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Invitrogen
Phospho-Cyclin E (Thr77) Recombinant Rabbit Monoclonal Antibody (SD2025)
FIGURE: 1 / 5
Phospho-Cyclin E (Thr77) Antibody (MA5-32358) in ICC/IF
Immunocytochemical analysis of Cyclin E in HepG2 cells using a Phospho-Cyclin E (Thr77) Monoclonal antibody (Product # MA5-32358) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product Details
MA5-32358
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD2025
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr77 of Human Cyclin E1 aa 51-100
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2809639
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Cyclin E belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cyclin Es; cyclin Et; E-S; G1/S-specific cyclin-E1
Gene Aliases: CCNE; CCNE1; pCCNE1
UniProt ID: (Human) P24864
Entrez Gene ID: (Human) 898
G1/S transition of mitotic cell cycle
regulation of transcription involved in G1/S transition of mitotic cell cycle
antral ovarian follicle growth
liver development
DNA replication initiation
protein phosphorylation
response to purine-containing compound
Wnt signaling pathway
androgen receptor signaling pathway
organ regeneration
cellular response to nutrient
response to estradiol
response to progesterone
response to vitamin E
response to cytokine
response to drug
response to ethanol
positive regulation of cell differentiation
regulation of protein kinase activity
positive regulation of transcription, DNA-templated
cell division
response to corticosterone
response to methylmercury
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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