Invitrogen

Phospho-EGFR (Tyr1173) Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

13 Published Figures

29 References

View all (279) EGFR antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Phospho-EGFR (Tyr1173) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

Phospho-EGFR (Tyr1173) Antibody (44-794G) in ICC/IF

Immunofluorescence analysis of EGFR (pY1173) was done on 70% confluent log phase HeLa cells treated with EGF (200 ng/mL for 10 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with EGFR (pY1173) Rabbit polyclonal Antibody (Product # 44-794G) at 2 µg/mL in 1... View More

Phospho-EGFR (Tyr1173) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-EGFR (Tyr1173) Antibody in peptide-ELISA (pep-ELISA)

Phospho-EGFR (Tyr1173) Antibody in Western Blot (WB)

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Product Details

44-794G

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:500

View 2 publications 2 publications

peptide-ELISA (pep-ELISA)

Assay-dependent

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bacteria, Human, Mouse, Sheep

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human EGFR that contains tyrosine 1173. The sequence is conserved in mouse and rat.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533757

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Flotillin-2 regulates epidermal growth factor receptor activation, degradation by Cbl-mediated ubiquitination, and cancer growth.

The Journal of biological chemistry

Wisniewski DJ,Liyasova MS,Korrapati S,Zhang X,Ratnayake S,Chen Q,Gilbert SF,Catalano A,Voeller D,Meerzaman D,Guha U,Porat-Shliom N,Annunziata CM,Lipkowitz S

Published figure using Phospho-EGFR (Tyr1173) polyclonal antibody (Product # 44-794G) in Western Blot

Sun Jan 01 00:00:00 EST 2023

Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.

Cancers

Moritz S,Krause M,Schlatter J,Cordes N,Vehlow A

44-794G was used in Western Blot to show that the cytoskeleton regulator Lamellipodin mediates invasiveness, proliferation and radiosensitivity of glioblastoma cells.

Sun Oct 24 00:00:00 EDT 2021

Lamellipodin-RICTOR Signaling Mediates Glioblastoma Cell Invasion and Radiosensitivity Downstream of EGFR.

Cancers

Moritz S,Krause M,Schlatter J,Cordes N,Vehlow A

44-794G was used in Western Blot to show that the cytoskeleton regulator Lamellipodin mediates invasiveness, proliferation and radiosensitivity of glioblastoma cells.

Sun Oct 24 00:00:00 EDT 2021

Cooperative Blockade of PKCα and JAK2 Drives Apoptosis in Glioblastoma.

Cancer research

Wong RA,Luo X,Lu M,An Z,Haas-Kogan DA,Phillips JJ,Shokat KM,Weiss WA,Fan QW

44-794G was used in Western Blotting to investigate targets for cancer treatments found within mTOR inhibitors and their drivers.

Sat Feb 15 00:00:00 EST 2020

Adenoviral transduction of EGFR into pregnancy-adapted uterine artery endothelial cells remaps growth factor induction of endothelial dysfunction.

Molecular and cellular endocrinology

Clemente L,Boeldt DS,Grummer MA,Morita M,Morgan TK,Wiepz GJ,Bertics PJ,Bird IM

44-794G was used in Western Blotting to suggest that placenta derived exosomes positive for EGFR should be further considered as a possible cause of endothelial dysfunction in women with PE.

Wed Jan 01 00:00:00 EST 2020

M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB‑EGF.

Oncology reports

Fu E,Liu T,Yu S,Chen X,Song L,Lou H,Ma F,Zhang S,Hussain S,Guo J,Sun J,Yu P,Mao X,Wei L

44-794G was used in Western Blotting to examine the potential role of human heparin-binding epidermal growth factor (HB-EGF) secreted by M2 macrophages in the development of radioresistance in head and neck squamous cell carcinoma (HNSCC).

Sat Aug 01 00:00:00 EDT 2020

Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets.

PloS one

Korolkova OY,Widatalla SE,Whalen DS,Nangami GN,Abimbola A,Williams SD,Beasley HK,Reisenbichler E,Washington MK,Ochieng J,Mayer IA,Lehmann BD,Sakwe AM

44-794G was used in Immunohistochemistry-immunofluorescence to establish that the reciprocal expression of annexin A6 (AnxA6) and RasGRF2 is dependent on inhibition of non-selective Ca2+ channels in AnxA6-low but not AnxA6-high TNBC cells.

Mon Aug 03 00:00:00 EDT 2020

Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets.

