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Phospho-ATG13 (Thr24) Antibody (PA5-105418) in WB
Western blot analysis of Phospho-ATG13 (Thr24) in MCF-7cell lysate (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-ATG13 (Thr24) polyclonal antibody (Product # PA5-105418).
Product Details
PA5-105418
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATG13(Accession O75143), corresponding to amino acid residues around phosphorylated Thr24.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816846
Product Specific Information
Antibody detects endogenous levels of Atg13 only when phosphorylated at Thr24.
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATG13 autophagy related 13 homolog; Autophagy-related protein 13; FLJ20698; harbinger transposase derived 1; OTTHUMP00000233321; OTTHUMP00000233322; OTTHUMP00000233323; OTTHUMP00000233324; OTTHUMP00000233325; OTTHUMP00000233326
Gene Aliases: 1110053A20Rik; ATG13; D2Ertd391e; Harbi1; Harbi1l; KIAA0652; PARATARG8; RGD1310685
UniProt ID: (Human) O75143, (Mouse) Q91YI1
Entrez Gene ID: (Human) 9776, (Mouse) 51897, (Rat) 362164
Performance Guarantee
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