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Invitrogen
Pericentrin Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Pericentrin Antibody (PA5-54109) in ICC/IF
Immunofluorescence analysis of Pericentrin was performed using MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Pericentrin Rabbit Polyclonal Antibody (Product # PA5-54109) at 4µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled... View More
Product Details
PA5-54109
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human Pericentrin. Recombinant protein control fragment (Product #RP-91791).
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2645392
Product Specific Information
Immunogen sequence: MLDLSSWSSP EVLRKDWTLE PWPSLPVTPH SGALSLCSAD TSLGDRADTS LPQTQGPGLL CSPGVSAAAL ALQWAESPPA DDHHVQRTAV EKDVEDFITT SFDSQETLSS PPPGLEGKAD RSEKSDGSG
Highest antigen sequence identity to the following orthologs: Mouse - 58%, Rat - 58%.
Target Information
This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70kD component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this gene product suggest that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Kendrin; Pericentrin; pericentrin-2; pericentrin-380; Pericentrin-B
Gene Aliases: KEN; KIAA0402; MOPD2; PCN; PCNT; PCNT2; PCNTB; PCTN2; SCKL4
UniProt ID: (Human) O95613
Entrez Gene ID: (Human) 5116
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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