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Invitrogen
Phospho-CDK6 (Thr177) Recombinant Superclonal Antibody (16HCLC)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Phospho-CDK6 (Thr177) Antibody (711588) in ICC/IF
For immunofluorescence analysis, T98G cells were fixed and permeabilized for detection of endogenous CDK6 (pT177) using Anti- CDK6 (pT177) Recombinant Rabbit Polyclonal Antibody (Product # 711588, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using SlowFade® Gold Antifade Mountant... View More
Product Details
711588
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
16HCLC
Immunogen
Peptide corresponding to Human CDK4 (169-175)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2632988
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Horse and Bovine
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Target Information
CDK6 functions as a cell-cycle initiator protein. This protein is coexpressed and copurified with CyclinD1. They are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. CDK6 is essential for cell proliferation within the dentate gyrus of the hippocampus and the cuventricular zone of the lateral ventricles. Mutations in the gene results in microcephaly 12, primary, autosomal recessive (MCPH12).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cell division protein kinase 6; CR2 protein kinase; CRK2; Cyclin-dependent kinase 6; MGC59692; Serine/threonine-protein kinase PLSTIRE
Gene Aliases: 5830411I20; AI504062; CDK6; CDKN6; Crk2; MCPH12; PLSTIRE
UniProt ID: (Human) Q00534, (Mouse) Q64261
Entrez Gene ID: (Human) 1021, (Mouse) 12571
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
cyclin binding
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
G1/S transition of mitotic cell cycle
positive regulation of cell-matrix adhesion
type B pancreatic cell development
protein phosphorylation
cell cycle arrest
Notch signaling pathway
negative regulation of cell proliferation
response to virus
regulation of gene expression
positive regulation of gene expression
astrocyte development
dentate gyrus development
lateral ventricle development
T cell differentiation in thymus
gliogenesis
cell dedifferentiation
negative regulation of cell differentiation
negative regulation of myeloid cell differentiation
regulation of erythrocyte differentiation
negative regulation of monocyte differentiation
negative regulation of osteoblast differentiation
negative regulation of cell cycle
positive regulation of fibroblast proliferation
generation of neurons
negative regulation of epithelial cell proliferation
cell division
hematopoietic stem cell differentiation
cellular response to phorbol 13-acetate 12-myristate
regulation of cell motility
negative regulation of cellular senescence
hematopoietic progenitor cell differentiation
cell cycle
phosphorylation
hemopoiesis
cell differentiation
regulation of cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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