Invitrogen

Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

34 Published Figures

50 References

View all (189) AKT1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Phospho-AKT1 (Ser473) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 43

Phospho-AKT1 (Ser473) Antibody (44-621G) in ICC/IF

Immunofluorescent analysis of phospho-AKT pSer473 (green) in HeLa cells either serum starved overnight (left panel) or treated with 10% FBS (right panel) for 30 minutes. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a phospho-AKT pSer473 mono... View More

Phospho-AKT1 (Ser473) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-AKT1 (Ser473) Antibody in Western Blot (WB)

Phospho-AKT1 (Ser473) Antibody in Immunohistochemistry (IHC)

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Product Details

44-621G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 47 publications 47 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

Immunocytochemistry (ICC/IF)

1:50-1:200

in situ PLA (PLA)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Chicken, Dog, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

14-6

Immunogen

The antibody was produced against a chemically synthesized phosphopeptide derived from the region of human Akt that contains serine 473.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA, 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533699

Product Specific Information

This product contains enough material for 10 mini-blots. The immunogenic sequence is conserved among multiple species including human, mouse, and rat in all three isoforms: Akt1, Akt2, and Akt3. Others species (100% homologous) have not been tested. Recommended positive controls include NIH-3T3 cells stimulated with PDGF and 3T3-L1 adipocytes stimulated with OSM and IFN-gamma.

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cholecystokinin Receptor Antagonist Induces Pancreatic Stellate Cell Plasticity Rendering the Tumor Microenvironment Less Oncogenic.

Cancers

Jolly G,Duka T,Shivapurkar N,Chen W,Bansal S,Cheema A,Smith JP

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Thu May 18 00:00:00 EDT 2023

Mechanism of TNFα-induced downregulation of salt-inducible kinase 2 in adipocytes.

Scientific reports

Vaváková M,Hofwimmer K,Laurencikiene J,Göransson O

44-621G was used in Western Blotting to show that TNFα downregulates SIK2 protein expression not only in 3T3L1- but also in human in vitro differentiated adipocytes.

Thu Jun 29 00:00:00 EDT 2023

Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling.

The Journal of cell biology

Cabral-Dias R,Lucarelli S,Zak K,Rahmani S,Judge G,Abousawan J,DiGiovanni LF,Vural D,Anderson KE,Sugiyama MG,Genc G,Hong W,Botelho RJ,Fairn GD,Kim PK,Antonescu CN

44-621G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to examine how CCPs control EGFR signaling.

Mon Apr 04 00:00:00 EDT 2022

Fyn and TOM1L1 are recruited to clathrin-coated pits and regulate Akt signaling.

The Journal of cell biology

Cabral-Dias R,Lucarelli S,Zak K,Rahmani S,Judge G,Abousawan J,DiGiovanni LF,Vural D,Anderson KE,Sugiyama MG,Genc G,Hong W,Botelho RJ,Fairn GD,Kim PK,Antonescu CN

44-621G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to examine how CCPs control EGFR signaling.

Mon Apr 04 00:00:00 EDT 2022

LncRNA FOXD3-AS1 aggravates myocardial ischemia/reperfusion injury by inactivating the Redd1/AKT/GSK3β/Nrf2 signaling pathway via the miR-128/TXNIP axis.

Journal of biochemical and molecular toxicology

Chen B,Zheng L,Zhu T,Jiao K

44-621G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that both inhibition of miR-128 and overexpression of TXNIP reversed the cardioprotective effect of FOXD3-AS1 small interfering RNA in H/R-induced H9c2 cells.

Thu Dec 01 00:00:00 EST 2022

The phosphatase and tensin homolog gene inserted between NP and P gene of recombinant New castle disease virus oncolytic effect test to glioblastoma cell and xenograft mouse model.

Virology journal

Jang SH,Jung BK,An YH,Jang H

44-621G was used in Western Blot to demonstrate that NP and P gene site is better than P and M gene site which is commonly and conventionally used.

Sat Jan 29 00:00:00 EST 2022

Choline and nicotine increase glioblastoma cell proliferation by binding and activating α7- and α9- containing nicotinic receptors.

