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Periostin Monoclonal Antibody (1A11A3)

Periostin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Periostin Antibody (66491-1-IG) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue slide using (Product # 66491-1-Ig) Periostin antibody at dilution of 1:8,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Periostin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

66491-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2500-1:10,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:250-1:1,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:8,000

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A11A3

Immunogen

Periostin Fusion Protein Ag14487 (21-368 aa encoded by BC106710)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: ANNHYDKIL AHSRIRGRDQ GPNVCALQQI LGTKKKYFST CKNWYKKSIC GQKTTVLYEC CPGYMRMEGM KGCPAVLPID HVYGTLGIVG ATTTQRYSDA SKLREEIEGK GSFTYFAPSN EAWDNLDSDI RRGLESNVNV ELLNALHSHM INKRMLTKDL KNGMIIPSMY NNLGLFINHY PNGVVTVNCA RIIHGNQIAT NGVVHVIDRV LTQIGTSIQD FIEAEDDLSS FRAAAITSDI LEALGRDGHF TLFAPTNEAF EKLPRGVLER IMGDKVASEA LMKYHILNTL QCSESIMGGA VFETLEGNTI EIGCDGDSIT VNGIKMVNKK DIVTNNGVIH LIDQVLIPD (21-368 aa encoded by BC106710)

Target Information

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor beta 1(TGF beta 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SPARC in cancer-associated fibroblasts is an independent poor prognostic factor in non-metastatic triple-negative breast cancer and exhibits pro-tumor activity.

International journal of cancer

Alcaraz LB,Mallavialle A,Mollevi C,Boissière-Michot F,Mansouri H,Simony-Lafontaine J,Laurent-Matha V,Chardès T,Jacot W,Turtoi A,Roger P,Guiu S,Liaudet-Coopman E

Wed Mar 15 00:00:00 EDT 2023

Ciclopirox Inhibition of eIF5A Hypusination Attenuates Fibroblast Activation and Cardiac Fibrosis.

Journal of cardiovascular development and disease

Subbaiah KCV,Wu J,Tang WHW,Yao P

Sun Jan 29 00:00:00 EST 2023

Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhou T,Chen Y,Liao Z,Zhang L,Su D,Li Z,Yang X,Ke X,Liu H,Chen Y,Weng R,Shen H,Xu C,Wan Y,Xu R,Su P

Mon May 01 00:00:00 EDT 2023

Bioinformatics analysis and identification of dysregulated POSTN in the pathogenesis of keloid.

International wound journal

Xu H,Wang Z,Yang H,Zhu J,Hu Z

Mon May 01 00:00:00 EDT 2023

Micro/nano-topography promotes osteogenic differentiation of bone marrow stem cells by regulating periostin expression.

Colloids and surfaces. B, Biointerfaces

Li J,Hou W,Yang Y,Deng Q,Fu H,Yin Y,Duan K,Feng B,Guo T,Weng J

Sat Oct 01 00:00:00 EDT 2022

Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration.

Molecular therapy : the journal of the American Society of Gene Therapy

Wu J,Chen Y,Liao Z,Liu H,Zhang S,Zhong D,Qiu X,Chen T,Su D,Ke X,Wan Y,Zhou T,Su P

Wed Oct 05 00:00:00 EDT 2022

Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration.

Molecular therapy : the journal of the American Society of Gene Therapy

Wu J,Chen Y,Liao Z,Liu H,Zhang S,Zhong D,Qiu X,Chen T,Su D,Ke X,Wan Y,Zhou T,Su P

Wed Oct 05 00:00:00 EDT 2022

Periostin promotes the proliferation and metastasis of osteosarcoma by increasing cell survival and activates the PI3K/Akt pathway.

Cancer cell international

Xu C,Wang Z,Zhang L,Feng Y,Lv J,Wu Z,Yang R,Wu T,Li J,Zhou R,Tian Z,Bai J,Zhang H,Lan Y,Lv Z

Thu Jan 20 00:00:00 EST 2022

Periostin promotes the proliferation and metastasis of osteosarcoma by increasing cell survival and activates the PI3K/Akt pathway.

Cancer cell international

Xu C,Wang Z,Zhang L,Feng Y,Lv J,Wu Z,Yang R,Wu T,Li J,Zhou R,Tian Z,Bai J,Zhang H,Lan Y,Lv Z

Thu Jan 20 00:00:00 EST 2022

Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.

Biochemical and biophysical research communications

Liu J,He Y,Cheng K,Xie P

Thu Mar 26 00:00:00 EDT 2020

Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide Synthase Expression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats.

International journal of molecular sciences

Imoto K,Okada M,Yamawaki H

Mon Dec 24 00:00:00 EST 2018

International journal of molecular sciences

Imoto K,Okada M,Yamawaki H

Mon Dec 24 00:00:00 EST 2018

Bioinformatics

Protein Aliases: fasciclin I-like; Fasciclin-I like; MGC119510; MGC119511; OSF 2; OSF-2; osteoblast specific factor 2; osteoblast specific factor 2 (fasciclin I-like); Osteoblast-specific factor 2; periodontal ligament-specific periostin; Periostin; periostin, osteoblast specific factor; periostin-like factor; PN; RP11 412K4.1; RP11-412K4.1

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Gene Aliases: A630052E07Rik; AI747096; OSF-2; OSF2; PDLPOSTN; peri; PLF; PN; POSTN

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UniProt ID: (Human) Q15063, (Mouse) Q62009

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Entrez Gene ID: (Human) 10631, (Rat) 361945, (Mouse) 50706

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Function(s)

protein binding heparin binding metal ion binding cell adhesion molecule binding cell adhesion molecule

Process(es)

skeletal system development response to hypoxia negative regulation of cell-matrix adhesion regulation of systemic arterial blood pressure cell adhesion regulation of Notch signaling pathway response to mechanical stimulus response to muscle activity positive regulation of smooth muscle cell migration extracellular matrix organization response to estradiol cellular response to fibroblast growth factor stimulus cellular response to vitamin K cellular response to tumor necrosis factor cellular response to transforming growth factor beta stimulus negative regulation of substrate adhesion-dependent cell spreading positive regulation of chemokine (C-C motif) ligand 2 secretion neuron projection extension bone regeneration tissue development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Periostin Monoclonal Antibody (1A11A3)

Periostin Antibody (66491-1-IG) in IHC (P)

Product Details

66491-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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