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NeoBiotechnologies
Perforin-1 (Pore Forming Protein) (Apoptosis Marker) Monoclonal Antibody (PRF1/2467)
FIGURE: 1 / 1
Perforin-1 (Pore Forming Protein) (Apoptosis Marker) Antibody (5551-MSM1-P1) in ARRAY
Analysis of Protein Array containing more than 19,000 full-length human proteins using Perforin-1 Mouse Monoclonal Antibody (PRF1/2467) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are descr... View More
Product Details
5551-MSM1-P1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
PRF1/2467
Immunogen
Recombinant human Perforin-1 protein fragment (around aa 413-552).
Conjugate
Form
Liquid
Concentration
200 µg/mL
Purification
Protein A/G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Antibody is stable for 24 months.
Positive Control: HDLM-2 cells. Human spleen tissue (IHC). Cellular Localization: Cytoplasmic
Specificity Comments: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytolysin; lymphocyte pore forming protein; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; PRF1 (pore forming protein 1); RP11-710A11.3
Gene Aliases: FLH2; HPLH2; P1; PFN1; PFP; PRF1
UniProt ID: (Human) P14222
Entrez Gene ID: (Human) 5551
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