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Phospho-Cenexin-1 (Ser796) Antibody (600-401-A47) in WB
Western Blot of Rabbit Anti-Cenexin-1 pS796. Lane 1: MW. Lane 2: Human Semen Lysate. Lane 3: MCF7 WCL. Lane 4: Molt 4 WCL. Lane 5: HeLa WCL. Load: 10µg per lane. Primary Antibody: 1µg/mL blocked with MB-073 overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in MB-073 for 30 minutes at RT. Expect: 93kDa.
Product Details
600-401-A47
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chimpanzee,
Human,
Rhesus monkey
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for human Cenexin-1 protein phosphorylated at S796. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with Cenexin-1from other sources has not been determined.
Target Information
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cancer/testis antigen 134; Cenexin; cenexin 1; Outer dense fiber of sperm tails protein 2; outer dense fiber of sperm tails, 84-kD; Outer dense fiber protein 2; RP11-187B3.1; sperm tail structural protein; testis tissue sperm-binding protein Li 51e
Gene Aliases: CT134; ODF2; ODF2/1; ODF2/2; ODF84
UniProt ID: (Human) Q5BJF6
Entrez Gene ID: (Human) 4957, (Rhesus monkey) 717505
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