Invitrogen

Phospho-DRP1 (Ser616) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

13 References

View all (56) DNM1L antibodies

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Datasheet

Protocols

Questions & Answers

Phospho-DRP1 (Ser616) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

Phospho-DRP1 (Ser616) Antibody (PA5-64821) in ICC/IF

Immunofluorescence analysis of Phospho DRP1 was performed using 70% confluent log phase HeLa cells treated with nocodazole at 100 ng/mL for 17 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-DRP1 (Ser616) Polyclonal Antibody (Product # PA5-648... View More

Phospho-DRP1 (Ser616) Antibody in Western Blot (WB)

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Product Details

PA5-64821

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human DRP1 (Phospho-Ser616)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2663284

Product Specific Information

Phospho-DRP1 (Ser616) Polyclonal Antibody detects endogenous levels of DRP1 only when phosphorylated at Ser616.

Target Information

A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Grb2 Induces Cardiorenal Syndrome Type 3: Roles of IL-6, Cardiomyocyte Bioenergetics, and Akt/mTOR Pathway.

Frontiers in cell and developmental biology

Wang J,Sun X,Wang X,Cui S,Liu R,Liu J,Fu B,Gong M,Wang C,Shi Y,Chen Q,Cai G,Chen X

Published figure using Phospho-DRP1 (Ser616) polyclonal antibody (Product # PA5-64821) in Western Blot

Mon Sep 05 00:00:00 EDT 2022

Combined Cornus Officinalis and Paeonia Lactiflora Pall Therapy Alleviates Rheumatoid Arthritis by Regulating Synovial Apoptosis via AMPK-Mediated Mitochondrial Fission.

Frontiers in pharmacology

Huang L,Hu S,Shao M,Wu X,Zhang J,Cao G

PA5-64821 was used in Western Blot to compare the therapeutic effect of Cornus officinalis and Paeonia lactiflora with that of their main active compounds, ursolic acid and paeoniflorin, respectively, and investigate the potential involvement of AMPK-mediated mitochondrial dynamics in their therapeutic action.

Wed Apr 28 00:00:00 EDT 2021

Combined Cornus Officinalis and Paeonia Lactiflora Pall Therapy Alleviates Rheumatoid Arthritis by Regulating Synovial Apoptosis via AMPK-Mediated Mitochondrial Fission.

Frontiers in pharmacology

Huang L,Hu S,Shao M,Wu X,Zhang J,Cao G

Wed Apr 28 00:00:00 EDT 2021

Synapsin-Promoted Caveolin-1 Overexpression Maintains Mitochondrial Morphology and Function in PSAPP Alzheimer's Disease Mice.

Cells

Wang S,Ichinomiya T,Terada Y,Wang D,Patel HH,Head BP

PA5-64821 was used in Western Blotting to demonstrate the critical role for Cav-1 in maintaining normal mitochondrial morphology and function through affecting mitochondrial dynamics and explain a molecular and cellular mechanism underlying the previously reported neuroprotective and cognitive preservation induced by SynCav1 in PSAPP mouse model of AD.

Mon Sep 20 00:00:00 EDT 2021

Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes.

Clinical and translational medicine

He C,Gao P,Cui Y,Li Q,Li Y,Lu Z,Ma H,Zhao Y,Li L,Sun F,Chen X,Jia H,Liu D,Yang G,Zheng H,Zhu Z

PA5-64821 was used in Western Blotting to indicate that TRPC6 is a critical sensitive cation channel to hypoglycemia and is a promising target to prevent RH-induced cognitive impairment by properly orchestrating the mitochondrial dynamics in diabetic patients.

Thu Oct 01 00:00:00 EDT 2020

Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes.

Clinical and translational medicine

He C,Gao P,Cui Y,Li Q,Li Y,Lu Z,Ma H,Zhao Y,Li L,Sun F,Chen X,Jia H,Liu D,Yang G,Zheng H,Zhu Z

Thu Oct 01 00:00:00 EDT 2020

Mitochondrial Fission and Mitophagy Reciprocally Orchestrate Cardiac Fibroblasts Activation.

Frontiers in cell and developmental biology

Gao QY,Zhang HF,Tao J,Chen ZT,Liu CY,Liu WH,Wu MX,Yin WY,Gao GH,Xie Y,Yang Y,Liu PM,Wang JF,Chen YX

Published figure using Phospho-DRP1 (Ser616) polyclonal antibody (Product # PA5-64821) in Western Blot

Mon Sep 12 00:00:00 EDT 2022

Mitochondrial Fission and Mitophagy Reciprocally Orchestrate Cardiac Fibroblasts Activation.

Frontiers in cell and developmental biology

Gao QY,Zhang HF,Tao J,Chen ZT,Liu CY,Liu WH,Wu MX,Yin WY,Gao GH,Xie Y,Yang Y,Liu PM,Wang JF,Chen YX

Published figure using Phospho-DRP1 (Ser616) polyclonal antibody (Product # PA5-64821) in Western Blot

Mon Sep 12 00:00:00 EDT 2022

DRP1 Promotes BRAF^V600E-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer.