Pharmacological research

Pucci S,Fasoli F,Moretti M,Benfante R,Di Lascio S,Viani P,Daga A,Gordon TJ,McIntosh M,Zoli M,Clementi F,Gotti C

44-621G was used in Western Blot to study the expression of neuronal nicotinic acetylcholine receptor in glioblastoma cells.

Fri Jan 01 00:00:00 EST 2021

D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes.

Frontiers in endocrinology

Montt-Guevara MM,Finiguerra M,Marzi I,Fidecicchi T,Ferrari A,Genazzani AD,Simoncini T

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Wed Jan 12 00:00:00 EST 2022

The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes.

American journal of physiology. Lung cellular and molecular physiology

Wu Y,Huang Y,Zhang W,Gunst SJ

44-621G was used in Western Blotting to demonstrate that furin inhibits inflammation in airway smooth muscle induced by IL-13 and that the anti-inflammatory effects of furin are mediated by activating integrin proteins and integrin-associated signaling complexes that regulate Akt-mediated pathways to the nucleus.

Thu Jul 01 00:00:00 EDT 2021

IGF1 does not overcome sexual dimorphism of body and muscle size in Mstn-/- mice.

The Journal of endocrinology

Paul R,Whiteman K,Falconer SJ,Oldham JM,Jeanplong F,Matthews KG,Smith HK,Thomas M,Watson T,McMahon CD

44-621G was used in Western Blot to clarify the roles of these Insulin-like growth factor-1 and myostatin in regulating sexually dimorphic growth of body and muscle mass.

Mon Feb 01 00:00:00 EST 2021

Downregulation of miR-375 contributes to ERBB2-mediated VEGFA overexpression in esophageal cancer.

Journal of Cancer

Ren S,Tan X,Fu MZ,Ren S,Wu X,Chen T,Latham PS,Lin P,Man YG,Fu SW

44-621G was used in Western Blot to demonstrate that microRNA-375 (miR-375) is one of the most frequently downregulated miRNAs in esophageal cancer.

Fri Nov 05 00:00:00 EDT 2021

A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner.

The Biochemical journal

Kopietz F,Alshuweishi Y,Bijland S,Alghamdi F,Degerman E,Sakamoto K,Salt IP,Göransson O

44-621G was used in Western Blot to suggest that AMPK activation per se does not inhibit glucose uptake in adipocytes and that the effects of AICAR and A-769662 are AMPK-independent.

Fri Feb 12 00:00:00 EST 2021

Implications of cell division cycle associated 4 on the Wilm's tumor cells viability via AKT/mTOR signaling pathway.

Renal failure

Li S,Qin C,Chen Y,Wei D,Tan Z,Meng J

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Wed Dec 01 00:00:00 EST 2021

MiR-133a-3p attenuates resistance of non-small cell lung cancer cells to gefitinib by targeting SPAG5.

Journal of clinical laboratory analysis

Li Q,Wang Y,He J

44-621G was used in Western Blot to explore the effect and mechanism of miR-133a-3p on the gefitinib sensitivity of non-small cell lung cancer cells.

Thu Jul 01 00:00:00 EDT 2021

MiR-133a-3p attenuates resistance of non-small cell lung cancer cells to gefitinib by targeting SPAG5.

Journal of clinical laboratory analysis

Li Q,Wang Y,He J

44-621G was used in Western Blot to explore the effect and mechanism of miR-133a-3p on the gefitinib sensitivity of non-small cell lung cancer cells.

Thu Jul 01 00:00:00 EDT 2021

DNA methyltransferase 3 beta mediates the methylation of the microRNA-34a promoter and enhances chondrocyte viability in osteoarthritis.

Bioengineered

Xiong S,Zhao Y,Xu T

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Wed Dec 01 00:00:00 EST 2021

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Tue Mar 23 00:00:00 EDT 2021

Targeting Neuregulin 1 (NRG1): A Novel Biomarker for Non-Small-Cell Lung Cancer.

Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer

Fang C,Kang B,Zhao P,Ran J,Wang L,Zhao L,Luo H,Tao L

44-621G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to identify the roles of neuregulin 1 (NRG1) during the tumor progression in non-small-cell lung cancer (NSCLC).

Mon Jan 03 00:00:00 EST 2022

Phenotype transitions induced by mechanical stimuli in airway smooth muscle are regulated by differential interactions of parvin isoforms with paxillin and Akt.

