Invitrogen

Phospho-Connexin 43 (Ser373) Polyclonal Antibody

1 Published Figure
5 References
View all (41) Connexin 43 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-64670

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human Connexin 43 (Phospho-Ser373)
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2662693

Product Specific Information

Phospho-Connexin 43 (Ser373) Polyclonal Antibody detects endogenous levels of Connexin 43 only when phosphorylated at Ser373.

Target Information

Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; ODD; ODOD; SDTY3; Syndactyly type III

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Gene Aliases: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA

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UniProt ID: (Human) P17302, (Mouse) P23242, (Rat) P08050

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Entrez Gene ID: (Human) 2697, (Mouse) 14609, (Rat) 24392

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Function(s)
signal transducer activity receptor binding gap junction channel activity protein binding ion transmembrane transporter activity SH3 domain binding PDZ domain binding beta-tubulin binding connexin binding gap junction channel activity involved in cardiac conduction electrical coupling scaffold protein binding gap junction channel activity involved in cell communication by electrical coupling protein domain specific binding transmembrane transporter activity gap junction
Process(es)
osteoblast differentiation in utero embryonic development neuron migration negative regulation of endothelial cell proliferation heart looping regulation of heart rate epithelial cell maturation lens development in camera-type eye chronic inflammatory response positive regulation of glomerular filtration endothelium development atrial ventricular junction remodeling transport apoptotic process muscle contraction signal transduction positive regulation of cytosolic calcium ion concentration cell-cell signaling heart development adult heart development response to pH response to glucose vascular transport positive regulation of gene expression negative regulation of gene expression cell communication by chemical coupling cell communication by electrical coupling positive regulation of cell communication by chemical coupling ATP transport gap junction assembly negative regulation of cell growth regulation of bone mineralization positive regulation of insulin secretion ion transmembrane transport response to fluid shear stress embryonic digit morphogenesis positive regulation of I-kappaB kinase/NF-kappaB signaling skeletal muscle tissue regeneration response to peptide hormone positive regulation of osteoblast differentiation positive regulation of protein catabolic process positive regulation of striated muscle tissue development positive regulation of vasoconstriction positive regulation of vasodilation regulation of bone remodeling blood vessel morphogenesis neuron projection morphogenesis protein oligomerization regulation of calcium ion transport negative regulation of cardiac muscle cell proliferation milk ejection regulation of ventricular cardiac muscle cell membrane repolarization regulation of atrial cardiac muscle cell membrane depolarization regulation of ventricular cardiac muscle cell membrane depolarization negative regulation of wound healing cellular response to mechanical stimulus atrial cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction negative regulation of DNA biosynthetic process regulation of bicellular tight junction assembly positive regulation of behavioral fear response epicardial cell to mesenchymal cell transition cell communication regulation of heart contraction negative regulation of cell proliferation embryonic heart tube development cell-cell junction organization transmembrane transport regulation of blood vessel remodeling cardiac conduction establishment of epithelial cell polarity
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