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FIGURE: 1 / 4
Parvalbumin Antibody (MA5-45927) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using undifferentiated SH-SY5Y cells. Samples were incubated with Parvalbumin monoclonal antibody (Product # MA5-45927) at 1:250, followed by AlexaFluor 488. Counterstain used was phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Parvalbumin antibody. (D) Ho... View More
Product Details
MA5-45927
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
C12
Immunogen
Synthetic peptide from the full length Rat Parvalbumin protein
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932381
Product Specific Information
A 1:500 dilution of MA5-45927 was sufficient for detection of Parvalbumin in 15 µg of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects 12 kDa.
Target Information
Parvalbumin (PV) is a calcium binding protein expressed in specific muscle fibers and fast-firing neurons. PV consists of a single, unbranched chain of linked amino acids and belongs to a larger group of EF hand proteins. Studies have demonstrated that parvalbumin acts in the decay of calcium in the contraction/ relaxation cycle of fast twitch muscles. This data has shown a positive correlation between the rate of relaxation and the concentration of parvalbumin. Parvalbumin is also expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. In amyotrophic lateral sclerosis (ALS) patents, parvalbumin immunoreactivity is specifically absent from neuron populations lost early in ALS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:9704; MGC116759; Parvalbumin (calcium binding protein); Parvalbumin alpha
Gene Aliases: D22S749; PALB1; Parv; PV; Pva; PVALB
UniProt ID: (Human) P20472, (Rat) P02625, (Mouse) P32848
Entrez Gene ID: (Human) 5816, (Rat) 25269, (Mouse) 19293
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