American journal of physiology. Lung cellular and molecular physiology

Huang Y,Gunst SJ

44-621G was used in Proximity Ligation Assay (PLA) to describe a novel molecular mechanism for transduction of mechanical and humoral stimuli within integrin signaling complexes to regulate phenotype expression in airway smooth muscle.

Fri May 01 00:00:00 EDT 2020

Phenotype transitions induced by mechanical stimuli in airway smooth muscle are regulated by differential interactions of parvin isoforms with paxillin and Akt.

American journal of physiology. Lung cellular and molecular physiology

Huang Y,Gunst SJ

Fri May 01 00:00:00 EDT 2020

Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels.

American journal of physiology. Endocrinology and metabolism

Kopietz F,Rupar K,Berggreen C,Säll J,Vertommen D,Degerman E,Rider MH,Göransson O

44-621G was used in Western Blot to improve the understanding of how insulin regulates AMP-activated protein kinase activity, and with that, de novo fatty acid synthesis, in adipose tissue.

Tue Sep 01 00:00:00 EDT 2020

Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model.

International journal of molecular medicine

Inprasit C,Huang YC,Lin YW

44-621G was used in Western Blotting to show body weight maintenance may be modulated by calcium ion channel TRPV1.

Sun Mar 01 00:00:00 EST 2020

Evidence for acupoint catgut embedding treatment and TRPV1 gene deletion increasing weight control in murine model.

International journal of molecular medicine

Inprasit C,Huang YC,Lin YW

44-621G was used in Western Blotting to show body weight maintenance may be modulated by calcium ion channel TRPV1.

Sun Mar 01 00:00:00 EST 2020

Neuroprotective Effects of Growth Hormone (GH) and Insulin-Like Growth Factor Type 1 (IGF-1) after Hypoxic-Ischemic Injury in Chicken Cerebellar Cell Cultures.

International journal of molecular sciences

Baltazar-Lara R,Ávila-Mendoza J,Martínez-Moreno CG,Carranza M,Pech-Pool S,Vázquez-Martínez O,Díaz-Muñoz M,Luna M,Arámburo C

44-621G was used in Western Blot to show that GH, either exogenously administered and/or locally expressed, can act as a neuroprotective factor in response to hypoxic-ischemic injury, and that this effect may be mediated, at least partially, through IGF-1 expression.

Tue Dec 29 00:00:00 EST 2020

Neuroprotective Effects of Growth Hormone (GH) and Insulin-Like Growth Factor Type 1 (IGF-1) after Hypoxic-Ischemic Injury in Chicken Cerebellar Cell Cultures.

International journal of molecular sciences

Baltazar-Lara R,Ávila-Mendoza J,Martínez-Moreno CG,Carranza M,Pech-Pool S,Vázquez-Martínez O,Díaz-Muñoz M,Luna M,Arámburo C

Tue Dec 29 00:00:00 EST 2020

Jejunal Insulin Signalling Is Increased in Morbidly Obese Subjects with High Insulin Resistance and Is Regulated by Insulin and Leptin.

Journal of clinical medicine

Gutierrez-Repiso C,Ho-Plagaro A,Santiago-Fernandez C,Garcia-Serrano S,Rodríguez-Pacheco F,Valdes S,Garrido-Sanchez L,Rodríguez-Díaz C,López-Gómez C,Moreno-Ruiz FJ,Alcain-Martinez G,Gautier-Stein A,Mithieux G,Garcia-Fuentes E

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Fri Jan 10 00:00:00 EST 2020

Jejunal Insulin Signalling Is Increased in Morbidly Obese Subjects with High Insulin Resistance and Is Regulated by Insulin and Leptin.

Journal of clinical medicine

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Fri Jan 10 00:00:00 EST 2020

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

International journal of molecular sciences

Rajendran P,Ammar RB,Al-Saeedi FJ,Mohamed ME,ElNaggar MA,Al-Ramadan SY,Bekhet GM,Soliman AM

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Mon Dec 28 00:00:00 EST 2020

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

International journal of molecular sciences

Rajendran P,Ammar RB,Al-Saeedi FJ,Mohamed ME,ElNaggar MA,Al-Ramadan SY,Bekhet GM,Soliman AM

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Mon Dec 28 00:00:00 EST 2020

Systematic Development and Optimization of Inhalable Pirfenidone Liposomes for Non-Small Cell Lung Cancer Treatment.

