Invitrogen

Pan-cadherin Polyclonal Antibody

2 Published Figures

15 References

View all (16) Pan-cadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (8)
Published Figures (2)

Pan-cadherin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

Pan-cadherin Antibody (71-7100) in ICC/IF

Immunofluorescence analysis of Pan-Cadherin was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cadherin pan Rabbit Polyclonal Antibody (Product # 71-7100) at 2 µg/mL in 0.1% BSA and incubated for 3 hours... View More

Pan-cadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Pan-cadherin Antibody in Western Blot (WB)

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Product Details

71-7100

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2-3 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse, Non-human primate, Rat, Xenopus

Published species

Bovine, Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the carboxy-terminus of the chicken N-cadherin protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533992

Product Specific Information

This peptide antibody is broadly cross-reactive with all members of the cadherin family of proteins including N-cadherin, E-cadherin, P-cadherin, and R-cadherin. The antibody also displays broad species cross-reactivity including human, bovine, mouse, rat, chicken, amphibian, as well as other species. Rabbit anti-pan-Cadherin is useful as both a ubiquitous cadherin probe as well as a marker for adherens junctions.

71-7100 was used successfully in the immunofluorescence analysis of pan cadherin in MDCK cells.

Target Information

Pan Cadherin including CDH1, CDH2, CDH3, CDH4 protein belong to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peptide functionalized DNA hydrogel enhances neuroblastoma cell growth and differentiation.

Nanoscale

Hivare P,Gangrade A,Swarup G,Bhavsar K,Singh A,Gupta R,Thareja P,Gupta S,Bhatia D

71-7100 was used in Immunocytochemistry-immunoflourescence to demonstrate strongly the ability of DNA-peptide based scaffolds as potential materials to develop nerve tissue conduits for neural tissue engineering applications in the future.

Thu Jun 23 00:00:00 EDT 2022

Application of Three-Dimensional Culture Method in the Cardiac Conduction System Research.

Methods and protocols

Mishra A,Pasumarthi KBS

71-7100 was used in Western Blotting to describe a new and reproducible 3-D cell culture method for studying cardiac cell lineage differentiation in vitro.

Tue Jun 14 00:00:00 EDT 2022

A CACNA1C variant associated with cardiac arrhythmias provides mechanistic insights in the calmodulation of L-type Ca²⁺ channels.

The Journal of biological chemistry

Zhao J,Segura E,Marsolais M,Parent L

71-7100 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to conclude that the CACNA1C clinical variant mimics the increased activity associated with the upregulation of CaV1.2 by Ca2+-CaM, thus maintaining a majority of channels in a constitutively active mode that could ultimately promote ventricular arrhythmias.

Thu Dec 01 00:00:00 EST 2022

Soluble RARRES1 induces podocyte apoptosis to promote glomerular disease progression.

The Journal of clinical investigation

Chen A,Feng Y,Lai H,Ju W,Li Z,Li Y,Wang A,Hong Q,Zhong F,Wei C,Fu J,Guan T,Liu B,Kretzler M,Lee K,He JC

71-7100 was used in Western Blotting to demonstrates an important role and the mechanism of RARRES1 in podocyte injury in glomerular disease.

Thu Oct 01 00:00:00 EDT 2020

Lipidomics unveils lipid dyshomeostasis and low circulating plasmalogens as biomarkers in a monogenic mitochondrial disorder.

JCI insight

Ruiz M,Cuillerier A,Daneault C,Deschênes S,Frayne IR,Bouchard B,Forest A,Legault JT,Vaz FM,Rioux JD,Burelle Y,Des Rosiers C

71-7100 was used in Western Blotting to show the value of lipidomics to unveil unexpected mechanisms underlying lipid dyshomeostasis ensuing from mitochondrial dysfunction, implying peroxisomes and liver which likely contribute to the pathophysiology of Leigh syndrome French-Canadian variant.

Thu Jul 25 00:00:00 EDT 2019

Enteropathogenic E. coli effectors EspF and Map independently disrupt tight junctions through distinct mechanisms involving transcriptional and post-transcriptional regulation.

Scientific reports

Singh AP,Sharma S,Pagarware K,Siraji RA,Ansari I,Mandal A,Walling P,Aijaz S

71-7100 was used in Western Blotting to provide evidence for the distinct roles of Map and EspF in tight junction disruption through non-synergistic functions.

