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Phospho-CENPA (Ser7) Antibody (PA5-17195) in ICC/IF
Immunofluorescent analysis of Phospho-CENP-A pSer7 in a mitotic HeLa cell using a Phospho-CENP-A pSer7 polyclonal antibody (Product # PA5-17195) (green) and a beta-Tubulin monoclonal antibody (red). Phospho-CENP-A signal is localized to bright spots in the metaphase plate. DNA is labeled using a fluorescent blue dye.
Product Details
PA5-17195
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding pSer7 of human CENP-A protein
Conjugate
Form
Liquid
Concentration
26 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10987142
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other histone proteins, including Histone H3.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A
Gene Aliases: CenH3; CENP-A; CENPA
UniProt ID: (Human) P49450
Entrez Gene ID: (Human) 1058
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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