Pharmaceutics

Parvathaneni V,Kulkarni NS,Shukla SK,Farrales PT,Kunda NK,Muth A,Gupta V

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Fri Feb 28 00:00:00 EST 2020

Systematic Development and Optimization of Inhalable Pirfenidone Liposomes for Non-Small Cell Lung Cancer Treatment.

Pharmaceutics

Parvathaneni V,Kulkarni NS,Shukla SK,Farrales PT,Kunda NK,Muth A,Gupta V

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Fri Feb 28 00:00:00 EST 2020

Phlpp1 is associated with human intervertebral disc degeneration and its deficiency promotes healing after needle puncture injury in mice.

Cell death & disease

Zhang C,Smith MP,Zhou GK,Lai A,Hoy RC,Mroz V,Torre OM,Laudier DM,Bradley EW,Westendorf JJ,Iatridis JC,Illien-Jünger S

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Immunohistochemistry

Thu Oct 03 00:00:00 EDT 2019

Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R.

International journal of molecular sciences

Chalenko Y,Kalinin E,Marchenkov V,Sysolyatina E,Surin A,Sobyanin K,Ermolaeva S

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Sat Aug 24 00:00:00 EDT 2019

Ascites from ovarian cancer patients stimulates MUC16 mucin expression and secretion in human peritoneal mesothelial cells through an Akt-dependent pathway.

BMC cancer

Matte I,Garde-Granger P,Bessette P,Piché A

44-621G was used in Western Blotting to investigate whether ovarian cancer ascites stimulate the expression and release of MUC16 by human peritoneal mesothelial cells.

Tue Apr 30 00:00:00 EDT 2019

Induction of apoptotic but not autophagic cell death by Cinnamomum cassia extracts on human oral cancer cells.

Journal of cellular physiology

Yu CH,Chu SC,Yang SF,Hsieh YS,Lee CY,Chen PN

44-621G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to exhibit that CCE reduces the viability, number, and colony formation of human oral cancer cells, and induces their apoptosis.

Mon Apr 01 00:00:00 EDT 2019

A rich medium-chain triacylglycerol diet benefits adiposity but has adverse effects on the markers of hepatic lipogenesis and beta-oxidation.

Food & function

Chamma CM,Bargut TC,Mandarim-de-Lacerda CA,Aguila MB

44-621G was used in western blot to describe the effects of dietary medium-chain triacylglycerols on hepatic lipid metabolism

Wed Feb 22 00:00:00 EST 2017

Differential effects of angiotensin receptor blockers on pancreatic islet remodelling and glucose homeostasis in diet-induced obese mice.

Molecular and cellular endocrinology

Graus-Nunes F,Marinho TS,Barbosa-da-Silva S,Aguila MB,Mandarim-de-Lacerda CA,Souza-Mello V

44621G was used in western blot to compare the effects of losartan and telmisartan on pancreatic islets remodeling and glucose homeostasis in diet-induced obese mice

Thu Jan 05 00:00:00 EST 2017

Thermogenesis, fatty acid synthesis with oxidation, and inflammation in the brown adipose tissue of ob/ob (-/-) mice.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Martins FF,Bargut TCL,Aguila MB,Mandarim-de-Lacerda CA

44621G was used in western blot to find that the lack of substrate for thermogenesis and the local inflammation negatively regulated thermogenic signaling in the ob/ob mice

Wed Mar 01 00:00:00 EST 2017

Visualization of lipid directed dynamics of perilipin 1 in human primary adipocytes.

Scientific reports

Hansen JS,de Maré S,Jones HA,Göransson O,Lindkvist-Petersson K

44-621G was used in Western Blotting to suggest a simple unifying mechanism that lipid assembly and segregation control lipolysis in human primary adipocytes.

Wed Nov 08 00:00:00 EST 2017

Adverse effects of vitamin D deficiency on the Pi3k/Akt pathway and pancreatic islet morphology in diet-induced obese mice.