Tue Feb 27 00:00:00 EST 2018

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion.

International journal of nanomedicine

Wierzbicki M,Jaworski S,Kutwin M,Grodzik M,Strojny B,Kurantowicz N,Zdunek K,Chodun R,Chwalibog A,Sawosz E

Published figure using Pan-cadherin polyclonal antibody (Product # 71-7100) in Western Blot

Fri Jan 26 00:00:00 EST 2018

Different responses in transformation of MDCK cells in 2D and 3D culture by v-Src as revealed by microarray techniques, RT-PCR and functional assays.

Laboratory investigation; a journal of technical methods and pathology

Töyli M,Rosberg-Kulha L,Capra J,Vuoristo J,Eskelinen S

71-7100 was used in immunocytochemistry and western blot to study the differentiation and transformation of canine kidney MDCK cells cultured in either a 2D or 3D environment

Tue Jun 01 00:00:00 EDT 2010

Immunofluorescent analysis of connexin-43 using monoclonal antibodies to its extracellular domain.

Bulletin of experimental biology and medicine

Baklaushev VP,Gurina OI,Yusubalieva GM,Grinenko NF,Cytrin EB,Victorov IV,Chekhonin VP

71-7100 was used in immunohistochemistry - frozen section to assess the use of connexin-43 monoclonal antibodies

Thu Oct 01 00:00:00 EDT 2009

Proteomic Analysis of Vascular Endothelial Cells-Effects of Laminar Shear Stress and High Glucose.

Journal of proteomics & bioinformatics

Wang XL,Fu A,Spiro C,Lee HC

71-7100 was used in western blot to investigate the effects of glucose and shear stress using bovine aortic endothelial cells

Thu Jan 01 00:00:00 EST 2009

p27Kip1 is expressed in proliferating cells in its form phosphorylated on threonine 187.

BMC clinical pathology

Troncone G,Martinez JC,Iaccarino A,Zeppa P,Caleo A,Russo M,Migliaccio I,Motti ML,Califano D,Palmieri EA,Palombini L

71-7100 was used in immunohistochemistry - paraffin section and western blot to demonstrate that p27 is expressed in proliferating cells

Wed Feb 23 00:00:00 EST 2005

Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis.

Cancer research

Croft DR,Sahai E,Mavria G,Li S,Tsai J,Lee WM,Marshall CJ,Olson MF

Wed Dec 15 00:00:00 EST 2004

Conditional ROCK activation in vivo induces tumor cell dissemination and angiogenesis.

Cancer research

Croft DR,Sahai E,Mavria G,Li S,Tsai J,Lee WM,Marshall CJ,Olson MF

Wed Dec 15 00:00:00 EST 2004

ESX induces transformation and functional epithelial to mesenchymal transition in MCF-12A mammary epithelial cells.

Oncogene

Schedin PJ,Eckel-Mahan KL,McDaniel SM,Prescott JD,Brodsky KS,Tentler JJ,Gutierrez-Hartmann A

Thu Mar 04 00:00:00 EST 2004

Differential regulation of the TRAIL death receptors DR4 and DR5 by the signal recognition particle.

Molecular biology of the cell

Ren YG,Wagner KW,Knee DA,Aza-Blanc P,Nasoff M,Deveraux QL

Mon Nov 01 00:00:00 EST 2004

Myofibroblast development is characterized by specific cell-cell adherens junctions.

Molecular biology of the cell

Hinz B,Pittet P,Smith-Clerc J,Chaponnier C,Meister JJ

Wed Sep 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: ARC-1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); cadherin 15, M-cadherin (myotubule); cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); cadherin 3, type 1, P-cadherin (placental); cadherin 4 type 1 R-cadherin (retinal); cadherin 4, type 1, preproprotein; cadherin 4, type 1, R-cadherin (retinal); cadherin e; Cadherin-1; Cadherin-2; Cadherin-3; Cadherin-4; cadherin-E; calcium-dependent adhesion protein, epithelial; calcium-dependent adhesion protein, neuronal; calcium-dependent adhesion protein, placental; CAM 120/80; CD324; CD325; CDH1; CDw325; CDw325 antigen; cell-CAM 120/80; E-Cadherin; E-cadherin 1; Epithelial cadherin; L-CAM; Liver cell adhesion molecule; liver cell adhesion protein; Maternally-expressed neural cadherin; N-cadherin; N-cadherin 1; Neural cadherin; neural calcium-dependent cell adhesion molecule; P-cadherin; Pan Cadherin; Placental cadherin; Placental-cadherin; R-CAD; R-cadherin; retina cadherin; Retinal cadherin; RPE-specific cadherin; unnamed protein product; Uvomorulin; XmN-cadherin; xTCAD-1

