Invitrogen

Phospho-CREB/ATF1 (Ser133, Ser63) Monoclonal Antibody (10E9)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Published Figure
1 Reference
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-114

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500
-

Immunocytochemistry (ICC/IF)

1:20-1:200
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Flow Cytometry (Flow)

1 µg/test
-

ELISA (ELISA)

0.05 µg/mL
-

ChIP assay (ChIP)

5 µL/10^6 cells
-
Product Specifications

Species Reactivity

Dog, Human, Mouse

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10E9

Immunogen

A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1 mg/mL

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.2, with sucrose, PEG, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2536825

Product Specific Information

This antibody recognizes CREB protein phosphorylated at serine 133 and ATF1 phosphorylated at serine 63. The antibody was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Reconstitute with 1 mL H2O (15 min, RT)

Aliquot and freeze in liquid nitrogen. Antibody can be stored frozen at -80ºC for up to 1 year. Thaw aliquots at 37ºC; thawed aliquots may be stored at 4ºC for up to 3 months. Avoid repeated freeze/thaw cycles.

This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Target Information

Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Activating transcription factor 1; active transcription factor CREB; cAMP-dependent transcription factor ATF-1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-dependent transcription factor ATF-1; Cyclic AMP-responsive element-binding protein 1; Protein TREB36; Ser133; TCR-ATF1; transactivator protein

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Gene Aliases: 2310001E10Rik; 3526402H21Rik; ATF1; AV083133; CREB; CREB-1; CREB1; EWS-ATF1; FUS/ATF-1; TREB36

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UniProt ID: (Human) P18846, (Mouse) P81269, (Human) P16220, (Mouse) Q01147

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Entrez Gene ID: (Dog) 486545, (Human) 466, (Mouse) 11908, (Dog) 607922, (Human) 1385, (Mouse) 12912

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding protein complex binding protein heterodimerization activity DNA binding sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription cofactor activity enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding double-stranded DNA binding transcription factor binding transcription regulatory region DNA binding
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation transforming growth factor beta receptor signaling pathway axonogenesis lactation memory circadian rhythm regulation of cell size response to organic substance negative regulation of transcription by competitive promoter binding pituitary gland development cell differentiation mammary gland development positive regulation of transforming growth factor beta3 production secretory granule organization response to glucagon positive regulation of multicellular organism growth response to drug regulation of circadian rhythm regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion positive regulation of lipid biosynthetic process rhythmic process protein stabilization lung epithelium development lung saccule development Type I pneumocyte differentiation cellular response to zinc ion cellular response to growth factor stimulus regulation of transcription from RNA polymerase II promoter positive regulation of neuron projection development cellular protein complex assembly positive regulation of DNA replication response to organic cyclic compound response to cobalt ion response to hypoxia signal transduction axon guidance aging visual learning response to activity viral process chemotaxis to arachidonic acid response to nicotine cellular response to hepatocyte growth factor stimulus cellular response to platelet-derived growth factor stimulus response to antidepressant positive regulation of apoptotic process regulation of fibroblast proliferation regulation of glial cell proliferation cellular response to fatty acid positive regulation of long-term synaptic potentiation negative regulation of neuron death response to L-glutamate cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus
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