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Phospho-DR6 (Thr460) Antibody (PA5-105426) in ICC/IF
Immunofluorescent analysis of Phospho-DR6 (Thr460) in HepG2 cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-DR6 (Thr460) polyclonal antibody (Product # PA5-105426) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (... View More
Product Details
PA5-105426
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TNFRSF21(Accession O75509), corresponding to amino acid residues around phosphorylated Tyr460.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816854
Product Specific Information
Antibody detects endogenous levels of DR6 only when phosphorylated at Tyr460.
Target Information
DR6 is widely expressed in human tissues and cell lines. The ligand for DR6 has not been identified. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, and DR5 were recently identified. A new death domain containing receptor in the TNFR family was cloned recently and termed DR6 for death receptor-6. Like TNF-R1, DR6 interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kappa-B and JNK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD358; Death receptor 6; Death receptor-6; MGC31965; sCD358; sDR6; soluble CD358; soluble DR6; TNFR-related death receptor 6; Tumor necrosis factor receptor superfamily member 21; tumor necrosis factor receptor superfamily, member 21
Gene Aliases: BM-018; CD358; DR6; TNFRSF21; UNQ437/PRO868
UniProt ID: (Human) O75509, (Rat) D3ZF92
Entrez Gene ID: (Human) 27242, (Rat) 316256
B cell apoptotic process
adaptive immune response
apoptotic process
inflammatory response
immune response
humoral immune response
negative regulation of B cell proliferation
negative regulation of myelination
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
negative regulation of T cell proliferation
myelination
regulation of apoptotic process
regulation of oligodendrocyte differentiation
T cell receptor signaling pathway
neuron apoptotic process
cellular response to tumor necrosis factor
apoptotic signaling pathway
oligodendrocyte apoptotic process
negative regulation of interleukin-5 secretion
negative regulation of interleukin-13 secretion
negative regulation of interleukin-10 secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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