Molecular nutrition & food research

Borges CC,Salles AF,Bringhenti I,Souza-Mello V,Mandarim-de-Lacerda CA,Aguila MB

44-621G was used in western blot to examine the impact of vitamin D deficiency on insulin resistance and abnormal glucose homeostasis in obesity.

Mon Feb 01 00:00:00 EST 2016

Evidence for the involvement of the CXCL12 system in the adaptation of skeletal muscles to physical exercise.

Cellular signalling

Puchert M,Adams V,Linke A,Engele J

44-621G was used in western blot to determine the involvement of the CXCL12 system in adapting skeletal muscles to physical exercise

Thu Sep 01 00:00:00 EDT 2016

DMSO Represses Inflammatory Cytokine Production from Human Blood Cells and Reduces Autoimmune Arthritis.

PloS one

Elisia I,Nakamura H,Lam V,Hofs E,Cederberg R,Cait J,Hughes MR,Lee L,Jia W,Adomat HH,Guns ES,McNagny KM,Samudio I,Krystal G

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Fri Aug 12 00:00:00 EDT 2016

Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms.

International journal of cancer

Matte I,Lane D,Laplante C,Garde-Granger P,Rancourt C,Piché A

44-621G was used in western blot to suggest that HGF and EGFR signaling mediate ovarian cancer ascites-mediated migration of human peritoneal mesothelial cells

Wed Jul 15 00:00:00 EDT 2015

A high-fish-oil diet prevents adiposity and modulates white adipose tissue inflammation pathways in mice.

The Journal of nutritional biochemistry

Bargut TC,Mandarim-de-Lacerda CA,Aguila MB

44-621G was used in western blot to study the regulatory effect of a high-fish-oil diet on rodent white adipose tissue inflammation pathways

Tue Sep 01 00:00:00 EDT 2015

p53 attenuates AKT signaling by modulating membrane phospholipid composition.

Oncotarget

Rueda-Rincon N,Bloch K,Derua R,Vyas R,Harms A,Hankemeier T,Khan NA,Dehairs J,Bagadi M,Binda MM,Waelkens E,Marine JC,Swinnen JV

44-621G was used in western blot to report a role for p53 in regulating the membrane phospholipids composition.

Fri Aug 28 00:00:00 EDT 2015

Hematopoiesis and RAS-driven myeloid leukemia differentially require PI3K isoform p110α.

The Journal of clinical investigation

Gritsman K,Yuzugullu H,Von T,Yan H,Clayton L,Fritsch C,Maira SM,Hollingworth G,Choi C,Khandan T,Paktinat M,Okabe RO,Roberts TM,Zhao JJ

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Tue Apr 01 00:00:00 EDT 2014

Ganitumab (AMG 479) inhibits IGF-II-dependent ovarian cancer growth and potentiates platinum-based chemotherapy.

Clinical cancer research : an official journal of the American Association for Cancer Research

Beltran PJ,Calzone FJ,Mitchell P,Chung YA,Cajulis E,Moody G,Belmontes B,Li CM,Vonderfecht S,Velculescu VE,Yang G,Qi J,Slamon DJ,Konecny GE

44-621G was used in western blot to assess the therapeutic potential of ganitumab for the treatment of ovarian cancer.

Sun Jun 01 00:00:00 EDT 2014

Noncovalent wild-type-sparing inhibitors of EGFR T790M.

Cancer discovery

Lee HJ,Schaefer G,Heffron TP,Shao L,Ye X,Sideris S,Malek S,Chan E,Merchant M,La H,Ubhayakar S,Yauch RL,Pirazzoli V,Politi K,Settleman J

44-621G was used in western blot to identify indolocarbazole compounds as potentially effective EGFR inhibitors.

Fri Feb 01 00:00:00 EST 2013

PLC-γ and PI3K link cytokines to ERK activation in hematopoietic cells with normal and oncogenic Kras.

Science signaling

Diaz-Flores E,Goldschmidt H,Depeille P,Ng V,Akutagawa J,Krisman K,Crone M,Burgess MR,Williams O,Houseman B,Shokat K,Sampath D,Bollag G,Roose JP,Braun BS,Shannon K

44-621G was used in western blot to demonstrate that phospholipase C-γ, PI3K, and their generated second messengers link activated cytokine receptors to Ras and ERK signaling in differentiated bone marrow cells and leukemia stem cells.