PloS one

Korolkova OY,Widatalla SE,Whalen DS,Nangami GN,Abimbola A,Williams SD,Beasley HK,Reisenbichler E,Washington MK,Ochieng J,Mayer IA,Lehmann BD,Sakwe AM

Mon Aug 03 00:00:00 EDT 2020

Evidence for functional selectivity in TUDC- and norUDCA-induced signal transduction via α₅β₁ integrin towards choleresis.

Scientific reports

Bonus M,Sommerfeld A,Qvartskhava N,Görg B,Ludwig BS,Kessler H,Gohlke H,Häussinger D

Published figure using Phospho-EGFR (Tyr1173) polyclonal antibody (Product # 44-794G) in Western Blot

Thu Apr 02 00:00:00 EDT 2020

Cell-penetrable nanobodies (transbodies) that inhibit the tyrosine kinase activity of EGFR leading to the impediment of human lung adenocarcinoma cell motility and survival.

Journal of cellular biochemistry

Tabtimmai L,Suphakun P,Srisook P,Kiriwan D,Phanthong S,Kiatwuthinon P,Chaicumpa W,Choowongkomon K

44-794G was used in Immunocytochemistry, Immunohistochemistry (Paraffin), peptide-ELISA, Western Blot to study humanized-cell penetrable nanobodies.

Tue Oct 01 00:00:00 EDT 2019

Appropriate Sequence for Afatinib and Cisplatin Combination Improves Anticancer Activity in Head and Neck Squamous Cell Carcinoma.

Frontiers in oncology

Longton E,Schmit K,Fransolet M,Clement F,Michiels C

44-794G was used in Western Blotting to investigate the anti-tumour activity of afatinib combined to cisplatin in different schedules of exposure in Head and Neck Squamous Cell Carcinoma.

Tue Sep 29 00:00:00 EDT 2020

Fc-mediated activity of EGFR x c-Met bispecific antibody JNJ-61186372 enhanced killing of lung cancer cells.

mAbs

Grugan KD,Dorn K,Jarantow SW,Bushey BS,Pardinas JR,Laquerre S,Moores SL,Chiu ML

44794G was used in western blot to elucidate how JNJ-61186372 attenuates therapeutic resistance in patients with epidermal growth factor receptor mutations

Sun Jan 01 00:00:00 EST 2017

The Progestin Receptor Interactome in the Female Mouse Hypothalamus: Interactions with Synaptic Proteins Are Isoform Specific and Ligand Dependent.

eNeuro

Acharya KD,Nettles SA,Sellers KJ,Im DD,Harling M,Pattanayak C,Vardar-Ulu D,Lichti CF,Huang S,Edwards DP,Srivastava DP,Denner L,Tetel MJ

44-794G was used in Western Blot to provide evidence for novel functions of progestin receptor (PR) in synaptic modulation and energy homeostasis to affect health and disease.

Wed May 30 00:00:00 EDT 2018

A Monoclonal Antibody to ADAM17 Inhibits Tumor Growth by Inhibiting EGFR and Non-EGFR-Mediated Pathways.

Molecular cancer therapeutics

Rios-Doria J,Sabol D,Chesebrough J,Stewart D,Xu L,Tammali R,Cheng L,Du Q,Schifferli K,Rothstein R,Leow CC,Heidbrink-Thompson J,Jin X,Gao C,Friedman J,Wilkinson B,Damschroder M,Pierce AJ,Hollingsworth RE,Tice DA,Michelotti EF

44-794G was used in western blot to study the effect of using an ADAM17 inhibitory antibody, MEDI3622, in several EGFR-dependent tumor models.

Wed Jul 01 00:00:00 EDT 2015

Opposing Effects of Inhibitors of Aurora-A and EGFR in Autosomal-Dominant Polycystic Kidney Disease.

Frontiers in oncology

Nikonova AS,Deneka AY,Eckman L,Kopp MC,Hensley HH,Egleston BL,Golemis EA

44-794G was used in western blot to investigate changes to AURKA signaling after treatment with erlotinib/alisertib combination therapy

Tue Nov 03 00:00:00 EST 2015

Opposing Effects of Inhibitors of Aurora-A and EGFR in Autosomal-Dominant Polycystic Kidney Disease.

