Rockland

Phospho-CREB (Ser133) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-270

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL
-

ELISA (ELISA)

1:1,000-1:6,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CREB phospho peptide corresponding to amino acid residues 122-147 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.064 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: active transcription factor CREB; cAMP response element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein

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Gene Aliases: 2310001E10Rik; 3526402H21Rik; AV083133; CREB; CREB-1; CREB1

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UniProt ID: (Human) P16220, (Mouse) Q01147, (Rat) P15337

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Entrez Gene ID: (Human) 1385, (Mouse) 12912, (Rat) 81646

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor activity protein binding enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding DNA binding double-stranded DNA binding transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation transforming growth factor beta receptor signaling pathway axonogenesis lactation memory circadian rhythm regulation of cell size response to organic substance negative regulation of transcription by competitive promoter binding pituitary gland development cell differentiation mammary gland development positive regulation of transforming growth factor beta3 production secretory granule organization response to glucagon positive regulation of multicellular organism growth response to drug regulation of circadian rhythm regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion positive regulation of lipid biosynthetic process rhythmic process protein stabilization lung epithelium development lung saccule development Type I pneumocyte differentiation cellular response to zinc ion cellular response to growth factor stimulus response to hypoxia signal transduction axon guidance aging visual learning response to activity viral process chemotaxis to arachidonic acid response to nicotine cellular response to hepatocyte growth factor stimulus cellular response to platelet-derived growth factor stimulus response to antidepressant positive regulation of apoptotic process regulation of fibroblast proliferation regulation of glial cell proliferation cellular response to fatty acid positive regulation of long-term synaptic potentiation negative regulation of neuron death response to L-glutamate cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus
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