Tue Dec 03 00:00:00 EST 2013

Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.

Nature

Wilson TR,Fridlyand J,Yan Y,Penuel E,Burton L,Chan E,Peng J,Lin E,Wang Y,Sosman J,Ribas A,Li J,Moffat J,Sutherlin DP,Koeppen H,Merchant M,Neve R,Settleman J

44-621G was used in western blot to study the effect of receptor tyrosine kinases ligands on cancer resistance.

Thu Jul 26 00:00:00 EDT 2012

The role of SHIP in the development and activation of mouse mucosal and connective tissue mast cells.

Journal of immunology (Baltimore, Md. : 1950)

Ruschmann J,Antignano F,Lam V,Snyder K,Kim C,Essak M,Zhang A,Lin AH,Mali RS,Kapur R,Krystal G

Sun Apr 15 00:00:00 EDT 2012

The role of SHIP in the development and activation of mouse mucosal and connective tissue mast cells.

Journal of immunology (Baltimore, Md. : 1950)

Ruschmann J,Antignano F,Lam V,Snyder K,Kim C,Essak M,Zhang A,Lin AH,Mali RS,Kapur R,Krystal G

Sun Apr 15 00:00:00 EDT 2012

Type I IFN receptor and the B cell antigen receptor regulate TLR7 responses via distinct molecular mechanisms.

Journal of immunology (Baltimore, Md. : 1950)

Poovassery JS,Bishop GA

44-621G was used in western blot to test if BCR-mediated effects on TLR7 tolerance are mediated by type I IFN receptor signals.

Wed Aug 15 00:00:00 EDT 2012

Protein phosphatase magnesium dependent 1A (PPM1A) plays a role in the differentiation and survival processes of nerve cells.

PloS one

Shohat M,Ben-Meir D,Lavi S

44-621G was used in western blot to study the role of PPMA in the survival and differentiation processes of PC6-3 cells.

Mon Aug 27 00:00:00 EDT 2012

Neuregulin-1-mediated autocrine signaling underlies sensitivity to HER2 kinase inhibitors in a subset of human cancers.

Cancer cell

Wilson TR,Lee DY,Berry L,Shames DS,Settleman J

44-621G was used in western blot to suggest that lapatinib may benefit patients with NRG-driven tumors lacking HER2 amplification.

Tue Aug 16 00:00:00 EDT 2011

The absence of caveolin-1 increases proliferation and anchorage- independent growth by a Rac-dependent, Erk-independent mechanism.

Molecular and cellular biology

Cerezo A,Guadamillas MC,Goetz JG,Sánchez-Perales S,Klein E,Assoian RK,del Pozo MA

44-621G was used in western blot to elucidate the mechanisms by which loss of Cav1 function promotes cancer cell growth

Tue Sep 01 00:00:00 EDT 2009

Deregulated NFATc1 activity transforms murine fibroblasts via an autocrine growth factor-mediated Stat3-dependent pathway.

Journal of cellular biochemistry

Lagunas L,Clipstone NA

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Tue Sep 01 00:00:00 EDT 2009

Activation of Notch-mediated protective signaling in the myocardium.

Circulation research

Gude NA,Emmanuel G,Wu W,Cottage CT,Fischer K,Quijada P,Muraski JA,Alvarez R,Rubio M,Schaefer E,Sussman MA

Published figure using Phospho-AKT1 (Ser473) monoclonal antibody (Product # 44-621G) in Western Blot

Fri May 09 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; C-AKT; CAKT; PKB; PKB alpha; PKBG; Protein kinase B; Protein kinase B alpha; protein kinase B-alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; related to A and C kinases; serine-threonine protein kinase; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

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Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PKB/Akt; PKBalpha; PRKBA; RAC; RAC-ALPHA

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UniProt ID: (Human) P31749, (Mouse) P31750

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Entrez Gene ID: (Human) 207, (Mouse) 11651

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Function(s)

protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase

Process(es)

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Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6)

Phospho-AKT1 (Ser473) Antibody (44-621G) in ICC/IF

Product Details

44-621G

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

in situ PLA (PLA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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