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Invitrogen
Phospho-ATM (Ser1981) Monoclonal Antibody (10H11)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
Phospho-ATM (Ser1981) Antibody (MA1-2020) in ICC/IF
Immunofluorescence analysis of Phospho-ATM pSer1981 was performed using 70% confluent log phase HeLa cells irradiated with UV for 4 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-ATM (Ser 1981) (10H11) Mouse Monoclonal Antibody (Product # MA1... View More
Product Details
MA1-2020
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10H11
Immunogen
Synthetic peptide corresponding to residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1086244
Product Specific Information
MA1-2020 detects the phospho-ATM kinase in human and mouse samples.
MA1-2020 has been successfully used in immunofluorescence, immunoprecipitation and Western blot procedures. By Western blot this antibody detects a ~370 kDa protein representing the phospho-ATM kinase in crude lysates from gamma irradiated HeLa cells. In immunofluorescence procedures, MA1-2020 recognizes the phospho-ATM kinase in irradiated human and mouse fibroblasts.
The MA1-2020 immunogen is a phosphorylated synthetic peptide corresponding to the residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein.
Target Information
Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A-T mutated; A-T mutated homolog; AT mutated; AT mutated protein; Ataxia t; ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated homolog; DKFZp781A0353; EC 2.7.1.37; MGC74674; Serine-protein kinase ATM; TEL1, telomere maintenance 1, homolog
Gene Aliases: AI256621; AT1; ATA; ATC; ATD; ATDC; ATE; ATM; C030026E19Rik; TEL1; TELO1
UniProt ID: (Human) Q13315, (Mouse) E9QNY7
Entrez Gene ID: (Human) 472, (Mouse) 11920
DNA binding
protein serine/threonine kinase activity
DNA-dependent protein kinase activity
protein binding
ATP binding
1-phosphatidylinositol-3-kinase activity
protein complex binding
protein dimerization activity
protein N-terminus binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
phosphotransferase activity, alcohol group as acceptor
histone serine kinase activity
non-receptor serine/threonine protein kinase
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA synthesis involved in DNA repair
strand displacement
response to hypoxia
somitogenesis
pre-B cell allelic exclusion
DNA replication
DNA repair
double-strand break repair via nonhomologous end joining
protein phosphorylation
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
telomere maintenance via telomerase
cell cycle arrest
mitotic spindle assembly checkpoint
reciprocal meiotic recombination
signal transduction
brain development
heart development
determination of adult lifespan
intrinsic apoptotic signaling pathway in response to DNA damage
response to ionizing radiation
regulation of autophagy
histone phosphorylation
peptidyl-serine phosphorylation
negative regulation of B cell proliferation
positive regulation of telomere maintenance via telomerase
positive regulation of histone phosphorylation
V(D)J recombination
phosphatidylinositol-3-phosphate biosynthetic process
peptidyl-serine autophosphorylation
lipoprotein catabolic process
regulation of apoptotic process
positive regulation of apoptotic process
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of neuron apoptotic process
meiotic telomere clustering
protein autophosphorylation
oocyte development
neuron apoptotic process
regulation of telomerase activity
histone mRNA catabolic process
cellular response to gamma radiation
cellular response to X-ray
cellular response to nitrosative stress
signal transduction involved in mitotic G2 DNA damage checkpoint
replicative senescence
establishment of RNA localization to telomere
establishment of macromolecular complex localization to telomere
regulation of cellular response to heat
regulation of signal transduction by p53 class mediator
negative regulation of TORC1 signaling
negative regulation of telomere capping
positive regulation of telomere maintenance via telomere lengthening
positive regulation of telomerase catalytic core complex assembly
DNA damage checkpoint
telomere maintenance
cell cycle
female gamete generation
phosphorylation
histone-serine phosphorylation
phosphatidylinositol phosphorylation
regulation of cell cycle
Performance Guarantee
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