal of experimental & clinical cancer research : CR

Liotti F,Kumar N,Prevete N,Marotta M,Sorriento D,Ieranò C,Ronchi A,Marino FZ,Moretti S,Colella R,Puxeddu E,Paladino S,Kano Y,Ohh M,Scala S,Melillo RM

MA1-012 was used in Immunoprecipitation to analyse expression and functions of PD-1 in thyroid cancer (TC).

Thu Jan 07 00:00:00 EST 2021

PD-1 blockade delays tumor growth by inhibiting an intrinsic SHP2/Ras/MAPK signalling in thyroid cancer cells.

Journal of experimental & clinical cancer research : CR

Liotti F,Kumar N,Prevete N,Marotta M,Sorriento D,Ieranò C,Ronchi A,Marino FZ,Moretti S,Colella R,Puxeddu E,Paladino S,Kano Y,Ohh M,Scala S,Melillo RM

MA1-012 was used in Immunoprecipitation to analyse expression and functions of PD-1 in thyroid cancer (TC).

Thu Jan 07 00:00:00 EST 2021

Evaluating the Epithelial-Mesenchymal Program in Human Breast Epithelial Cells Cultured in Soft Agar Using a Novel Macromolecule Extraction Protocol.

Cancers

Lau HY,Tang J,Casey PJ,Wang M

MA1-012 was used in Western Blotting to investigate the role of epithelial-mesenchymal transition in human mammary epithelial cell colony formation inagar.

Mon Feb 15 00:00:00 EST 2021

Evaluating the Epithelial-Mesenchymal Program in Human Breast Epithelial Cells Cultured in Soft Agar Using a Novel Macromolecule Extraction Protocol.

Cancers

Lau HY,Tang J,Casey PJ,Wang M

MA1-012 was used in Western Blotting to investigate the role of epithelial-mesenchymal transition in human mammary epithelial cell colony formation inagar.

Mon Feb 15 00:00:00 EST 2021

Pharmacokinetic and Pharmacodynamic Factors Contribute to Synergism between Let-7c-5p and 5-Fluorouracil in Inhibiting Hepatocellular Carcinoma Cell Viability.

Drug metabolism and disposition: the biological fate of chemicals

Jilek JL,Tu MJ,Zhang C,Yu AM

MA1-012 was used in Western Blot to identify small-molecule drugs that may synergistically act with the bioengineered microRNA let-7c against hepatocellular carcinoma.

Tue Dec 01 00:00:00 EST 2020

Lipophilic statins limit cancer cell growth and survival, via involvement of Akt signaling.

PloS one

Beckwitt CH,Shiraha K,Wells A

Mon Nov 26 00:00:00 EST 2018

PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Strazza M,Azoulay-Alfaguter I,Peled M,Smrcka AV,Skolnik EY,Srivastava S,Mor A

MA1012 was used in western blot to discover new regulatory proteins involved in Rap1-dependent T-cell adhesion and migration.

Tue Mar 07 00:00:00 EST 2017

Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms.

Oncogene

Lau HY,Tang J,Casey PJ,Wang M

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Western Blot

Thu Jul 06 00:00:00 EDT 2017

Pallidin protein in neurodevelopment and its relation to the pathogenesis of schizophrenia.

Molecular medicine reports

Shi Q,Li C,Li K,Liu Q

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Western Blot

Wed Feb 01 00:00:00 EST 2017

Calcium-dependent binding of Myc to calmodulin.

Oncotarget

Raffeiner P,Schraffl A,Schwarz T,Röck R,Ledolter K,Hartl M,Konrat R,Stefan E,Bister K

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Western Blot

Tue Jan 10 00:00:00 EST 2017

Aurora kinase A interacts with H-Ras and potentiates Ras-MAPK signaling.

Oncotarget

Umstead M,Xiong J,Qi Q,Du Y,Fu H

Published figure using Pan Ras monoclonal antibody (Product # MA1-012) in Immunoprecipitation

Tue Apr 25 00:00:00 EDT 2017

Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation.

The Journal of biological chemistry

Mitra S,Ghosh B,Gayen N,Roy J,Mandal AK

MA1012 was used in western blot to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase

Fri Nov 18 00:00:00 EST 2016

Inhibition of RAS activation due to a homozygous ezrin variant in patients with profound intellectual disability.

Human mutation

Riecken LB,Tawamie H,Dornblut C,Buchert R,Ismayel A,Schulz A,Schumacher J,Sticht H,Pohl KJ,Cui Y,Reis A,Morrison H,Abou Jamra R

MA1-012 was used in western blot to report that ezrin is required for Ras activation.

Sun Feb 01 00:00:00 EST 2015

An improved isoprenylcysteine carboxylmethyltransferase inhibitor induces cancer cell death and attenuates tumor growth in vivo.

Cancer biology & therapy

Lau HY,Ramanujulu PM,Guo D,Yang T,Wirawan M,Casey PJ,Go ML,Wang M

MA1-012 was used in western blot to show that 8.12 is a pharmacological inhibitor of Icmt

Mon Sep 01 00:00:00 EDT 2014

Production and characterization of anti-RAS p21 monoclonal antibodies.

Hybridoma

Hamer PJ,Trimpe KL,Pullano T,Ng S,LaVecchio JA,Petit DA,DeLellis R,Wolfe H,Carney WP

MA1-012 was used in [flow cytometry] to characterize the antibody's reactivity to normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families.

Sat Dec 01 00:00:00 EST 1990

Bioinformatics

Protein Aliases: c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-K-ras; c-Ki-ras; c-Kirsten-ras protein; c-ras-Ki-2 activated oncogene; cellular c-Ki-ras2; cellular c-Ki-ras2 proto-oncogene; GTP- and GDP-binding peptide B; GTPase HRas; GTPase Hras1; GTPase KRas; GTPase NRas; H-ras 1 protein; H-Ras-1; Ha-Ras; Ha-Ras1 proto-oncoprotein; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; K RAS; K-Ras 2; K-ras p21 protein; Ki-Ras; Kirsten rat sarcoma oncogene 2, expressed; Kirsten rat sarcoma viral oncogene homolog; N-ras protein part 4; Neuroblastoma RAS; neuroblastoma RAS viral (v-ras) oncogene homolog; oncogene KRAS2; p19 H-RasIDX protein; p21 protein; p21ras; PR310 c-K-ras oncogene; Ras family small GTP binding protein H-Ras; RASK; RASN; transformation gene: oncogene HAMSV; Transforming protein N-Ras; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v-ras neuroblastoma RAS viral oncogene homolog

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Gene Aliases: AI929937; ALPS4; AV095280; C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; C-K-RAS; c-rasHa; CFC2; CMNS; CTLO; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; K-ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS; Kras-2; KRAS1; KRAS2; N-ras; NCMS; NRAS; NRAS1; NS; NS3; NS6; p21B; p21ras; RALD; ras; RASH1; RASK2

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UniProt ID: (Human) P01112, (Mouse) Q61411, (Human) P01116, (Mouse) P32883, (Human) P01111

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Entrez Gene ID: (Human) 3265, (Mouse) 15461, (Human) 3845, (Mouse) 16653, (Human) 4893, (Mouse) 18176

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Function(s)

GTPase activity protein binding GTP binding protein C-terminus binding nucleotide binding GMP binding GDP binding LRR domain binding protein complex binding small GTPase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Pan Ras Monoclonal Antibody (Ras10)

Pan Ras Antibody (MA1-012) in ICC/IF

Product Details

MA1-012

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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