Invitrogen

Phospho-ATF2 (Thr71) Recombinant Rabbit Monoclonal Antibody (SC05-90)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-32204

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

SC05-90

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Thr71 of Human ATF2 aa 58-94
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809491

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB-2; Cyclic AMP-dependent transcription factor ATF-2; Cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; MGC111558; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354

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Gene Aliases: ATF2; CRE-BP1; CREB-2; CREB2; CREBP1; HB16; TREB7

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UniProt ID: (Human) P15336

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Entrez Gene ID: (Human) 1386

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity histone acetyltransferase activity protein binding cAMP response element binding protein binding protein kinase binding cAMP response element binding metal ion binding protein heterodimerization activity basic leucine zipper transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter outflow tract morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter response to osmotic stress cellular response to DNA damage stimulus response to water deprivation histone acetylation intra-S DNA damage checkpoint positive regulation of transforming growth factor beta2 production positive regulation of neuron apoptotic process fat cell differentiation positive regulation of transcription from RNA polymerase II promoter negative regulation of epithelial cell proliferation regulation of sequence-specific DNA binding transcription factor activity positive regulation of sequence-specific DNA binding transcription factor activity adipose tissue development amelogenesis positive regulation of mitochondrial membrane permeability involved in apoptotic process
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