Frontiers in oncology

Nikonova AS,Deneka AY,Eckman L,Kopp MC,Hensley HH,Egleston BL,Golemis EA

44-794G was used in western blot to investigate changes to AURKA signaling after treatment with erlotinib/alisertib combination therapy

Tue Nov 03 00:00:00 EST 2015

Compounds identified by virtual docking to a tetrameric EGFR extracellular domain can modulate Grb2 internalization.

BMC cancer

Ramirez UD,Nikonova AS,Liu H,Pecherskaya A,Lawrence SH,Serebriiskii IG,Zhou Y,Robinson MK,Einarson MB,Golemis EA,Jaffe EK

44-794G was used in western blot to discuss novel EGFR-targeting therapeutics to treat cancer.

Thu May 28 00:00:00 EDT 2015

Intracellular activation of EGFR by fatty acid synthase dependent palmitoylation.

Oncotarget

Bollu LR,Katreddy RR,Blessing AM,Pham N,Zheng B,Wu X,Weihua Z

44-794G was used in western blot to elucidate the mechanism of intracellular activation of EGFR by fatty acid synthase dependent palmitoylation

Tue Oct 27 00:00:00 EDT 2015

Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells.

Cell cycle (Georgetown, Tex.)

Bollu LR,Ren J,Blessing AM,Katreddy RR,Gao G,Xu L,Wang J,Su F,Weihua Z

44-794G was used in immunocytochemistry and western blot to analyze EGF induced mitochondrial fusion of cancer cells created by de novo synthesized palmitate and mitochondrial EGFR

Thu Aug 13 00:00:00 EDT 2015

Inhibiting heat shock protein 90 (HSP90) limits the formation of liver cysts induced by conditional deletion of Pkd1 in mice.

PloS one

Smithline ZB,Nikonova AS,Hensley HH,Cai KQ,Egleston BL,Proia DA,Seeger-Nukpezah T,Golemis EA

44-794G was used in western blot to determine the effect of STA-2842 on liver size and cystic burden in Pkd-/- mice with established polycystic liver disease.

Tue Dec 29 00:00:00 EST 2015

Identification of prognostic biomarkers for glioblastomas using protein expression profiling.

International journal of oncology

Jung Y,Joo KM,Seong DH,Choi YL,Kong DS,Kim Y,Kim MH,Jin J,Suh YL,Seol HJ,Shin CS,Lee JI,Kim JH,Song SY,Nam DH

44-794G was used in immunohistochemistry - paraffin section to identify diagnostic and prognostic markers for glioblastoma

Sun Apr 01 00:00:00 EDT 2012

Characterization of kinase inhibitors using reverse phase protein arrays.

Methods in molecular biology (Clifton, N.J.)

Martiny-Baron G,Haasen D,D'Dorazio D,Voshol J,Fabbro D

44-794G was used in western blot to use reverse protein arrays to profile kinase inhibitors in various cellular pathways.

Tue Jan 03 00:00:00 EST 2012

Enhanced activation of epidermal growth factor receptor caused by tumor-derived E-cadherin mutations.

Cancer research

Bremm A,Walch A,Fuchs M,Mages J,Duyster J,Keller G,Hermannstädter C,Becker KF,Rauser S,Langer R,von Weyhern CH,Höfler H,Luber B

Fri Feb 01 00:00:00 EST 2008

Enhanced activation of epidermal growth factor receptor caused by tumor-derived E-cadherin mutations.

Cancer research

Bremm A,Walch A,Fuchs M,Mages J,Duyster J,Keller G,Hermannstädter C,Becker KF,Rauser S,Langer R,von Weyhern CH,Höfler H,Luber B

Fri Feb 01 00:00:00 EST 2008

Enhanced activation of epidermal growth factor receptor caused by tumor-derived E-cadherin mutations.

Cancer research

Bremm A,Walch A,Fuchs M,Mages J,Duyster J,Keller G,Hermannstädter C,Becker KF,Rauser S,Langer R,von Weyhern CH,Höfler H,Luber B

Fri Feb 01 00:00:00 EST 2008

Enhanced activation of epidermal growth factor receptor caused by tumor-derived E-cadherin mutations.

Cancer research

Bremm A,Walch A,Fuchs M,Mages J,Duyster J,Keller G,Hermannstädter C,Becker KF,Rauser S,Langer R,von Weyhern CH,Höfler H,Luber B

Fri Feb 01 00:00:00 EST 2008

Tumor growth inhibition with cetuximab and chemotherapy in non-small cell lung cancer xenografts expressing wild-type and mutated epidermal growth factor receptor.