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Invitrogen
Phospho-Cdc6 (Ser54) Recombinant Rabbit Monoclonal Antibody (SD08-47)
FIGURE: 1 / 6
Phospho-Cdc6 (Ser54) Antibody (MA5-32416) in ICC/IF
Immunocytochemical analysis of Phospho-Cdc6 (Ser54) in HepG2 cells using a Phospho-Cdc6 (Ser54) Monoclonal antibody (Product # MA5-32416) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32416
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD08-47
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser54 of Human Cdc6 aa 41-90
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809694
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cdc18-related protein; CDC6 cell division cycle 6 homolog; CDC6-related protein; Cell division control protein 6 homolog; cell division cycle 6 homolog; HsCdc18; HsCDC6; p62(cdc6)
Gene Aliases: CDC18L; CDC6; HsCDC18; HsCDC6
UniProt ID: (Human) Q99741
Entrez Gene ID: (Human) 990
DNA replication checkpoint
regulation of cyclin-dependent protein serine/threonine kinase activity
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
DNA replication
DNA replication initiation
mitotic nuclear division
traversing start control point of mitotic cell cycle
negative regulation of DNA replication
negative regulation of cell proliferation
regulation of mitotic metaphase/anaphase transition
positive regulation of cytokinesis
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of fibroblast proliferation
cell division
positive regulation of chromosome segregation
cellular response to vasopressin
cellular response to angiotensin
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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