Invitrogen

Phospho-A-Raf/B-Raf/C-Raf (Ser446, Ser338, Ser299) Polyclonal Antibody

View all (2) A-Raf/B-Raf/C-Raf antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-143756

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Phospho-B-Raf (Ser-446) synthetic peptide (coupled to KLH) corresponds to amino acids surrounding serine 446 in human B-Raf. This sequence is conserved in B-Rafs from many species, and has high homology to C-Raf (Ser-338) and A-Raf (Ser-299).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942984

Product Specific Information

This antibody was cross-adsorbed to phospho-B-Raf (Ser-579) peptide before affinity purification using phospho-B-Raf (Ser-446) peptide. The purified antibody detects a strong band at 95 kDa* and a weaker band at 105 kDa in western blots of human Jurkat or A431 cells treated with Calyculin A. The antibody also detects other phosphorylated proteins at 150 and 200 kDa in calyculin A treated cells.

Target Information

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF1; C-RAF; C-Raf proto-oncogene, serine/threonine kinase; CRaf; Craf1 transforming; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; murine sarcoma viral (v-raf) oncogene homolog B1; Oncogene RAF1; p94; Proto-oncogene B-Raf; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; Raf1; RAFB1; sarcoma 3611 oncogene; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1

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Gene Aliases: 6430402F14Rik; 9930012E13Rik; AA120551; AA387315; AA473386; AA990557; B-raf; B-RAF1; BB129353; BRAF; Braf-2; BRAF1; Braf2; c-Raf; C230098H17; C87398; CMD1NN; CRAF; Craf1; D6Ertd631e; D830050J10Rik; NS5; NS7; RAF; Raf-1; RAF1; RAFB1; v-Raf

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UniProt ID: (Human) P15056, (Mouse) P28028, (Human) P04049, (Mouse) Q99N57, (Rat) P11345

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Entrez Gene ID: (Human) 673, (Rat) 114486, (Mouse) 109880, (Human) 5894, (Mouse) 110157, (Rat) 24703

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding protein heterodimerization activity nucleotide binding receptor signaling protein activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding Ras GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPKK activity myeloid progenitor cell differentiation protein phosphorylation visual learning organ morphogenesis positive regulation of gene expression negative regulation of fibroblast migration glucose transport thyroid gland development positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance cellular response to drug regulation of cell proliferation negative regulation of apoptotic process CD4-positive, alpha-beta T cell differentiation positive T cell selection response to peptide hormone negative regulation of neuron apoptotic process thymus development positive regulation of axon regeneration positive regulation of axonogenesis protein heterooligomerization positive regulation of stress fiber assembly response to cAMP long-term synaptic potentiation head morphogenesis face development positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion establishment of protein localization to membrane positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process cell differentiation alpha-beta T cell differentiation regulation of axon regeneration signal transduction phosphorylation intracellular signal transduction response to hypoxia stimulatory C-type lectin receptor signaling pathway apoptotic process activation of adenylate cyclase activity heart development cell proliferation negative regulation of cell proliferation platelet activation negative regulation of protein complex assembly ion transmembrane transport regulation of Rho protein signal transduction insulin secretion involved in cellular response to glucose stimulus response to muscle stretch wound healing regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process intermediate filament cytoskeleton organization regulation of cell differentiation positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors regulation of cell motility cellular glucose homeostasis positive regulation of protein phosphorylation
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