Invitrogen

Phospho-EGFR (Tyr1068) Monoclonal Antibody (S.684.2)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Product Details

MA5-15199

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100
-

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Human, Non-human primate, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

S.684.2

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pTyr1068 of human EGF receptor
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1865 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_10985111

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; EGFR-related peptide; Epidermal growth factor receptor; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone

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Gene Aliases: EGFR; ERBB; ErbB-1; ERBB1; Errp; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956, (Rat) 24329

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Function(s)
chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion glycoprotein binding signal transducer activity receptor binding integrin binding kinase activity protein kinase binding epidermal growth factor binding transmembrane signal receptor
Process(es)
MAPK cascade activation of MAPKK activity cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development response to stress signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway activation of phospholipase C activity salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion peptidyl-tyrosine phosphorylation cerebral cortex cell migration positive regulation of cell growth positive regulation of cell migration positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway negative regulation of protein catabolic process positive regulation of phosphorylation activation of phospholipase A2 activity by calcium-mediated signaling negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of nitric oxide biosynthetic process positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation regulation of nitric-oxide synthase activity protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of cell motility liver development translation response to osmotic stress response to oxidative stress circadian rhythm epidermis development magnesium ion homeostasis response to organic cyclic compound diterpenoid metabolic process signal transduction by protein phosphorylation lung development response to estradiol positive regulation of superoxide anion generation response to cobalamin response to hydroxyisoflavone response to lipid intracellular signal transduction cellular response to drug regulation of cell proliferation ovulation cycle hydrogen peroxide metabolic process tongue development positive regulation of vasoconstriction positive regulation of vasodilation negative regulation of mitotic cell cycle astrocyte activation positive regulation of smooth muscle cell proliferation neuron projection morphogenesis positive regulation of inflammatory response response to calcium ion positive regulation of synaptic transmission, glutamatergic cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to dexamethasone stimulus
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