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Invitrogen
Phospho-Cytokeratin 8 (Ser432) Recombinant Rabbit Monoclonal Antibody (21H19L3)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
Phospho-Cytokeratin 8 (Ser432) Antibody (702176) in ICC/IF
For immunofluorescence analysis CACO-2 cells were fixed and permeabilized for detection of endogenous Phospho-Cytokeratin 8 (Ser432) using Anti-Phospho-Cytokeratin 8 (Ser432) Recombinant Rabbit Monoclonal Antibody (Product # 702176, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained us... View More
Product Details
702176
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
21H19L3
Immunogen
Peptide corresponding to human Phospho-Cytokeratin 8 (Ser 432) (aa429-435)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2662391
Product Specific Information
This antibody is predicted to react with Monkey, Horse, Mouse
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-8; Cytokeratin-8; K8; keratin 8, type II; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8
Gene Aliases: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8
UniProt ID: (Human) P05787
Entrez Gene ID: (Human) 3856
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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