Frontiers in oncology

Padder RA,Bhat ZI,Ahmad Z,Singh N,Husain M

PA5-64821 was used in Western Blot to investigate how BRAFV600E driven CRC (colorectal cancer) progression is linked to mitochondrial fission/fusion dynamics and whether this window could be exploited to target CRC progression.

Thu Apr 21 00:00:00 EDT 2022

DRP1 Promotes BRAF^V600E-Driven Tumor Progression and Metabolic Reprogramming in Colorectal Cancer.

Frontiers in oncology

Padder RA,Bhat ZI,Ahmad Z,Singh N,Husain M

Thu Apr 21 00:00:00 EDT 2022

Haemin pre-treatment augments the cardiac protection of mesenchymal stem cells by inhibiting mitochondrial fission and improving survival.

Journal of cellular and molecular medicine

Deng R,Liu Y,He H,Zhang H,Zhao C,Cui Z,Hong Y,Li X,Lin F,Yuan D,Liang X,Zhang Y

PA5-64821 was used in Western Blotting to study regulation of mesenchymal stem cell survival by haemin.

Wed Jan 01 00:00:00 EST 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

PA5-64821 was used in Western Blot, Immunohistochemistry to find that inflicting acute lung injury (ALI) to mice doubles their incidence of AAA and accelerates macrophage-driven proteolytic damage of the aortic wall.

Thu Aug 27 00:00:00 EDT 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

Thu Aug 27 00:00:00 EDT 2020

Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages.

Nature communications

Boytard L,Hadi T,Silvestro M,Qu H,Kumpfbeck A,Sleiman R,Fils KH,Alebrahim D,Boccalatte F,Kugler M,Corsica A,Gelb BE,Jacobowitz G,Miller G,Bellini C,Oakes J,Silvestre JS,Zangi L,Ramkhelawon B

Thu Aug 27 00:00:00 EDT 2020

Adipose-Derived Mesenchymal Stem Cells Isolated from Patients with Abdominal Aortic Aneurysm Exhibit Senescence Phenomena.

Oxidative medicine and cellular longevity

Huang X,Zhang H,Liang X,Hong Y,Mao M,Han Q,He H,Tao W,Jiang G,Zhang Y,Li X

Published figure using Phospho-DRP1 (Ser616) polyclonal antibody (Product # PA5-64821) in Western Blot

Fri May 01 00:00:00 EDT 2020

FGF21 Mediates Mesenchymal Stem Cell Senescence via Regulation of Mitochondrial Dynamics.

Oxidative medicine and cellular longevity

Li X,Hong Y,He H,Jiang G,You W,Liang X,Fu Q,Han S,Lian Q,Zhang Y

PA5-64821 was used in Western Blotting to study the mechanisms of fibroblast growth factor 21 in the regulation of mesenchymal stem cell senescence.

Thu Jan 16 00:00:00 EST 2020

Vascular progenitor cell senescence in patients with Marfan syndrome.

Journal of cellular and molecular medicine

He H,Yu B,Liu Z,Ye G,You W,Hong Y,Lian Q,Zhang Y,Li X

PA5-64821 was used in Western Blotting to assess the function of Vascular progenitor cells (VPCs) isolated from patients with Marfan syndrome (MFS).

Sat Jun 01 00:00:00 EDT 2019

Cardiac Dysfunction in the Sigma 1 Receptor Knockout Mouse Associated With Impaired Mitochondrial Dynamics and Bioenergetics.

Journal of the American Heart Association

Abdullah CS,Alam S,Aishwarya R,Miriyala S,Panchatcharam M,Bhuiyan MAN,Peretik JM,Orr AW,James J,Osinska H,Robbins J,Lorenz JN,Bhuiyan MS

PA5-64821 was used in Western Blotting to investigate the physiological function of Sigmar1 in regulating cardiac hemodynamics using the Sigmar1 knockout mouse (Sigmar1-/-).

Tue Oct 16 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; N-terminal region; VPS1

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Gene Aliases: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; HDYNIV; python

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UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6

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Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006

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Function(s)

GTPase activity GTPase activator activity protein binding GTP binding lipid binding Rab GTPase binding GTP-dependent protein binding ubiquitin protein ligase binding protein homodimerization activity identical protein binding nucleotide binding hydrolase activity membrane traffic protein

Process(es)

mitochondrial fission release of cytochrome c from mitochondria dynamin polymerization involved in mitochondrial fission endocytosis cellular component disassembly involved in execution phase of apoptosis regulation of mitochondrion organization peroxisome fission regulation of protein oligomerization positive regulation of apoptotic process positive regulation of GTPase activity mitochondrial fragmentation involved in apoptotic process positive regulation of protein secretion protein oligomerization protein homotetramerization heart contraction membrane fusion necroptotic process mitochondrion morphogenesis protein localization to mitochondrion positive regulation of mitochondrial fission mitochondrial membrane fission positive regulation of release of cytochrome c from mitochondria regulation of peroxisome organization regulation of mitophagy regulation of ATP metabolic process positive regulation of intrinsic apoptotic signaling pathway metabolic process programmed cell death

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Phospho-DRP1 (Ser616) Polyclonal Antibody

Phospho-DRP1 (Ser616) Antibody (PA5-64821) in ICC/IF

Product Details

PA5-64821

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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