Invitrogen

Phospho-Caspase 8 (Ser387) Recombinant Superclonal Antibody (10HCLC)

1 Published Figure
2 References
View all (39) Caspase 8 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

710535

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Superclonal

Type

Antibody

Clone

10HCLC

Immunogen

Phosphorylation of the Caspase 8 at the Ser-387 residue
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532734

Product Specific Information

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP-8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase-8; Caspase-8 precursor; Caspase8; EC 3.4.22.61; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; ICE8; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581

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Gene Aliases: ALPS2B; CAP4; Casp-8; CASP8; FLICE; MACH; MCH5

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UniProt ID: (Human) Q14790

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Entrez Gene ID: (Human) 841

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Function(s)
cysteine-type endopeptidase activity death receptor binding tumor necrosis factor receptor binding protein binding peptidase activity cysteine-type peptidase activity ubiquitin protein ligase binding protein complex binding death effector domain binding identical protein binding scaffold protein binding cysteine-type endopeptidase activity involved in apoptotic process cysteine-type endopeptidase activity involved in apoptotic signaling pathway protease
Process(es)
proteolysis apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process cellular component disassembly involved in execution phase of apoptosis cell surface receptor signaling pathway response to cold regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death natural killer cell activation macrophage differentiation response to cobalt ion response to estradiol response to lipopolysaccharide response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway TRAIL-activated apoptotic signaling pathway suppression by virus of host cysteine-type endopeptidase activity involved in apoptotic process T cell activation B cell activation positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling response to ethanol positive regulation of macrophage differentiation positive regulation of proteolysis response to antibiotic protein heterooligomerization proteolysis involved in cellular protein catabolic process syncytiotrophoblast cell differentiation involved in labyrinthine layer development nucleotide-binding oligomerization domain containing signaling pathway cellular response to mechanical stimulus cellular response to organic cyclic compound death-inducing signaling complex assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway execution phase of apoptosis activation of cysteine-type endopeptidase activity activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
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