Invitrogen

Phospho-CREB (Ser133) Monoclonal Antibody (F.959.4)

5 Published Figures
8 References
View all (63) CREB antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-11192

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 5 publications 5 publications

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:1,600
-

Immunohistochemistry (Frozen) (IHC (F))

1:400-1:1,600
-

Immunocytochemistry (ICC/IF)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

1:400-1:1,600
-

ChIP assay (ChIP)

1:50
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

F.959.4

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer133 of human CREB

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

58 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10986840

Product Specific Information

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.

It is not recommended to aliquot this antibody.

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer’s Disease, histiocytoma and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: active transcription factor CREB; cAMP response element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein

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Gene Aliases: 2310001E10Rik; 3526402H21Rik; AV083133; CREB; CREB-1; CREB1

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UniProt ID: (Human) P16220, (Mouse) Q01147, (Rat) P15337

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Entrez Gene ID: (Human) 1385, (Mouse) 12912, (Rat) 81646

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding transcriptional activator activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription cofactor activity protein binding enzyme binding Hsp70 protein binding histone acetyltransferase binding cAMP response element binding identical protein binding arrestin family protein binding DNA binding double-stranded DNA binding transcription factor binding sequence-specific DNA binding transcription regulatory region DNA binding
Process(es)
transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein phosphorylation transforming growth factor beta receptor signaling pathway axonogenesis lactation memory circadian rhythm regulation of cell size response to organic substance negative regulation of transcription by competitive promoter binding pituitary gland development cell differentiation mammary gland development positive regulation of transforming growth factor beta3 production secretory granule organization response to glucagon positive regulation of multicellular organism growth response to drug regulation of circadian rhythm regulation of apoptotic process positive regulation of fat cell differentiation positive regulation of osteoclast differentiation positive regulation of transcription, DNA-templated positive regulation of RNA polymerase II transcriptional preinitiation complex assembly positive regulation of transcription from RNA polymerase II promoter positive regulation of hormone secretion positive regulation of lipid biosynthetic process rhythmic process protein stabilization lung epithelium development lung saccule development Type I pneumocyte differentiation cellular response to zinc ion cellular response to growth factor stimulus response to hypoxia signal transduction axon guidance aging visual learning response to activity viral process chemotaxis to arachidonic acid response to nicotine cellular response to hepatocyte growth factor stimulus cellular response to platelet-derived growth factor stimulus response to antidepressant positive regulation of apoptotic process regulation of fibroblast proliferation regulation of glial cell proliferation cellular response to fatty acid positive regulation of long-term synaptic potentiation negative regulation of neuron death response to L-glutamate cellular response to nerve growth factor stimulus cellular response to insulin-like growth factor stimulus
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