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Gene Aliases: AA960649; AI385538; Arc-1; AW120700; CAD4; Cadherin-1; cadherin-2; cadherin-4; Cadp; CD324; CD325; CDH1; CDH2; CDH3; CDH4; Cdh4l; CDHE; CDHN; CDHP; CDw325; E-cad; ECAD; HJMD; K-CAM; L-CAM; LCAM; N-cadherin; NCAD; P-cadherin; PCAD; R-CAD; R-Cadh; R-cadherin; RCAD; Um; UVO; Uvomorulin; XELAEV_18024918mg; XELAEV_18046239mg

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UniProt ID: (Chicken) P08641, (Dog) F1PAA9, (Human) P12830, (Chicken) P10288, (Human) P19022, (Human) P22223, (Chicken) P24503, (Human) P55283, (Mouse) P09803, (Rat) Q9R0T4, (Mouse) P15116, (Rat) Q9Z1Y3, (Mouse) P10287, (Mouse) P39038, (Xenopus) P79883, (Xenopus) P30944

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Function(s)

calcium ion binding protein binding cadherin binding beta-catenin binding ankyrin binding GTPase activating protein binding identical protein binding gamma-catenin binding glycoprotein binding cell adhesion molecule binding cadherin binding involved in cell-cell adhesion protein kinase binding protein phosphatase binding alpha-catenin binding extracellular matrix binding protein complex binding nitric-oxide synthase binding molecular_function protein domain specific binding metal ion binding enzyme binding cadherin

Process(es)

homophilic cell adhesion via plasma membrane adhesion molecules synapse assembly cell-cell adhesion via plasma-membrane adhesion molecules regulation of gene expression negative regulation of cell-cell adhesion negative regulation of cell migration adherens junction organization positive regulation of protein import into nucleus positive regulation of transcription, DNA-templated cellular response to lithium ion cellular response to indole-3-methanol protein localization to plasma membrane response to toxic substance single organismal cell-cell adhesion pituitary gland development extracellular matrix disassembly extracellular matrix organization neuron projection development response to drug positive regulation of transcription factor import into nucleus establishment of protein localization to plasma membrane cartilage condensation neural tube formation cell adhesion heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules brain development glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration neural crest cell delamination positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation regulation of synaptic transmission, glutamatergic radial glial cell differentiation limb development neuroepithelial cell differentiation trigeminal ganglion formation trigeminal ganglion structural organization regulation of oligodendrocyte progenitor proliferation negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance cell-cell adhesion cell aggregation regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering cerebral cortex development regulation of myelination regulation of Rho protein signal transduction positive regulation of muscle cell differentiation protein heterooligomerization retina homeostasis visual perception positive regulation of gene expression positive regulation of keratinocyte proliferation hair cycle process keratinization positive regulation of monophenol monooxygenase activity negative regulation of transforming growth factor beta2 production wound healing positive regulation of insulin-like growth factor receptor signaling pathway positive regulation of melanin biosynthetic process negative regulation of catagen canonical Wnt signaling pathway regulation of hair cycle by canonical Wnt signaling pathway positive regulation of melanosome transport axon guidance positive regulation of axon extension in utero embryonic development trophectodermal cell differentiation epithelial cell morphogenesis sensory perception of sound protein metabolic process regulation of protein localization regulation of water loss via skin regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation protein homooligomerization intestinal epithelial cell development salivary gland cavitation regulation of branching involved in salivary gland morphogenesis bicellular tight junction assembly cellular response to amino acid stimulus cochlea development regulation of protein localization to cell surface regulation of neuron migration response to organic substance regulation of signal transduction

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Pan-cadherin Polyclonal Antibody

Pan-cadherin Antibody (71-7100) in ICC/IF

Product Details

71-7100

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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