Invitrogen

Phospho-AMPK alpha-1,2 (Thr183, Thr172) Recombinant Rabbit Monoclonal Antibody (10H2L20)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (16) AMPK alpha-1,2 antibodies
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Datasheet
Protocols
Questions & Answers

Product Details

701068

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

10H2L20

Immunogen

Phosphopeptide corresponding to amino acids 168-178 of human AMPK alpha
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532369

Product Specific Information

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; AAPK2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK alpha 2; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK-alpha-1 chain; AMPK-alpha-2 chain; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; pAMPK; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; Tau-protein kinase PRKAA1

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Gene Aliases: AMPK; AMPK1; AMPK2; AMPKa1; AMPKa2; PRKAA; PRKAA1; PRKAA2

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UniProt ID: (Human) Q13131, (Human) P54646

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Entrez Gene ID: (Human) 5562, (Human) 5563

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Function(s)
chromatin binding protein kinase activity AMP-activated protein kinase activity cAMP-dependent protein kinase activity protein binding ATP binding protein C-terminus binding kinase binding histone serine kinase activity metal ion binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity tau-protein kinase activity [acetyl-CoA carboxylase] kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity non-receptor serine/threonine protein kinase
Process(es)
activation of MAPK activity response to hypoxia glucose metabolic process transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation fatty acid biosynthetic process cholesterol biosynthetic process cell cycle arrest signal transduction positive regulation of cell proliferation lipid biosynthetic process response to UV cold acclimation response to gamma radiation positive regulation of autophagy positive regulation of gene expression response to activity Wnt signaling pathway macroautophagy fatty acid oxidation response to caffeine cellular response to nutrient levels negative regulation of TOR signaling regulation of peptidyl-serine phosphorylation histone-serine phosphorylation intracellular signal transduction cellular response to glucose starvation glucose homeostasis regulation of circadian rhythm negative regulation of apoptotic process positive regulation of cholesterol biosynthetic process positive regulation of glycolytic process negative regulation of glucosylceramide biosynthetic process rhythmic process positive regulation of skeletal muscle tissue development negative regulation of lipid catabolic process protein heterooligomerization fatty acid homeostasis regulation of vesicle-mediated transport cellular response to hydrogen peroxide cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to organonitrogen compound cellular response to hypoxia response to camptothecin regulation of signal transduction by p53 class mediator regulation of energy homeostasis negative regulation of glucose import in response to insulin stimulus carnitine shuttle response to stress response to muscle activity positive regulation of macroautophagy regulation of macroautophagy cellular response to drug regulation of fatty acid biosynthetic process
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